| Name |
State(s) |
Acreage |
Area: km² |
| Arctic National Wildlife Refuge |
Alaska |
19,286,482.30 |
78,049.62 |
| Yukon Delta National Wildlife Refuge |
Alaska |
19,162,416.50 |
77,547.55 |
| Yukon Flats National Wildlife Refuge |
Alaska |
8,634,511.57 |
34,942.63 |
| Togiak National Wildlife Refuge |
Alaska |
4,102,537.38 |
16,602.38 |
| Innoko National Wildlife Refuge |
Alaska |
3,850,481.05 |
15,582.34 |
| Alaska Peninsula National Wildlife Refuge |
Alaska |
3,563,489.83 |
14,420.93 |
| Koyukuk National Wildlife Refuge |
Alaska |
3,550,160.50 |
14,366.99 |
| Alaska Maritime National Wildlife Refuge |
Alaska |
3,417,756.91 |
13,831.17 |
| Selawik National Wildlife Refuge |
Alaska |
2,150,161.99 |
8,701.40 |
| Kodiak National Wildlife Refuge |
Alaska |
1,990,418.11 |
8,054.94 |
| Kenai National Wildlife Refuge |
Alaska |
1,912,425.40 |
7,739.31 |
| Desert National Wildlife Refuge |
Nevada |
1,615,313.49 |
6,536.94 |
| Nowitna National Wildlife Refuge |
Alaska |
1,560,000.00 |
6,313.10 |
| Kanuti National Wildlife Refuge |
Alaska |
1,430,160.17 |
5,787.65 |
| Becharof National Wildlife Refuge |
Alaska |
1,200,059.57 |
4,856.47 |
| Charles M. Russell National Wildlife Refuge |
Montana |
915,294.22 |
3,704.06 |
| Cabeza Prieta National Wildlife Refuge |
Arizona |
860,041.32 |
3,480.46 |
| Tetlin National Wildlife Refuge |
Alaska |
700,058.54 |
2,833.04 |
| Kofa National Wildlife Refuge |
Arizona |
666,561.26 |
2,697.48 |
| Sheldon National Wildlife Refuge |
Nevada / Oregon |
573,503.63 |
2,320.89 |
| Okefenokee National Wildlife Refuge |
Georgia / Florida |
401,908.38 |
1,626.47 |
| Izembek National Wildlife Refuge |
Alaska |
311,075.78 |
1,258.88 |
| Hart Mountain National Antelope Refuge |
Oregon |
270,003.58 |
1,092.67 |
| Hawaiian Islands National Wildlife Refuge |
Hawaii |
254,418.10 |
1,029.59 |
| Sevilleta National Wildlife Refuge |
New Mexico |
229,673.57 |
929.46 |
| Key West National Wildlife Refuge |
Florida |
208,308.17 |
842.99 |
| Upper Mississippi River National Wildlife and Fish Refuge |
Wisconsin / Iowa / Minnesota / Illinois |
208,143.53 |
842.33 |
| Hanford Reach National Monument |
Washington |
195,719.00[2] |
702.05 |
| Malheur National Wildlife Refuge |
Oregon |
187,126.94 |
757.28 |
| White River National Wildlife Refuge |
Arkansas |
158,976.07 |
643.35 |
| Alligator River National Wildlife Refuge |
North Carolina |
153,017.15 |
619.24 |
| Arthur R. Marshall Loxahatchee National Wildlife Refuge |
Florida |
143,953.77 |
582.56 |
| Merritt Island National Wildlife Refuge |
Florida |
139,189.40 |
563.28 |
| Sabine National Wildlife Refuge |
Louisiana |
125,790.08 |
509.05 |
| Great White Heron National Wildlife Refuge |
Florida |
117,720.63 |
476.40 |
| Buenos Aires National Wildlife Refuge |
Arizona |
117,107.16 |
473.92 |
| Aransas National Wildlife Refuge |
Texas |
114,657.05 |
464.00 |
| Great Dismal Swamp National Wildlife Refuge |
Virginia / North Carolina |
111,202.81 |
450.02 |
| Pocosin Lakes National Wildlife Refuge |
North Carolina |
110,106.64 |
445.59 |
| Seney National Wildlife Refuge |
Michigan |
95,244.81 |
385.44 |
| Lower Rio Grande Valley National Wildlife Refuge |
Texas |
90,441.09 |
366.00 |
| Grasslands Wildlife Management Area |
California |
89,012.27 |
360.22 |
| Laguna Atascosa National Wildlife Refuge |
Texas |
88,388.69 |
357.70 |
| Stillwater National Wildlife Refuge |
Nevada |
87,271.26 |
353.17 |
| National Key Deer Refuge |
Florida |
84,824.28 |
343.27 |
| Baca National Wildlife Refuge |
Colorado |
78,696.65 |
318.47 |
| Bear River Migratory Bird Refuge |
Utah |
73,996.41 |
299.45 |
| Tensas River National Wildlife Refuge |
Louisiana |
73,169.36 |
296.11 |
| Valentine National Wildlife Refuge |
Nebraska |
73,098.09 |
295.82 |
| Saint Marks National Wildlife Refuge |
Florida |
69,196.75 |
280.03 |
| Cape Romain National Wildlife Refuge |
South Carolina |
66,287.18 |
268.25 |
| Cache River National Wildlife Refuge |
Arkansas |
66,164.82 |
267.76 |
| Red Rock Lakes National Wildlife Refuge |
Montana |
65,810.25 |
266.32 |
| Felsenthal National Wildlife Refuge |
Arkansas |
64,902.14 |
262.65 |
| Agassiz National Wildlife Refuge |
Minnesota |
61,500.93 |
248.89 |
| J. Clark Salyer National Wildlife Refuge |
North Dakota |
59,376.40 |
240.29 |
| Wichita Mountains Wildlife Refuge |
Oklahoma |
59,019.60 |
238.84 |
| McFaddin National Wildlife Refuge |
Texas |
58,861.43 |
238.20 |
| San Andres National Wildlife Refuge |
New Mexico |
57,215.48 |
231.54 |
| Bosque del Apache National Wildlife Refuge |
New Mexico |
57,191.10 |
231.44 |
| UL Bend National Wildlife Refuge |
Montana |
56,049.56 |
226.82 |
| Dakota Tallgrass Prairie Wildlife Management Area |
South Dakota / North Dakota |
55,850.29 |
226.02 |
| Tennessee National Wildlife Refuge |
Tennessee |
51,359.46 |
207.84 |
| Lower Suwannee National Wildlife Refuge |
Florida |
51,030.32 |
206.51 |
| Big Oaks National Wildlife Refuge |
Indiana |
51,000.00 |
206.39 |
| Lower Klamath National Wildlife Refuge |
California / Oregon |
50,912.68 |
206.04 |
| Mattamuskeet National Wildlife Refuge |
North Carolina |
50,173.93 |
203.05 |