MAPRE1

Microtubule-associated protein, RP/EB family, member 1
PDB rendering based on 1pa7.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1PA7​, 1TXQ​, 1UEG​, 1VKA​, 1WU9​, 1YIB​, 1YIG​, 2HKQ​, 2HL3​, 2HL5​, 2QJZ​, 2R8U​, 3GJO​, 3MTU​, 3TQ7​
Identifiers
Symbols	MAPRE1 ; EB1
External IDs	OMIM: 603108 MGI: 891995 HomoloGene: 56129 GeneCards: MAPRE1 Gene
Gene ontology Molecular function • protein binding • protein C-terminus binding • poly(A) RNA binding • microtubule plus-end binding Cellular component • cytoplasm • Golgi apparatus • centrosome • spindle • cytosol • microtubule • cytoplasmic microtubule • cortical microtubule cytoskeleton • cell projection membrane • microtubule plus-end Biological process • G2/M transition of mitotic cell cycle • mitotic cell cycle • organelle organization • mitotic nuclear division • small GTPase mediated signal transduction • cell proliferation • negative regulation of microtubule polymerization • protein localization to microtubule • cell division • positive regulation of microtubule plus-end binding Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	22919	13589
Ensembl	ENSG00000101367	ENSMUSG00000027479
UniProt	Q15691	Q61166
RefSeq (mRNA)	NM_012325	NM_007896
RefSeq (protein)	NP_036457	NP_031922
Location (UCSC)	Chr 20: 32.82 – 32.85 Mb	Chr 2: 153.74 – 153.77 Mb
PubMed search

Microtubule-associated protein RP/EB family member 1 is a protein that in humans is encoded by the MAPRE1 gene.^[1][2][3][4]

Function

The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family.^[4]

Interactions

MAPRE1 has been shown to interact with TERF1.^[5]

References

1. Su LK, Burrell M, Hill DE, Gyuris J, Brent R, Wiltshire R, Trent J, Vogelstein B, Kinzler KW (Aug 1995). "APC binds to the novel protein EB1". Cancer Res 55 (14): 2972–7. PMID 7606712.
2. Berrueta L, Kraeft SK, Tirnauer JS, Schuyler SC, Chen LB, Hill DE, Pellman D, Bierer BE (Sep 1998). "The adenomatous polyposis coli-binding protein EB1 is associated with cytoplasmic and spindle microtubules". Proc Natl Acad Sci U S A 95 (18): 10596–601. doi:10.1073/pnas.95.18.10596. PMC 27940. PMID 9724749.
3. Nakamura M, Zhou XZ, Lu KP (Jul 2001). "Critical role for the EB1 and APC interaction in the regulation of microtubule polymerization". Curr Biol 11 (13): 1062–7. doi:10.1016/S0960-9822(01)00297-4. PMID 11470413.
4. "Entrez Gene: MAPRE1 microtubule-associated protein, RP/EB family, member 1".
5. Nakamura M, Zhou XZ, Kishi S, Lu KP (Mar 2002). "Involvement of the telomeric protein Pin2/TRF1 in the regulation of the mitotic spindle". FEBS Lett. 514 (2-3): 193–8. doi:10.1016/S0014-5793(02)02363-3. PMID 11943150.

