| State | Unit name | Species of concern | Unit area (acres) |
| Alabama | Sauta Cave NWR | Indiana bat, gray bat | 264 |
| Fern Cave NWR | Indiana bat, gray bat | 199 |
| Key Cave NWR | Alabama cavefish, gray bat | 1,060 |
| Watercress Darter NWR | Watercress darter | 25 |
| Arizona | Buenos Aires NWR | Masked bobwhite quail | 116,585 |
| Leslie Canyon NWR | Gila topminnow, Yaqui chub, peregrine falcon | 2,765 |
| San Bernardino NWR | Gila topminnow, Yaqui chub, Yaqui catfish, beautiful shiner, Huachuca water umbel | 2,369 |
| Arkansas | Logan Cave NWR | Cave crayfish, gray bat, Indiana bat, Ozark cavefish | 124 |
| California | Antioch Dunes NWR | Lange's metalmark butterfly, Antioch Dunes evening-primrose, Contra Costa wallflower | 55 |
| Bitter Creek NWR | California condor | 14,054 |
| Blue Ridge NWR | California condor | 897 |
| Castle Rock NWR | Aleutian Canada goose | 14 |
| Coachella Valley NWR | Coachella Valley fringe-toed lizard | 3,592 |
| Don Edwards San Francisco Bay NWR | California clapper rail, California least tern, salt marsh harvest mouse | 21,524 |
| Ellicott Slough NWR | Santa Cruz long-toed salamander | 139 |
| Hopper Mountain NWR | California condor | 2,471 |
| Sacramento River NWR | Valley elderberry longhorn beetle, bald eagle, least Bell's vireo | 7,884 |
| San Diego NWR | San Diego fairy shrimp, San Diego mesa mint, Otay mesa mint, California Orcutt grass, San Diego button-celery | 1,840 |
| San Joaquin River NWR | Aleutian Canada goose | 1,638 |
| Seal Beach NWR | Light-footed clapper rail, California least tern | 911 |
| | Sweetwater Marsh NWR | Light-footed clapper rail | 316 |
| Tijuana Slough NWR | Light-footed clapper rail | 1,023 |
| Florida | Archie Carr NWR | Loggerhead sea turtle, green sea turtle | 29 |
| Crocodile Lake NWR | American crocodile | 6,686 |
| Crystal River NWR | West Indian manatee | 80 |
| Florida Panther NWR | Florida panther | 23,379 |
| Hobe Sound NWR | Loggerhead sea turtle, green sea turtle | 980 |
| Lake Wales Ridge NWR | Florida scrub jay, snakeroot, scrub blazing star, Carter's mustard, papery Whitlow-wort, Florida bonamia, scrub lupine, highlands scrub hypericum, Garrett's mint, scrub mint, pygmy fringe-tree, wireweed, Florida ziziphus, scrub plum, eastern indigo snake, bluetail mole skink, sand skink | 659 |
| National Key Deer Refuge | Key deer | 8,542 |
| St. Johns NWR | Dusky seaside sparrow | 6,255 |
| Hawaii | Hakalau Forest NWR | Akepa, akiapolaau, 'o'u, Hawaiian hawk, Hawaiian creeper | 32,730 |
| Hanalei NWR | Hawaiian stilt, Hawaiian coot, Hawaiian gallinule, Hawaiian duck | 917 |
| Huleia NWR | Hawaiian stilt, Hawaiian coot, Hawaiian gallinule, Hawaiian duck | 241 |
| James C. Campbell NWR | Hawaiian stilt, Hawaiian coot, Hawaiian gallinule, Hawaiian duck | 164 |
| Kakahaia NWR | Hawaiian stilt, Hawaiian coot | 45 |
| Kealia Pond NWR | Hawaiian stilt, Hawaiian coot | 691 |
| Pearl Harbor NWR | Hawaiian stilt | 61 |
| Iowa | Driftless Area NWR | Iowa Pleistocene snail | 521 |
| Massachusetts | Massasoit NWR | Plymouth red-bellied turtle | 184 |
| Michigan | Kirtlands Warbler Wildlife Management Area | Kirtland's warbler | 6,535 |
| Mississippi | Mississippi Sandhill Crane NWR | Mississippi sandhill crane | 19,713 |
| Missouri | Ozark Cavefish NWR | Ozark cavefish | 42 |
| Pilot Knob NWR | Indiana bat | 90 |
| Montana | Red Rock LakesNWR | Trumpeter swan | 65,810 |
| Nebraska | Karl E. Mundt NWR | Bald eagle | 19 |
| Nevada | Ash Meadows NWR | Devil's Hole pupfish, Warm Springs pupfish, Ash Meadows Amargosa pupfish, Ash Meadows speckled dace, Ash Meadows naucorid, Ash Meadows blazing star, Amargosa niterwort, Ash Meadows milk-vetch, Ash Meadows sunray, spring-loving centaury, Ash Meadows gumplant, Ash Meadows ivesia | 13,268 |
| Moapa Valley NWR | Moapa dace | 32 |
| Oklahoma | Ozark Plateau NWR | Ozark big-eared bat, gray bat | 2,208 |
| Oregon | Bear Valley NWR | Bald eagle | 4,200 |
| Julia Butler Hansen Refuge for the Columbian White-tail Deer | Columbian white-tailed deer | 2,750 |
| Nestucca Bay NWR | Aleutian Canada goose | 457 |
| South Dakota | Karl E. Mundt NWR | Bald eagle | 1,044 |
| Texas | Attwater Prairie Chicken NWR | Attwater's greater prairie chicken | 8,007 |
| Balcones Canyonlands NWR | Black-capped vireo, golden-cheeked warbler | 14,144 |
| US Virgin Islands | Green Cay NWR | St. Croix ground lizard | 14 |
| Sandy Point NWR | Leatherback sea turtle | 327 |
| Virginia | James River NWR | Bald eagle | 4,147 |
| Mason Neck NWR | Bald eagle | 2,276 |
| Washington | Julia Butler Hansen Refuge for the Columbian White-tail Deer | Columbian white-tailed deer | 2,777 |
| Wyoming | Mortenson Lake NWR | Wyoming toad | 1,776 |