Lecythophora hoffmannii

Lecythophora hoffmannii
Scientific classification
Kingdom: Fungi
Division: Ascomycota
Class: Sordariomycetes
Order: Coniochaetales
Family: Coniochaetaceae
Genus: Lecythophora
Species: L. hoffmannii
Binomial name
Lecythophora hoffmannii
(J.F.H.Beyma) W.Gams & McGinnis (1983)
Synonyms
  • Phialophora hoffmannii (J.F.H.Beyma) Schol-Schwarz (1970)
  • Margarinomyces hoffmannii J.F.H.Beyma (1938)

Lecythophora hoffmannii, also known as Phialophora hoffmannii, is an ascomycete fungus that grows commonly in soil.[1] It has also been categorized as a soft-rot fungus capable of bringing the surface layer of timber into a state of decay, even when safeguarded with preservatives.[2] Additionally, it has pathogenic properties, although it causes serious infection only in rare cases.[3] A plant pathogen lacking a known sexual state,[4] L. hoffmannii has been classified as a "dematiaceous fungus" despite its contradictory lack of pigmentation; both in vivo and in vitro, there is no correlation between its appearance and its classification.[3]

History

Lecythophora hoffmannii was originally described by Beyma in 1939 as Margarinomyces hoffmannii.[5] The genus Lecythophora, originally described by Nannfeldt in 1934, was reintroduced by Gams and McGinnis to accommodate this species in 1983.[6]

Ecology

Occurrence

Lecythophora hoffmannii is a plant pathogen that commonly inhabits fertile soil.[2] It is also known as an agent of soft-rot wood in terrestrial and aquatic environments, it is known to colonize wood surfaces through the use of soft rot hyphae.[7]

Environmental Interactions

In soil,[1] L. hoffmannii is thought primarily to be a decomposer of wood. To this end, L. hoffmannii is one of, if not the most important fungal agents of soft rot in preservative-treated wood. It, along with soft rot species of other genera, is known to metabolize aromatic compounds of low molecular weight; this includes nine phenolic compounds metabolized by L. hoffmannii itself, such as p-hydroxybenzoic acid, vanillic acid, and vanillin, to name a few. This allows for the utilization of part of the amorphous granular material, found in said phenolic compounds, which contains the lignin breakdown products produced in soft-rot activities.[2]

Decay of wood by soft rot fungi such as L. hoffmannii occurs in one of two ways: either the enzymes released from the hyphae on the lumen surface of the wood cell wall erode it, or the cavities around the hyphae in the S2region of the cell wall get excavated. The cavity itself is created by the enzymatic activity along the length of the hyphae after its growth.[7]

Morphology

As a member of the genus Lecythophora, it is considered to be poorly differentiated morphologically.[8] Lecythophora hoffmannii colonies range in colour from a pale salmon to a pallid orange, with degenerate strains presenting a creamy white colour.[9] Colonies are also flat, smooth and moist.[10] Collarettes are distinct,[9] yet unpigmented, while conidia are also hyaline, smooth, and thin-walled.[10] The production of slimy, orange to salmon-colored colonies on short adelophialides with cylindrical collarettes can be quite distinct, with ventricose phialide formations less frequent. Narrow and hyaline, hyphae, through the use of small collarettes,[10] produce conidia directly upon them laterally, directly upon the vegetative hyphae.[8] Alternatively, flask-shaped lateral cells, which are practically cylindrical, can serve as the medium through which conidia are produced; they are sometimes found to be arranged in densely packed groups.[10] Conidia are slightly curved, appearing broadly ellipsoidal to cylindrical to bean-shaped;[8] measurements vary from 3.5–7.0 µm in length by 1.0–2.5 µm in width,[9] to 3.0–3.5 µm by 1.5–2.5 µm.[10]