Further reading

• Tirnauer JS, Bierer BE (2000). "EB1 proteins regulate microtubule dynamics, cell polarity, and chromosome stability.". J. Cell Biol. 149 (4): 761–6. doi:10.1083/jcb.149.4.761. PMC 2174556. PMID 10811817.
• Morrison EE, Wardleworth BN, Askham JM, Markham AF, Meredith DM (1999). "EB1, a protein which interacts with the APC tumour suppressor, is associated with the microtubule cytoskeleton throughout the cell cycle.". Oncogene 17 (26): 3471–7. doi:10.1038/sj.onc.1202247. PMID 10030671.
• Juwana JP, Henderikx P, Mischo A, Wadle A, Fadle N, Gerlach K, Arends JW, Hoogenboom H, Pfreundschuh M, Renner C (1999). "EB/RP gene family encodes tubulin binding proteins.". Int. J. Cancer 81 (2): 275–84. doi:10.1002/(SICI)1097-0215(19990412)81:2<275::AID-IJC18>3.0.CO;2-Z. PMID 10188731.
• Berrueta L, Tirnauer JS, Schuyler SC, Pellman D, Bierer BE (1999). "The APC-associated protein EB1 associates with components of the dynactin complex and cytoplasmic dynein intermediate chain.". Curr. Biol. 9 (8): 425–8. doi:10.1016/S0960-9822(99)80190-0. PMID 10226031.
• Nakagawa H, Koyama K, Murata Y, Morito M, Akiyama T, Nakamura Y (2000). "EB3, a novel member of the EB1 family preferentially expressed in the central nervous system, binds to a CNS-specific APC homologue.". Oncogene 19 (2): 210–6. doi:10.1038/sj.onc.1203308. PMID 10644998.
• Askham JM, Moncur P, Markham AF, Morrison EE (2000). "Regulation and function of the interaction between the APC tumour suppressor protein and EB1.". Oncogene 19 (15): 1950–8. doi:10.1038/sj.onc.1203498. PMID 10773885.
• Mimori-Kiyosue Y, Shiina N, Tsukita S (2000). "The dynamic behavior of the APC-binding protein EB1 on the distal ends of microtubules.". Curr. Biol. 10 (14): 865–8. doi:10.1016/S0960-9822(00)00600-X. PMID 10899006.
• Zhou XZ, Lu KP (2001). "The Pin2/TRF1-interacting protein PinX1 is a potent telomerase inhibitor.". Cell 107 (3): 347–59. doi:10.1016/S0092-8674(01)00538-4. PMID 11701125.
• Nakamura M, Zhou XZ, Kishi S, Lu KP (2002). "Involvement of the telomeric protein Pin2/TRF1 in the regulation of the mitotic spindle.". FEBS Lett. 514 (2-3): 193–8. doi:10.1016/S0014-5793(02)02363-3. PMID 11943150.
• Askham JM, Vaughan KT, Goodson HV, Morrison EE (2003). "Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome.". Mol. Biol. Cell 13 (10): 3627–45. doi:10.1091/mbc.E02-01-0061. PMC 129971. PMID 12388762.
• Tirnauer JS, Canman JC, Salmon ED, Mitchison TJ (2003). "EB1 targets to kinetochores with attached, polymerizing microtubules.". Mol. Biol. Cell 13 (12): 4308–16. doi:10.1091/mbc.E02-04-0236. PMC 138635. PMID 12475954.
• Ligon LA, Shelly SS, Tokito M, Holzbaur EL (2003). "The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization.". Mol. Biol. Cell 14 (4): 1405–17. doi:10.1091/mbc.E02-03-0155. PMC 153110. PMID 12686597.
• Hayashi I, Ikura M (2003). "Crystal structure of the amino-terminal microtubule-binding domain of end-binding protein 1 (EB1).". J. Biol. Chem. 278 (38): 36430–4. doi:10.1074/jbc.M305773200. PMID 12857735.
• Bu W, Su LK (2004). "Characterization of functional domains of human EB1 family proteins.". J. Biol. Chem. 278 (50): 49721–31. doi:10.1074/jbc.M306194200. PMID 14514668.
• Bieling P, Laan L, Schek H, Munteanu EL, Sandblad L, Dogterom M, Brunner D, Surrey T (2007). "Reconstitution of a microtubule plus-end tracking system in vitro.". Nature. 13 (7172): 1100–5. doi:10.1038/nature06386. PMID 18059460.
• Maurer SP, Fourniol FJ, Bohner G, Moores CA, Surrey T (2012). "EBs recognize a nucleotide-dependent structural cap at growing microtubule ends.". Cell. 149 (2): 371–82. doi:10.1016/j.cell.2012.02.049. PMID 22500803.
• Nakamura S, Grigoriev I, Nogi T, Hamaji T, Cassimeris L, Mimori-Kiyosue Y (2012). "Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells.". PLOS ONE 7 (12): e51442. doi:10.1371/journal.pone.0051442. PMID 23251535.
• Zanic M, Widlund PO, Hyman AA, Howard J (2013). "Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels.". Nat Cell Biol. 15 (6): 688–93. doi:10.1038/ncb2744. PMID 23666085.
• Jie, Chen (2014). "Phosphoregulation of the dimerization and functions of end-binding protein 1.". [J]. Protein & Cell. 5 (10): 795–799.