HHV Latency Associated Transcript

HHV Latency Associated Transcript (HHV LAT) is a length of RNA which accumulates in cells hosting long-term, or latent, Human Herpes Virus (HHV) infections. The LAT RNA is produced by genetic transcription from a certain region of the viral DNA. LAT regulates the viral genome and interferes with the normal activities of the infected host cell.

Herpes virus may establish lifelong infection during which a reservoir virus population survives in host nerve cells for long periods of time. Such long-term Herpes infection requires a mode of cellular infection known as latent infection. During the latent infection, the metabolism of the host cell is disrupted. While the infected cell would ordinarily undergo an organized death or be removed by the immune system, the consequences of LAT production interfere with these normal processes.

Latency is distinguished from lytic infection; in lytic infection many Herpes virus particles are produced and then burst or lyse the host cell. Lytic infection is sometimes known as "productive" infection. Latent cells harbor the virus for long time periods, then occasionally convert to productive infection which may lead to a recurrence of symptomatic Herpes symptoms.

During latency, most of the Herpes DNA is inactive, with the exception of LAT, which accumulates within infected cells. The region of HHV DNA which encodes LAT is known as LAT-DNA. After splicing, LAT is a 2.0-kilobase transcript (or intron) produced from the 8.3-kb LAT-DNA. The DNA region containing LAT-DNA is known as the Latency Associated Transcript Region.[1]

Apoptosis is the process of normal cell death. In order to maintain a reservoir of latently infected host cells, Herpes virus interferes with apoptosis. HSV-1 LAT expression was once thought to produce interfering micro-RNA (miRNA) which suppress production of Human apoptosis-pathway proteins such as TGF-β1 and SMAD3,[2] but these findings were retracted by the researchers in January 2008.[3] HHV-8 LAT expression similarly produces miRNAs which suppress production of Thrombospondin-1 protein involved in apoptosis and angiogenesis.[4] Expression of LAT also reduces the production of other proteins involved in the apoptosis mechanism, including proteins caspase-8 and caspase-9.[5]

LAT expression regulates the activity of the Herpes genome during latent infection. LAT expression results in the suppression of Herpes lytic genes.[6] Genetic studies of the LAT-DNA have identified a portion known as a chromatin insulator which forms a boundary between activated LAT-DNA and the inactive lytic viral DNA.[7]

LAT regulates the expression of lytic genes

HHV Infected Cell Polypeptide 0 (ICP0) gene is expressed very early during lytic infection, and for this reason is called an immediate-early Herpes gene. In 1991, Farrell and colleagues report that the 2.0-kb LAT intron terminates at the 5' end with a 750-base antisense RNA complement for the ICP0 gene.[1]

In 2005, Qing-Yin Wang and colleagues from Harvard Medical School concluded, using assays comparing LAT-negative vs. LAT-positive virus strains, that expression of LAT in neurons represses the expression of several lytic gene products, including ICP4 and Thymidine Kinase. LAT expression results in changes to Histones, thus converting portions of viral DNA into a non-productive form known as heterochromatin.[6]

Simian varicella virus (SVV) is a Varicellovirus (a Genus of Subfamily Alphaherpesvirinae) which expresses an HHV LAT homolog known as SVV LAT, and an HHV ICP0 analog known as SVV-ORF61 (Open Reading Frame). SVV LAT is encoded such that it contains an antisense copy of SVV-ORF61 and that expression of SVV LAT during latency downregulates expression of ORF61 and other immediate-early SVV gene products.[8]

LAT DNA contains an activation boundary called a chromatin insulator

CCCTC-binding factor (CTCF) is a zinc finger protein which occurs naturally in some human cells. CTCF is localized to the nucleus of cells. CTCF has been shown [9] to naturally regulate the expression of human linear dsDNA by binding with target DNA sequences or motifs. CTCF binding to DNA may result in formation of transcription-ready euchromatin through the Histone H3-acetylating activity which results due to CTCF binding. Acetylation of Histone promotes transcription of DNA to RNA, and then to protein products.[9]

A March 2006 University of Florida College of Medicine study showed that expression of the Herpes virus genome may be regulated in part by the binding of CTCF to CTCF-binding motifs. The researchers used sequence analysis and quantitative genomics assays on HHV DNA. In the U. Florida study, the LAT region was found to contain a CTCF-binding region within a 1.5k-bp (base pair) region, and found to contain a "chromatin insulator-like element".[7] A May 2007 study conducted at the Wistar Institute localized the LAT CTCF-binding motif to an 800-bp sequence of the LAT intron, and demonstrated that the region insulated activated LAT chromatin from repressed chromatin that would otherwise produce the lytic protein HHV Infected Cell Polypeptide 0 (ICP0).[10]

