IAEDANS

IAEDANS
Names

IUPAC name 5-[2-[(2-Iodo-1-oxoethyl)amino]ethylamino]-1-naphthalenesulfonic acid
Identifiers
CAS Number	36930-63-9^Y
ChEBI	CHEBI:58984^Y
ChemSpider	82727^Y
Jmol interactive 3D	Image
PubChem	91621
InChI InChI=1S/C14H15IN2O4S/c15-9-14(18)17-8-7-16-12-5-1-4-11-10(12)3-2-6-13(11)22(19,20)21/h1-6,16H,7-9H2,(H,17,18)(H,19,20,21)^Y Key: ZMERMCRYYFRELX-UHFFFAOYSA-N^Y InChI=1/C14H15IN2O4S/c15-9-14(18)17-8-7-16-12-5-1-4-11-10(12)3-2-6-13(11)22(19,20)21/h1-6,16H,7-9H2,(H,17,18)(H,19,20,21) Key: ZMERMCRYYFRELX-UHFFFAOYAI
SMILES O=S(=O)(O)c1cccc2c1cccc2NCCNC(=O)CI
Properties
Chemical formula	C₁₄H₁₅IN₂O₄S
Molar mass	434.25 g/mol
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Y verify (what is ^YN ?)
Infobox references

IAEDANS is an organic fluorophore (fluorescent molecule). It stands for 5-({2-[(iodoacetyl)amino]ethyl}amino)naphthalene-1-sulfonic acid. It is widely used as a marker in fluorescence spectroscopy.

1,5-IAEDANS has a peak excitation wavelength of 336 nm and a peak emission wavelength of 490 nm. The extinction coefficient of the dye is 5700. It is soluble in dimethylformamide (DMF) or buffer above pH 6 and reacts primarily with thiols.

The absorption spectrum IAEDANS overlaps well with the emission spectrum of tryptophan, making it useful as an acceptor in FRET experiments. It can also be used as a resonance energy donor to fluorophores such as fluorescein, Alexa Fluor 488, Oregon Green, and BODIPY FL.

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