4-Hydroxy-3-methylbut-2-enyl diphosphate reductase

4-Hydroxy-3-methylbut-2-enyl diphosphate reductase
Identifiers
EC number	1.17.1.2
CAS number	512789-14-9
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

4-Hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2, isopentenyl-diphosphate:NADP+ oxidoreductase, LytB, (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase, HMBPP reductase, IspH, LytB/IspH) is an enzyme in the non-mevalonate pathway. It acts upon (E)-4-Hydroxy-3-methyl-but-2-enyl pyrophosphate (or "HMB-PP").^{[1][2][3][4][5][6]}

(1) isopentenyl diphosphate + NAD(P)⁺ + H₂O $\rightleftharpoons$ (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H⁺

(2) dimethylallyl diphosphate + NAD(P)⁺ + H₂O $\rightleftharpoons$ (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H⁺

4-Hydroxy-3-methylbut-2-enyl diphosphate reductase is an iron-sulfur protein that contains either a [3Fe-4S] or a [4Fe-4S] cluster.

References

1. Rohdich, F., Hecht, S., Gärtner, K., Adam, P., Krieger, C., Amslinger, S., Arigoni, D., Bacher, A. and Eisenreich, W. (2002). "Studies on the nonmevalonate terpene biosynthetic pathway: Metabolic role of IspH (LytB) protein". Proc. Natl. Acad. Sci. USA 99: 1158–1163. PMID 11818558.
2. Hintz, M., Reichenberg, A., Altincicek, B., Bahr, U., Gschwind, R.M., Kollas, A.-K., Beck, E., Wiesner, J., Eberl, M. and Jomaa, H. (2001). "Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human T cells in Escherichia coli". FEBS Lett. 509: 317–322. PMID 11741609.
3. Charon, L., Pale-Grosdemange, C. and Rohmer, M. (1999). "On the reduction steps in the mevalonate independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis in the bacterium Zymomonas mobilis". Tetrahedron Lett. 40: 7231–7234.
4. Röhrich, R.C., Englert, N., Troschke, K., Reichenberg, A., Hintz, M., Seeber, F., Balconi, E., Aliverti, A., Zanetti, G., Köhler, U., Pfeiffer, M., Beck, E., Jomaa, H. and Wiesner, J. (2005). "Reconstitution of an apicoplast-localised electron transfer pathway involved in the isoprenoid biosynthesis of Plasmodium falciparum". FEBS Lett. 579: 6433–6438. PMID 16289098.
5. Wolff, M., Seemann, M., Bui, T.S.B., Frapart, Y., Tritsch, D., Garcia Estrabot, A., Rodríguez-Concepción, M., Boronat, A., Marquet, A. and Rohmer, M. (2003). "Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein". FEBS Lett. 541: 115–120. PMID 12706830.
6. Gräwert, T., Kaiser, J., Zepeck, F., Laupitz, R., Hecht, S., Amslinger, S., Schramek, N., Schleicher, E., Weber, S., Haslbeck, M., Buchner, J., Rieder, C., Arigoni, D., Bacher, A., Eisenreich, W. and Rohdich, F. (2004). "IspH protein of Escherichia coli: studies on iron-sulfur cluster implementation and catalysis". J. Am. Chem. Soc. 126: 12847–12855. PMID 15469281.