MAF1

MAF1 homolog, negative regulator of RNA polymerase III
Crystal Structure of Repressor of RNA polymerase III transcription MAF1. PDB entry 3nr5[1]
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 3NR5​
Identifiers
Symbols	MAF1 ; MGC20332; MGC31779; MGC39758
External IDs	OMIM: 610210 MGI: 1916127 HomoloGene: 49867 GeneCards: MAF1 Gene
Gene ontology Molecular function • RNA polymerase III core binding • RNA polymerase III type 1 promoter DNA binding • RNA polymerase III type 2 promoter DNA binding • RNA polymerase III type 3 promoter DNA binding • GABA receptor binding Cellular component • intracellular • nucleus • nucleoplasm • nucleolus • cytoplasm • centrosome • cytosol • plasma membrane • axon • dendrite • intracellular membrane-bounded organelle • perinuclear region of cytoplasm • inhibitory synapse Biological process • transcription, DNA-templated • negative regulation of transcription from RNA polymerase III promoter • transcription factor TFIIIB complex assembly Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	84232	68877
Ensembl	ENSG00000179632	ENSMUSG00000022553
UniProt	Q9H063	Q9D0U6
RefSeq (mRNA)	NM_032272	NM_001164607
RefSeq (protein)	NP_115648	NP_001158079
Location (UCSC)	Chr 8: 144.1 – 144.11 Mb	Chr 15: 76.35 – 76.35 Mb
PubMed search

Repressor of RNA polymerase III transcription MAF1 homolog is a protein that in humans is encoded by the MAF1 gene.^[2][3][4]

This gene encodes a protein that is homologous to Maf1, a Saccharomyces cerevisiae protein which is highly conserved in eukaryotic cells. S. cerevisiae Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses Pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression.^[4]

References

1. Vannini, A.; Ringel, R.; Kusser, A. G.; Berninghausen, O.; Kassavetis, G. A.; Cramer, P. (2010). "Molecular Basis of RNA Polymerase III Transcription Repression by Maf1". Cell 143 (1): 59–70. doi:10.1016/j.cell.2010.09.002. PMID 20887893.
2. Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Bocher M, Blocker H, Bauersachs S, Blum H, Lauber J, Dusterhoft A, Beyer A, Kohrer K, Strack N, Mewes HW, Ottenwalder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A (Mar 2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
3. Pluta K, Lefebvre O, Martin NC, Smagowicz WJ, Stanford DR, Ellis SR, Hopper AK, Sentenac A, Boguta M (Jul 2001). "Maf1p, a Negative Effector of RNA Polymerase III in Saccharomyces cerevisiae". Mol Cell Biol 21 (15): 5031–40. doi:10.1128/MCB.21.15.5031-5040.2001. PMC 87229. PMID 11438659.
4. "Entrez Gene: MAF1 MAF1 homolog (S. cerevisiae)".

Further reading

• Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.
• Dias Neto E, Correa RG, Verjovski-Almeida S, et al. (2000). "Shotgun sequencing of the human transcriptome with ORF expressed sequence tags". Proc. Natl. Acad. Sci. U.S.A. 97 (7): 3491–6. doi:10.1073/pnas.97.7.3491. PMC 16267. PMID 10737800.
• Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Upadhya R, Lee J, Willis IM (2003). "Maf1 is an essential mediator of diverse signals that repress RNA polymerase III transcription". Mol. Cell 10 (6): 1489–94. doi:10.1016/S1097-2765(02)00787-6. PMID 12504022.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.
• Johnson SS, Zhang C, Fromm J, et al. (2007). "Mammalian Maf1 is a negative regulator of transcription by all three nuclear RNA polymerases". Mol. Cell 26 (3): 367–79. doi:10.1016/j.molcel.2007.03.021. PMID 17499043.
• Rollins J, Veras I, Cabarcas S, et al. (2007). "Human Maf1 negatively regulates RNA Polymerase III transcription via the TFIIB family members Brf1 and Brf2". Int. J. Biol. Sci. 3 (5): 292–302. doi:10.7150/ijbs.3.292. PMC 1865091. PMID 17505538.