Ultrastructure

There are multiple types of hyphae within L. hoffmannii. Ones to note are fine soft rot hyphae, T-branch hyphae and proboscis hyphae. T-branch hyphae, which penetrate the wood cell wall, arise from lumen-colonizing hyphae. During early stages of their development, T-branch hyphae contain few cellular organelles; later on in that development, the T-branch arms elongate, complexifying the internal organization of the hyphae. The tip of the T-branch is lacking in hyphal distortion, as well.[7] Proboscis hyphae, when young, exhibit a similar ultrastructural organization and overall diameter to young T-branch hyphae upon emergence from the cone region of parent cavities. They are also fine (only 0.24 µm in diameter (0.34 µm diam including the fungal cell wall) with the tips showing an electron-dense zone.[7] The occurrence of membrane configurations at the base of fine hyphae within proboscis hyphae has importance when it comes to the transport and release of extracellular lytic enzymes.[11] Hyphal diameter is measured to be 0.87 to 1.82 µm.[7]

Physiology

Intolerant to benomyl,[12] L. hoffmannii is capable of proteolytic activity (hydrolysis, in particular), with proteolytic enzymes detected in cultures of L. hoffmannii with various media, including Loeffler blood serum, and skim milk and Litmus milk.[13] Lecythophora hoffmannii is also quite thermotolerant, easily growing at 30 and 35 °C, with two out of four isolates (both environmental) growing at 40 °C.[13]

Disease

Despite being one of only two species of medical interest within the genus Lecythophora,[10] Lecythophora hoffmannii is rarely encountered as a pathogen primarily due to its related taxa. It is one of the more predominant species of its genus concerning the number of clinical cases reporting infection, having been associated with cases of subcutaneous infections, keratitis, sinusitis, and peritonitis.[8] Lecythophora hoffmannii rarely causes disease, but when it does, it is only seen in immunocompromised patients, thus marking it as an opportunistic fungal pathogen.[3] Other identifying factors for opportunistic pathogens like this include an association with other underlying diseases capable of compromising the host's immune system, an association with new therapeutic modalities, and cases of invasive fungal sinusitis with no determinable predisposing factors. While great advances in medical technology have decreased previously high rates of morbidity and mortality, the amount and variation of opportunistic pathogens has also increased.[14]

L. hoffmannii has been inculpated in human phaeohyphomycosis, leading to the aforementioned abscesses, sinusitis, and mastoiditis, to name a few.[15] It has also been implicated as a food contaminant, which could explain how people or animals interact with it in their respective environments.[16]

Human

In rare cases, L. hoffmannii has demonstrated its ability to cause serious infection; in one case, it actually induced chronic sinusitis in an AIDS patient. The patient in question was immunocompromised and thus severely immunodeficient due to contracting the human immunodeficiency virus; this allowed for the fungal pathogen to not only infect the patient (where an intact immune system would have made an infection by L. hoffmannii a non-issue), but inflict a recurrent, chronic disease such as sinusitis.[3]

Lecythophora hoffmannii has also been isolated as a pure growth fungal aggregate from a human gluteal abscess; it actually develops an aggregate not only after treatment is attempted (in this case, for a respiratory infection, where various anti-bacterial drug treatments were implemented), but at the exact point of inoculation,[5] once again demonstrating its immunity to anti-fungal agents and treatment methods.

Non-human

There are parallels in both humans and animals when it comes to infections caused by L. hoffmannii. In humans, L. hoffmannii is not only the causative agent when it comes to original infection, but it also exacerbates the symptoms upon administration of anti-fungal treatments. In the case of a household dog suffering from claudication in the jaw, rigorous treatment with ketoconazole and itraconazole (for 3 total times per day over 3 months) had no effect, since this fungus is resistant to such treatments.[16]

Treatment

Lecythophora hoffmannii has proven to be resistant to multiple anti-fungal agents, including amphotericin B, flucytosine, ketoconazole, and fluconazole.[3] Because of this, precautions have been taken, such as antifungal susceptibility testing, in order to circumvent such drawbacks. Through this method, polyhexamethylene biguanide (PHMB) has been identified and utilized in conjunction with invasive surgical procedures to successfully treat one of the only cases of infection at the hands of this fungus. This method of treatment is employed in order to safeguard against fungal infection even in an immunocompetent host.[14]

