GTF3C5

General transcription factor IIIC, polypeptide 5, 63kDa
Identifiers
Symbols	GTF3C5 ; TFIIIC63; TFIIICepsilon; TFiiiC2-63
External IDs	OMIM: 604890 MGI: 1917489 HomoloGene: 40806 GeneCards: GTF3C5 Gene
Gene ontology Molecular function • DNA binding • protein binding Cellular component • transcription factor TFIIIC complex • nucleoplasm Biological process • transcription, DNA-templated • transcription from RNA polymerase III promoter • gene expression • skeletal muscle cell differentiation • 5S class rRNA transcription from RNA polymerase III type 1 promoter • tRNA transcription from RNA polymerase III promoter Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	9328	70239
Ensembl	ENSG00000148308	ENSMUSG00000026816
UniProt	Q9Y5Q8	Q8R2T8
RefSeq (mRNA)	NM_001122823	NM_001290484
RefSeq (protein)	NP_001116295	NP_001277413
Location (UCSC)	Chr 9: 133.03 – 133.06 Mb	Chr 2: 28.57 – 28.58 Mb
PubMed search

General transcription factor 3C polypeptide 5 is a protein that in humans is encoded by the GTF3C5 gene.^[1][2]

Model organisms

Gtf3c5 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Abnormal
Recessive lethal study	Abnormal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology	Normal
Peripheral blood lymphocytes	Normal
Micronucleus test	Normal
Heart weight	Normal
Eye Histopathology	Normal
Salmonella infection	Normal[3]
Citrobacter infection	Normal[4]
All tests and analysis from[5][6]

Model organisms have been used in the study of GTF3C5 function. A conditional knockout mouse line, called Gtf3c5^{tm2a(KOMP)Wtsi[7][8]} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.^[9][10][11]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.^[5][12] Twenty four tests were carried out on mutant mice and two significant abnormalities were observed.^[5] No homozygous mutant embryos were identified during gestation, and therefore none survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice; no additional significant abnormalities were observed in these animals.^[5]

Interactions

GTF3C5 has been shown to interact with GTF3C2^[1] and GTF3C4.^[13]

References

1. Hsieh YJ, Wang Z, Kovelman R, Roeder RG (Jul 1999). "Cloning and characterization of two evolutionarily conserved subunits (TFIIIC102 and TFIIIC63) of human TFIIIC and their involvement in functional interactions with TFIIIB and RNA polymerase III". Mol. Cell. Biol. 19 (7): 4944–52. PMC 84305. PMID 10373544.
2. "Entrez Gene: GTF3C5 general transcription factor IIIC, polypeptide 5, 63kDa".
3. "Salmonella infection data for Gtf3c5". Wellcome Trust Sanger Institute.
4. "Citrobacter infection data for Gtf3c5". Wellcome Trust Sanger Institute.
5. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
6. Mouse Resources Portal, Wellcome Trust Sanger Institute.
7. "International Knockout Mouse Consortium".
8. "Mouse Genome Informatics".
9. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
10. Dolgin E (2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
11. Collins FS, Rossant J, Wurst W (2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
12. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biol. 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
13. Hsieh YJ, Kundu TK, Wang Z, Kovelman R, Roeder RG (Nov 1999). "The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone-specific acetyltransferase activity". Mol. Cell. Biol. 19 (11): 7697–704. PMC 84812. PMID 10523658.

Further reading

• Jang KL, Collins MK, Latchman DS (1992). "The human immunodeficiency virus tat protein increases the transcription of human Alu repeated sequences by increasing the activity of the cellular transcription factor TFIIIC". J. Acquir. Immune Defic. Syndr. 5 (11): 1142–7. PMID 1403646.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474.
• Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA (1997). "Large-scale concatenation cDNA sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Hsieh YJ, Kundu TK, Wang Z, Kovelman R, Roeder RG (1999). "The TFIIIC90 subunit of TFIIIC interacts with multiple components of the RNA polymerase III machinery and contains a histone-specific acetyltransferase activity". Mol. Cell. Biol. 19 (11): 7697–704. PMC 84812. PMID 10523658.
• Dumay-Odelot H, Marck C, Durrieu-Gaillard S, Lefebvre O, Jourdain S, Prochazkova M, Pflieger A, Teichmann M (2007). "Identification, molecular cloning, and characterization of the sixth subunit of human transcription factor TFIIIC". J. Biol. Chem. 282 (23): 17179–89. doi:10.1074/jbc.M611542200. PMID 17409385.