KAHRP

KAHRP (Plasmodium falciparum knob-associated histidine-rich protein) is a protein expressed in erythrocytes infected by Plasmodium falciparum. KAHRP is a major component of knobs, feature found on Plasmodium falciparum infected erythrocytes.

It has been suggested that KAHRP may play a role in trafficking or docking PfEMP1, major malarial cytoadherence protein to the erythrocyte membrane;[1] however, these findings were disputed by recent NMR and fluorescence anisotropy studies showing no interaction between PfEMP1 and KAHRP.[2]

Instead, KAHRP was shown to interact with Ankyrin, more precisely the D3 subunit of the Membrane-binding domain of Ankyrin type 1.[3] This interaction was suggested via SPR, ELISA, and Pulldown studies, however, it has not been confirmed by NMR, ITC, crystallography, or fluorescence anisotropy.

References

  1. ↑ Alexander G. Maier, Brian M. Cooke, Alan F. Cowman & Leann Tilley (May 2009). "Malaria parasite proteins that remodel the host erythrocyte : Abstract". Nature Reviews Microbiology 7 (5): 341–354. doi:10.1038/nrmicro2110. Retrieved 2014-03-15.
  2. ↑ Christina Mayer, Leanne Slater,Michele C. Erat, Robert Konrat and Ioannis Vakonakis (March 2, 2012). "Structural Analysis of the Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) Intracellular Domain Reveals a Conserved Interaction Epitope". J Biol Chem. 287(10): 7182–7189. doi:10.1074/jbc.M111.330779. PMC 3293552. PMID 22249178.
  3. ↑ Weng H1, Guo X, Papoin J, Wang J, Coppel R, Mohandas N, An X. (2014-01-24). "Interaction of Plasmodium falciparum knob-associated histidine-rich protein (KAHRP) with erythrocyte ankyrin R is required for its attachment to the erythrocyte membrane.". Biochim Biophys Acta 1838 (1 Pt B): 185–192. doi:10.1016/j.bbamem.2013.09.014. PMID 24090929.


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