References

  1. 1 2 Domsch, K.H; W. Gams ; T.H. Anderson (1993). Compendium of Soil Fungi (Reprint [der Ausg. London] 1980. ed.). Eching: IHW-Verl. ISBN 3980308383.
  2. 1 2 3 Bugos, RC; Sutherland, JB; Adler, JH (July 1988). "Phenolic Compound Utilization by the Soft Rot Fungus Lecythophora hoffmannii.". Applied and Environmental Microbiology 54 (7): 1882–5. PMID 16347701.
  3. 1 2 3 4 5 Marriott, DJ; Wong, KH; Aznar, E; Harkness, JL; Cooper, DA; Muir, D (November 1997). "Scytalidium dimidiatum and Lecythophora hoffmannii: unusual causes of fungal infections in a patient with AIDS.". Journal of clinical microbiology 35 (11): 2949–52. PMID 9350765.
  4. ↑ "Synonym and Classification Data for Lecythophora". Doctor Fungus. Retrieved 18 October 2013.
  5. 1 2 Rinaldi, MG; McCoy, EL; Winn, DF (July 1982). "Gluteal abscess caused by Phialophora hoffmannii and review of the role of this organism in human mycoses.". Journal of clinical microbiology 16 (1): 181–5. PMID 7107854.
  6. ↑ Damm, U.; Fourie, P.H.; Crous, P.W. (18 June 2010). "Coniochaeta (Lecythophora), Collophora gen. nov. and Phaeomoniella species associated with wood necroses of Prunus trees". Persoonia - Molecular Phylogeny and Evolution of Fungi 24 (1): 60–80. doi:10.3767/003158510X500705. PMID 20664761.
  7. 1 2 3 4 5 Hale, Michael D.; Eaton, Rodney A. (May–June 1985). "The Ultrastructure of Soft Rot Fungi. I. Fine Hyphae in Wood Cell Walls". Mycologia 77 (3): 447–463. doi:10.2307/3793202. JSTOR 3793202.
  8. 1 2 3 4 Perdomo, H.; Sutton, DA; García, D; Fothergill, AW; Gené, J; Cano, J; Summerbell, RC; Rinaldi, MG; Guarro, J (April 2011). "Molecular and phenotypic characterization of Phialemonium and Lecythophora isolates from clinical samples.". Journal of clinical microbiology 49 (4): 1209–16. doi:10.1128/jcm.01979-10. PMID 21270235.
  9. 1 2 3 Howard, Dexter H. (2003). Pathogenic fungi in humans and animals (2. ed.). New York [u.a.]: Dekker. ISBN 0824706838.
  10. 1 2 3 4 5 6 "Lecythophora hoffmannii". Mycology Online. University of Adelaide. Retrieved 18 October 2013.
  11. ↑ Hale, Michael D.; Eaton, Rodney A. (Jul–Aug 1985). "The Ultrastructure of Soft Rot Fungi. II. Cavity-Forming Hyphae in Wood Cell Walls". Mycologia 77 (4): 594–605. doi:10.2307/3793358. JSTOR 3793358.
  12. ↑ "Lecythophora hoffmannii". MycoBank. Retrieved 18 October 2013.
  13. 1 2 Espinel-Ingroff, A; Goldson, PR; McGinnis, MR; Kerkering, TM (February 1988). "Evaluation of proteolytic activity to differentiate some dematiaceous fungi.". Journal of clinical microbiology 26 (2): 301–7. PMID 3343325.
  14. 1 2 Chang, Christopher Y.; Schell, Wiley A.; Perfect, John R.; Hulka, Gregory F. (1 June 2005). "Novel Use of a Swimming Pool Biocide in the Treatment of a Rare Fungal Mastoiditis". The Laryngoscope 115 (6): 1065–1069. doi:10.1097/01.MLG.0000163338.45700.FE.
  15. ↑ Liu, Dongyou. Molecular detection of human fungal pathogens. Boca Raton, Fla.: CRC Press. ISBN 1439812403.
  16. 1 2 Sakaeyama, S; Sano, A; Murata, Y; Kamei, K; Nishimura, K; Hatai, K (May 2007). "Lecythophora hoffmannii isolated from a case of canine osteomyelitis in Japan.". Medical mycology : official publication of the International Society for Human and Animal Mycology 45 (3): 267–72. doi:10.1080/13693780601188602. PMID 17464847.
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