GLE1L

GLE1 RNA export mediator
Identifiers
Symbols	GLE1 ; GLE1L; LCCS; LCCS1; hGLE1
External IDs	OMIM: 603371 MGI: 1921662 HomoloGene: 20379 GeneCards: GLE1 Gene
Gene ontology Molecular function • protein binding • identical protein binding Cellular component • extracellular space • nuclear pore • cytoplasm • plasma membrane • membrane Biological process • mRNA export from nucleus • protein transport • poly(A)+ mRNA export from nucleus Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	2733	74412
Ensembl	ENSG00000119392	ENSMUSG00000019715
UniProt	Q53GS7	Q8R322
RefSeq (mRNA)	NM_001003722	NM_028923
RefSeq (protein)	NP_001003722	NP_083199
Location (UCSC)	Chr 9: 128.5 – 128.54 Mb	Chr 2: 29.94 – 29.96 Mb
PubMed search

Nucleoporin GLE1 is a protein that in humans is encoded by the GLE1 gene on chromosome 9.^[1][2][3]

Function

This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.^[3]

Clinical significance

A genome-wide screening and linkage analysis assigned the disease locus of lethal congenital contracture syndrome, one of 40 Finnish heritage diseases, to a defined region of 9q34, where the GLE1 gene is located.^[4] Mutations in GLEI have been identified in families with foetal motoneuron disease.^[5]

Interactions

GLE1L has been shown to interact with NUP155.^[6]

References

1. Watkins JL, Murphy R, Emtage JL, Wente SR (Jul 1998). "The human homologue of Saccharomyces cerevisiae Gle1p is required for poly(A)+ RNA export". Proc Natl Acad Sci U S A 95 (12): 6779–84. doi:10.1073/pnas.95.12.6779. PMC 22633. PMID 9618489.
2. Nousiainen HO, Kestilä M, Pakkasjärvi N, Honkala H, Kuure S, Tallila J, Vuopala K, Ignatius J, Herva R, Peltonen L (Jan 2008). "Mutations in mRNA export mediator GLE1 result in a fetal motoneuron disease". Nat Genet 40 (2): 155–7. doi:10.1038/ng.2007.65. PMC 2684619. PMID 18204449.
3. "Entrez Gene: GLE1L GLE1 RNA export mediator-like (yeast)".
4. Mäkelä-Bengs P, Järvinen N, Vuopala K, Suomalainen A, Palotie A, Peltonen L (1997). "The assignment the lethal congenital contracture syndrome (LCCS) locus to chromosome 9q33-34". Am. J. Hum. Genet. 61 (suppl): A30.
5. Nousiainen HO, Kestilä M, Pakkasjärvi N, Honkala H, Kuure S, Tallila J, Vuopala K, Ignatius J, Herva R, Peltonen L (February 2008). "Mutations in mRNA export mediator GLE1 result in a fetal motoneuron disease". Nature Genetics 40 (2): 155–157. doi:10.1038/ng.2007.65. PMC 2684619. PMID 18204449.
6. Rayala HJ, Kendirgi F, Barry DM, Majerus PW, Wente SR (Feb 2004). "The mRNA export factor human Gle1 interacts with the nuclear pore complex protein Nup155". Mol. Cell Proteomics 3 (2): 145–55. doi:10.1074/mcp.M300106-MCP200. PMID 14645504.

Further reading

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Kendirgi F, Barry DM, Griffis ER, Powers MA, Wente SR (2003). "An essential role for hGle1 nucleocytoplasmic shuttling in mRNA export". J. Cell Biol. 160 (7): 1029–40. doi:10.1083/jcb.200211081. PMC 2172758. PMID 12668658.
• Rayala HJ, Kendirgi F, Barry DM, Majerus PW, Wente SR (2004). "The mRNA export factor human Gle1 interacts with the nuclear pore complex protein Nup155". Mol. Cell Proteomics 3 (2): 145–55. doi:10.1074/mcp.M300106-MCP200. PMID 14645504.
• Kendirgi F, Rexer DJ, Alcázar-Román AR, Onishko HM, Wente SR (2006). "Interaction between the Shuttling mRNA Export Factor Gle1 and the Nucleoporin hCG1: A Conserved Mechanism in the Export of Hsp70 mRNA". Mol. Biol. Cell 16 (9): 4304–15. doi:10.1091/mbc.E04-11-0998. PMC 1196339. PMID 16000379.