HIF1AN

Hypoxia inducible factor 1, alpha subunit inhibitor
PDB rendering based on 1h2k.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1H2K​, 1H2L​, 1H2M​, 1H2N​, 1IZ3​, 1MZE​, 1MZF​, 1YCI​, 2CGN​, 2CGO​, 2ILM​, 2W0X​, 2WA3​, 2WA4​, 2XUM​, 2Y0I​, 2YC0​, 2YDE​, 3D8C​, 3KCX​, 3KCY​, 3OD4​, 3P3N​, 3P3P​, 4AI8​, 4B7E​, 4B7K​, 4BIO​, 4JAA​, 4NR1​
Identifiers
Symbols	HIF1AN ; FIH1
External IDs	OMIM: 606615 MGI: 2442345 HomoloGene: 9906 ChEMBL: 5909 GeneCards: HIF1AN Gene
EC number	1.14.11.30, 1.14.11.n4
Gene ontology Molecular function • Notch binding • iron ion binding • protein binding • zinc ion binding • oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors • oxygen sensor activity • carboxylic acid binding • peptidyl-histidine dioxygenase activity • peptidyl-asparagine 3-dioxygenase activity • protein homodimerization activity • cofactor binding • NF-kappaB binding • ankyrin repeat binding Cellular component • nucleus • nucleoplasm • cytoplasm • cytosol • perinuclear region of cytoplasm Biological process • transcription, DNA-templated • peptidyl-histidine hydroxylation • peptidyl-aspartic acid hydroxylation • peptidyl-asparagine hydroxylation • positive regulation of myoblast differentiation • negative regulation of Notch signaling pathway • oxidation-reduction process • regulation of transcription from RNA polymerase II promoter in response to hypoxia • negative regulation of transcription from RNA polymerase II promoter in response to hypoxia • cellular response to hypoxia • positive regulation of vasculogenesis Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	55662	319594
Ensembl	ENSG00000166135	ENSMUSG00000036450
UniProt	Q9NWT6	Q8BLR9
RefSeq (mRNA)	NM_017902	NM_176958
RefSeq (protein)	NP_060372	NP_795932
Location (UCSC)	Chr 10: 100.53 – 100.56 Mb	Chr 19: 44.56 – 44.58 Mb
PubMed search

Hypoxia-inducible factor 1-alpha inhibitor is a protein that in humans is encoded by the HIF1AN gene.^[1][2]

Interactions

HIF1AN has been shown to interact with HIF1A^[1] and Von Hippel-Lindau tumor suppressor.^[1]

References

1. Mahon PC, Hirota K, Semenza GL (Oct 2001). "FIH-1: a novel protein that interacts with HIF-1α and VHL to mediate repression of HIF-1 transcriptional activity". Genes Dev 15 (20): 2675–86. doi:10.1101/gad.924501. PMC 312814. PMID 11641274.
2. "Entrez Gene: HIF1AN hypoxia-inducible factor 1, alpha subunit inhibitor".

