M2 hol family

The Mycobacterial 2 TMS Phage Holin (M2 Hol) Family (TC# 1.E.36) is a group of transporters belonging to the Holin Superfamily VII.^[1] The Mycobactrerial 2 transmembrane segment (TMS) Holins have been identified and recognized by Catalao et al (2012).^[2] The Mycobacterium phage D29 gp11 protein (TC# 1.E.36.1.7) is a holin that, upon expression, rapidly kills both E. coli and Mycobacterium smegmatis. Shortening gp11 from its C-terminus resulted in diminished cytotoxicity and smaller holes. The two TMSs at the N-terminus alone do not integrate into the cytoplasmic membrane and do not show toxicity.^[3] Fusion of the two TMSs and a small C-terminal coiled-coil region resulted in restoration of cell killing. The second TMS is dispensable for toxicity. The gp11 C-terminal region is therefore necessary but not sufficient for toxicity.

See also

• Holin
• Lysin
• Transporter Classification Database

Further reading

• Delisle, Allan L.; Barcak, Gerard J.; Guo, Ming (2006-02-01). "Isolation and expression of the lysis genes of Actinomyces naeslundii phage Av-1". Applied and Environmental Microbiology 72 (2): 1110–1117.doi:10.1128/AEM.72.2.1110-1117.2006. ISSN 0099-2240.PMC 1392916. PMID 16461656.
• Lella, Muralikrishna; Mahalakshmi, Radhakrishnan (2013-10-25). "Pro-Gly mediated conformational switch of mycobacteriophage D29 holin transmembrane domain I is lipid concentration driven".Chemical Communications (Cambridge, England) 49 (83): 9594–9596. doi:10.1039/c3cc45058a. ISSN 1364-548X.PMID 24018990.

References

1. Reddy, Bhaskara L.; Saier Jr., Milton H. (2013-11-01). "Topological and phylogenetic analyses of bacterial holin families and superfamilies". Biochimica et Biophysica Acta (BBA) - Biomembranes 1828 (11): 2654–2671. doi:10.1016/j.bbamem.2013.07.004. PMC 3788059. PMID 23856191.
2. Catalão, Maria João; Gil, Filipa; Moniz-Pereira, José; São-José, Carlos; Pimentel, Madalena (2013-07-01). "Diversity in bacterial lysis systems: bacteriophages show the way". FEMS microbiology reviews 37 (4): 554–571. doi:10.1111/1574-6976.12006. ISSN 1574-6976. PMID 23043507.
3. Kamilla, Soumya; Jain, Vikas (2016-01-01). "Mycobacteriophage D29 holin C-terminal region functionally assists in holin aggregation and bacterial cell death". The FEBS journal 283 (1): 173–190. doi:10.1111/febs.13565. ISSN 1742-4658. PMID 26471254.

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