Linoleate 8R-lipoxygenase

Linoleate 8R-lipoxygenase (EC 1.13.11.60, linoleic acid 8R-dioxygenase, 5,8-LDS (bifunctional enzyme), 7,8-LDS (bifunctional enzyme), 5,8-linoleate diol synthase (bifunctional enzyme), 7,8-linoleate diol synthase (bifunctional enzyme), PpoA) is an enzyme with systematic name linoleate:oxygen (8R)-oxidoreductase.^[1]^[2]^[3]^[4] This enzyme catalyses the following chemical reaction

linoleate + O₂

\rightleftharpoons

(8R,9Z,12Z)-8-hydroperoxyoctadeca-9,12-dienoate

Linoleate 8R-lipoxygenase contains heme.

References

↑ Brodhun, F., Gobel, C., Hornung, E. and Feussner, I. (2009). "Identification of PpoA from Aspergillus nidulans as a fusion protein of a fatty acid heme dioxygenase/peroxidase and a cytochrome P₄₅₀". J. Biol. Chem. 284 (18): 11792–11805. doi:10.1074/jbc.M809152200. PMC 2673248. PMID 19286665.
↑ Hamberg, M., Zhang, L.-Y., Brodowsky, I.D. and Oliw, E.H. (1994). "Sequential oxygenation of linoleic acid in the fungus Gaeumannomyces graminis: stereochemistry of dioxygenase and hydroperoxide isomerase reactions". Arch. Biochem. Biophys. 309: 77–80. doi:10.1006/abbi.1994.1087. PMID 8117115.
↑ Garscha, U. and Oliw, E. (2008). "Pichia expression and mutagenesis of 7,8-linoleate diol synthase change the dioxygenase and hydroperoxide isomerase". Biochem. Biophys. Res. Commun. 373 (4): 579–583. doi:10.1016/j.bbrc.2008.06.060. PMID 18586008.
↑ Su, C. and Oliw, E.H. (1996). "Purification and characterization of linoleate 8-dioxygenase from the fungus Gaeumannomyces graminis as a novel hemoprotein". J. Biol. Chem. 271: 14112–14118. doi:10.1074/jbc.271.24.14112. PMID 8662736.

External links

Linoleate 8R-lipoxygenase at the US National Library of Medicine Medical Subject Headings (MeSH)

Oxidoreductases: monooxygenases (EC 1.13)

1.13.11: two atoms of oxygen	lipoxygenase: Arachidonate 5-lipoxygenase Arachidonate 12-lipoxygenase/ALOX12 Arachidonate 8-lipoxygenase Arachidonate 15-lipoxygenase/ALOX15 Linoleate 11-lipoxygenase other dioxygenase: Catechol dioxygenase Homogentisate 1,2-dioxygenase Cysteine dioxygenase 4-Hydroxyphenylpyruvate dioxygenase Indoleamine 2,3-dioxygenase Chlorite dismutase

1.13.12: one atom of oxygen	Firefly luciferase

1.13.99: other	Inositol oxygenase

Enzymes

Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Catalytically perfect enzyme Coenzyme Cofactor Enzyme catalysis Enzyme kinetics Lineweaver–Burk plot Michaelis–Menten kinetics

Regulation	Allosteric regulation Cooperativity Enzyme inhibitor

Classification	EC number Enzyme superfamily Enzyme family List of enzymes

Types	EC1 Oxidoreductases(list) EC2 Transferases(list) EC3 Hydrolases(list) EC4 Lyases(list) EC5 Isomerases(list) EC6 Ligases(list)

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