LAT miRNA interferes with host cell apoptosis

The paper on which this section is based has been retracted recently. Apparently mir-LAT is not a viral miRNA. See http://www.nature.com/nature/journal/v451/n7178/full/nature06621.html for more details

Research has shown that a portion of HSV-1 LAT consists of an interfering micro RNA (miRNA). This miRNA was termed miR-LAT, and shown to downregulate Transforming Growth Factor-β1 (TGF-β1) and SMAD3. These effects block apoptosis, or normal programmed cell death.[2] Further research has shown that HHV-8 LAT produces miRNA which interfere not with expression of TGF-β1 and SMAD3, but reducing the expression of Thrombospondin-1 protein (THBS-1). In turn, down-regulation of THBS-1 reduces production of TGF-β1 and SMAD3, suppressing apoptosis.[4]

Other research showed that the products from the first 4,658 nucleotides of LAT inhibited caspase- 8 and caspase-9 cellular death factors.[5]

Footnotes

  1. 1 2 Farrell MJ, Dobson AT, Feldman LT (1991-02-01). "Herpes simplex virus latency-associated transcript is a stable intron". Proceedings of the National Academy of Sciences 88 (3): 790–4. doi:10.1073/pnas.88.3.790. PMC 50899. PMID 1846963. (Free full-text article: )
  2. 1 2 Gupta A, Gartner JJ, Sethupathy P, Hatzigeorgiou AG, Fraser NW (31 May 2006). "Anti-apoptotic function of a microRNA encoded by the HSV-1 latency-associated transcript". Nature 442 (7098): 82–5. doi:10.1038/nature04836. PMID 16738545. (Retracted. If this is intentional, please replace {{Retracted}} with {{Retracted|intentional=yes}}.)(Full-text: )
  3. ↑ Gartner JJ, Sethupathy P, Hatzigeorgiou AG, Fraser NW (Jan 31 2008). Retraction in Nature 451 (7178):600.
  4. 1 2 Samols MA, Skalsky RL, Maldonado AM, Riva A, Lopez MC, et al. "Identification of Cellular Genes Targeted by KSHV-Encoded MicroRNAs". PLoS Pathogens 3 (5). doi:10.1371/journal.ppat.0030065.
  5. 1 2 Henderson G, Peng W, Jin L, Perng GC, Nesburn AB, Wechsler SL, Jones C (December 2002). "Regulation of caspase 8- and caspase 9-induced apoptosis by the herpes simplex virus type 1 latency-associated transcript". Journal of Neurovirology 8 (2): 103–11. doi:10.1080/13550280290101085. PMID 12491160.
  6. 1 2 Wang QY, Zhou C, Johnson KE, Colgrove RC, Coen DM, Knipe DM (1 Nov 2005). "Herpesviral latency-associated transcript gene promotes assembly of heterochromatin on viral lytic-gene promoters in latent infection". Proceedings of the National Academy of Sciences 102 (44): 16055–9. doi:10.1073/pnas.0505850102. PMC 1266038. PMID 16247011. (Free full-text: )
  7. 1 2 Amelio AL, McAnany PK, Bloom DC (March 2006). "A chromatin insulator-like element in the herpes simplex virus type 1 latency-associated transcript region binds CCCTC-binding factor and displays enhancer-blocking and silencing activities". Journal of Virology 80 (5): 2358–68. doi:10.1128/JVI.80.5.2358-2368.2006. PMC 1395413. PMID 16474142.
  8. ↑ Ou Y, Davis KA, Traina-Dorge V, Gray WL (2007-05-16). "Simian varicella virus expresses a latency associated transcript that is antisense to ORF 61 (ICP0) mRNA in neural ganglia of latently infected monkeys". Journal of Virology 81 (15): 8149–56. doi:10.1128/JVI.00407-07. PMC 1951321. PMID 17507490.
  9. 1 2 "Homolog Report: CTCF". Rat Genome Database.
  10. ↑ Chen Q, Lin L, Smith S, Huang J, Berger SL, Zhou J (May 2007). "CTCF-Dependent Chromatin Boundary Element between the Latency-Associated Transcript and ICP0 Promoters in the Herpes Simplex Virus Type 1 Genome". Journal of Virology 81 (10): 5192–201. doi:10.1128/JVI.02447-06. PMC 1900208. PMID 17267480.
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