Further reading

• Lando D, Peet DJ, Whelan DA, Gorman JJ, Whitelaw ML (2002). "Asparagine hydroxylation of the HIF transactivation domain a hypoxic switch". Science 295 (5556): 858–61. doi:10.1126/science.1068592. PMID 11823643.
• Freedman SJ, Sun ZY, Poy F, Kung AL, Livingston DM, Wagner G, Eck MJ (2002). "Structural basis for recruitment of CBP/p300 by hypoxia-inducible factor-1α". Proc. Natl. Acad. Sci. U.S.A. 99 (8): 5367–72. doi:10.1073/pnas.082117899. PMC 122775. PMID 11959990.
• Hewitson KS, McNeill LA, Riordan MV, Tian YM, Bullock AN, Welford RW, Elkins JM, Oldham NJ, Bhattacharya S, Gleadle JM, Ratcliffe PJ, Pugh CW, Schofield CJ (2002). "Hypoxia-inducible factor (HIF) asparagine hydroxylase is identical to factor inhibiting HIF (FIH) and is related to the cupin structural family". J. Biol. Chem. 277 (29): 26351–5. doi:10.1074/jbc.C200273200. PMID 12042299.
• Lando D, Peet DJ, Gorman JJ, Whelan DA, Whitelaw ML, Bruick RK (2002). "FIH-1 is an asparaginyl hydroxylase enzyme that regulates the transcriptional activity of hypoxia-inducible factor". Genes Dev. 16 (12): 1466–71. doi:10.1101/gad.991402. PMC 186346. PMID 12080085.
• Dann CE, Bruick RK, Deisenhofer J (2003). "Structure of factor-inhibiting hypoxia-inducible factor 1: An asparaginyl hydroxylase involved in the hypoxic response pathway". Proc. Natl. Acad. Sci. U.S.A. 99 (24): 15351–6. doi:10.1073/pnas.202614999. PMC 137720. PMID 12432100.
• Elkins JM, Hewitson KS, McNeill LA, Seibel JF, Schlemminger I, Pugh CW, Ratcliffe PJ, Schofield CJ (2003). "Structure of factor-inhibiting hypoxia-inducible factor (HIF) reveals mechanism of oxidative modification of HIF-1 alpha". J. Biol. Chem. 278 (3): 1802–6. doi:10.1074/jbc.C200644200. PMID 12446723.
• Lee C, Kim SJ, Jeong DG, Lee SM, Ryu SE (2003). "Structure of human FIH-1 reveals a unique active site pocket and interaction sites for HIF-1 and von Hippel-Lindau". J. Biol. Chem. 278 (9): 7558–63. doi:10.1074/jbc.M210385200. PMID 12482756.
• Koivunen P, Hirsilä M, Günzler V, Kivirikko KI, Myllyharju J (2004). "Catalytic properties of the asparaginyl hydroxylase (FIH) in the oxygen sensing pathway are distinct from those of its prolyl 4-hydroxylases". J. Biol. Chem. 279 (11): 9899–904. doi:10.1074/jbc.M312254200. PMID 14701857.
• Linke S, Stojkoski C, Kewley RJ, Booker GW, Whitelaw ML, Peet DJ (2004). "Substrate requirements of the oxygen-sensing asparaginyl hydroxylase factor-inhibiting hypoxia-inducible factor". J. Biol. Chem. 279 (14): 14391–7. doi:10.1074/jbc.M313614200. PMID 14734545.
• Kato H, Tamamizu-Kato S, Shibasaki F (2004). "Histone deacetylase 7 associates with hypoxia-inducible factor 1alpha and increases transcriptional activity". J. Biol. Chem. 279 (40): 41966–74. doi:10.1074/jbc.M406320200. PMID 15280364.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Cockman ME, Lancaster DE, Stolze IP, Hewitson KS, McDonough MA, Coleman ML, Coles CH, Yu X, Hay RT, Ley SC, Pugh CW, Oldham NJ, Masson N, Schofield CJ, Ratcliffe PJ (2006). "Posttranslational hydroxylation of ankyrin repeats in IκB proteins by the hypoxia-inducible factor (HIF) asparaginyl hydroxylase, factor inhibiting HIF (FIH)". Proc. Natl. Acad. Sci. U.S.A. 103 (40): 14767–72. doi:10.1073/pnas.0606877103. PMC 1578504. PMID 17003112.
• Coleman ML, McDonough MA, Hewitson KS, Coles C, Mecinovic J, Edelmann M, Cook KM, Cockman ME, Lancaster DE, Kessler BM, Oldham NJ, Ratcliffe PJ, Schofield CJ (2007). "Asparaginyl hydroxylation of the Notch ankyrin repeat domain by factor inhibiting hypoxia-inducible factor". J. Biol. Chem. 282 (33): 24027–38. doi:10.1074/jbc.M704102200. PMID 17573339.