Isochorismatase

isochorismatase
Identifiers
EC number	3.3.2.1
CAS number	37288-64-5
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, an isochorismatase (EC 3.3.2.1) is an enzyme that catalyzes the chemical reaction

isochorismate + H₂O $\rightleftharpoons$ 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate

Thus, the two substrates of this enzyme are isochorismate and H₂O, whereas its two products are 2,3-dihydroxy-2,3-dihydrobenzoate and pyruvate.

This enzyme belongs to the family of hydrolases, specifically those acting on ether bonds (ether hydrolases). The systematic name of this enzyme class is isochorismate pyruvate-hydrolase. Other names in common use include 2,3-dihydro-2,3-dihydroxybenzoate synthase, 2,3-dihydroxy-2,3-dihydrobenzoate synthase, and 2,3-dihydroxy-2,3-dihydrobenzoic synthase. This enzyme participates in the biosynthesis of siderophore group (nonribosomal).

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1NF8, 1NF9, and 2FQ1.

References

• Young IG, Gibson F (1969). "Regulation of the enzymes involved in the biosynthesis of 2,3-dihydroxybenzoic acid in Aerobacter aerogenes and Escherichia coli". Biochim. Biophys. Acta 177 (3): 401–11. doi:10.1016/0304-4165(69)90302-x. PMID 4306838.