Gag/pol translational readthrough site

Gag/pol translational readthrough site (or Retroviral readthrough element) is a cis-regulatory element found in retroviruses.^[1] The readthrough site facilitates the mechanism of translation readthrough of the stop codon at the gag-pol junction producing the gag and pol fusion protein in certain retroviruses. Retroviruses whose gag and pol genes are in the same reading frame often depend upon approximately 5% read-through of the gag UAG termination codon to form the gag-pol polyprotein. This readthrough is usually dependent on a pseudoknot located eight nucleotides downstream of the stop codon (UAG). Sequence conservation is found in the second pseudoknot loop.

Gallery of secondary structure images

GP_knot1: Secondary structure taken from the Rfam database. Family RF01073. Derived from Pseudobase PKB00047 PKB00078 PKB00079 PKB00088 PKB00098 PKB00099

GP_knot2: Secondary structure taken from the Rfam database. Family RF01092. Derived from Pseudobase PKB00048

References

↑ Wills NM, Gesteland RF, Atkins JF (September 1994). "Pseudoknot-dependent read-through of retroviral gag termination codons: importance of sequences in the spacer and loop 2". EMBO J. 13 (17): 4137–44. PMC 395336. PMID 8076609.

External links

Page for GP_knot1 at Rfam

Page for GP_knot2 at Rfam
Pseudobase

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