Immunomagnetic separation

Immunomagnetic separation (IMS) is a laboratory tool that can efficiently isolate cells out of body fluid or cultured cells. It can also be used as a method of quantifying the pathogenicity of food, blood or feces. DNA analysis have supported the combined use of both this technique and Polymerase Chain Reaction (PCR).[1] Another laboratory separation tool is the affinity magnetic separation (AMS), which is more suitable for the isolation of prokaryotic cells.[2]

Technique

Antibodies coating paramagnetic beads will bind to antigens present on the surface of cells thus capturing the cells and facilitate the concentration of these bead-attached cells. The concentration process is created by a magnet placed on the side of the test tube bringing the beads to it.

References

  1. ↑ Engstrand, L. and Enroth, H., Journal of Clinical microbiology, vol.33, no.8, August 1995, p. 2162-2165.
  2. ↑ Affinity magnetic separation of Listeria spp and Escherichia coli O157 (Bacteria Capture Kit)

Source


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