Juvenile hormone epoxide hydrolase

Juvenile hormone epoxide hydrolase

Crystal structure of juvenile hormone epoxide hydrolase from the silkworm Bombyx mori.[1]
Identifiers
Organism Bombyx mori
Symbol JHEH
Alt. symbols Jheh2
Entrez 692686
PDB 4QLA
RefSeq (mRNA) NM_001043736.1
RefSeq (Prot) NP_001037201.1
UniProt Q6U6J0
Other data
EC number 3.3.2.9
Chromosome shotgun: 0.63 - 0.65 Mb

Juvenile hormone epoxide hydrolase (JHEH) is an enzyme that inactivates insect juvenile hormones. This inactivation is accomplished through hydrolysis of the epoxide functional group contained within these hormones into diols. JHEH is one of two enzymes involved in the termination of signaling properties of the various juvenile hormones.[2] The other is juvenile-hormone esterase, or JH.[2]

The first observation of activity from JHEH was in Rhodnius prolixus and Schistocerca gregaria. JH esterase was also first observed in this same work.[2]

Mechanism

JH esterase and epoxide hydrolase are the only known enzymes involved in primary degradation of JH. The inactivated carboxylic acid products of juvenile hormone esterase can be reactivated in peripheral tissues by esterification restoring its biological activity. In contrast, the juvenile hormone diols that are the product of the epoxide hydrolase are very hydrophilic and cannot be converted back to JH. Thus, this enzyme permantly terminates the action of juvenile hormone, unlike JH esterase.

The enzyme mechanism involves the addition of water at the secondaary carbon, C-10.[3] In Trichoplusia ni, JH has been found to be the preferred substrate of this enzyme as compared with JH acid.[4][5]

Isoforms

Two forms of JHEH have been cloned from Leptinotarsa decemlineata, which are found in all tissues studied. Knockdown of these enzymes by injected appropriate RNA interference has been found to increase JH titers, prolong laval development, and delay adult emergence, all symptoms of excessive levels of JH.[5]

Structure

JHEH is a membrane associated protein, and by photoaffinity labeling has been shown to be a 50 kDA protein in Manduca sexta.[6]

It has been noted by several that properties of JHEH are similar to those of animal microsomal epoxide hydrolase. Sequence alignments showed that the exact catalytic triad of the animal enzyme (Asp-226, Glu-403 and His-430) is present in JHEH.[7] In addition, the X-ray structure of Bombyx mori JHEH was recently determined.[1]

Post translational modification

Juvenile hormone diol is acted on by juvenile hormone diol kinase, to give juvenile hormone diol phosphate, with the phosphate attached to the hydroxyl group on carbon atom 10. This modication greatly enhaces the water solubiity, making it easier to excrete.

References

  1. 1 2 PDB: 4QLA​; Zhou K, Jia N, Hu C, Jiang YL, Yang JP, Chen Y, Li S, Li WF, Zhou CZ (2014). "Crystal structure of juvenile hormone epoxide hydrolase from the silkworm Bombyx mori". Proteins 82 (11): 3224–9. doi:10.1002/prot.24676. PMID 25143157.
  2. 1 2 3 White AF (February 1972). "Metabolism of the juvenile hormone analogue methyl farnesoate 10, 11-epoxide in two insect species". Life Sciences 11 (4): 201–210. doi:10.1016/0024-3205(72)90110-5.
  3. ↑ Roe RM, Kallapur V, Linderman RJ, Viviani F, Harris SV, Walker EA, Thompson DM (1996). "Mechanism of action and cloning of epoxide hydrolase from the cabbage looper, Trichoplusia ni". Arch. Insect Biochem. Physiol. 32 (3-4): 527–35. doi:10.1002/(SICI)1520-6327(1996)32:3/4<527::AID-ARCH24>3.0.CO;2-D. PMID 8756307.
  4. ↑ Kallapur VL, Majumder C, Roe RM (February 1996). "In vivo and in vitro-tissue specific metabolism of juvenile hormone during the last stadium of the cabbage looper, Trichoplusia ni". Journal of Insect Physiology 42 (2): 181–190. doi:10.1016/0022-1910(95)00057-7.
  5. 1 2 Lü FG, Fu KY, Guo WC, Li GQ (2015). "Characterization of two juvenile hormone epoxide hydrolases by RNA interference in the Colorado potato beetle". Gene 570 (2): 264–71. doi:10.1016/j.gene.2015.06.032. PMID 26079572.
  6. ↑ Touhara K, Soroker V, Prestwich GD (June 1994). "Photoaffinity labeling of juvenile hormone epoxide hydrolase and JH-binding proteins during ovarian and egg development in Manduca sexta". Insect Biochemistry and Molecular Biology 24 (6): 633–640. doi:10.1016/0965-1748(94)90100-7.
  7. ↑ Harris SV, Thompson DM, Linderman RJ, Tomalski MD, Roe RM (1999). "Cloning and expression of a novel juvenile hormone-metabolizing epoxide hydrolase during larval-pupal metamorphosis of the cabbage looper, Trichoplusia ni". Insect Mol. Biol. 8 (1): 85–96. doi:10.1046/j.1365-2583.1999.810085.x. PMID 9927177.
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