MCM10

Minichromosome maintenance complex component 10
Identifiers
Symbols	MCM10 ; CNA43; DNA43; PRO2249
External IDs	OMIM: 609357 MGI: 1917274 HomoloGene: 41275 GeneCards: MCM10 Gene
Gene ontology Molecular function • DNA replication origin binding • double-stranded DNA binding • single-stranded DNA binding • protein binding • identical protein binding • metal ion binding Cellular component • nucleus • nucleoplasm • nucleolus • cytoplasm • replication fork protection complex Biological process • G1/S transition of mitotic cell cycle • mitotic cell cycle • DNA replication • DNA replication initiation • cell proliferation Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	55388	70024
Ensembl	ENSG00000065328	ENSMUSG00000026669
UniProt	Q7L590	Q0VBD2
RefSeq (mRNA)	NM_018518	NM_001305259
RefSeq (protein)	NP_060988	NP_001292188
Location (UCSC)	Chr 10: 13.16 – 13.21 Mb	Chr 2: 4.99 – 5.01 Mb
PubMed search

Protein MCM10 homolog is a protein that in humans is encoded by the MCM10 gene.^[1][2][3]

Function

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified.^[3]

Interactions

MCM10 has been shown to interact with ORC2L.^[1]

References

1. Izumi M, Yanagi K, Mizuno T, Yokoi M, Kawasaki Y, Moon KY, Hurwitz J, Yatagai F, Hanaoka F (Dec 2000). "The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase". Nucleic Acids Res. 28 (23): 4769–77. doi:10.1093/nar/28.23.4769. PMC 115166. PMID 11095689.
2. Chattopadhyay S, Bielinsky AK (Sep 2007). "Human Mcm10 regulates the catalytic subunit of DNA polymerase-alpha and prevents DNA damage during replication". Mol. Biol. Cell 18 (10): 4085–95. doi:10.1091/mbc.E06-12-1148. PMC 1995709. PMID 17699597.
3. "Entrez Gene: MCM10 MCM10 minichromosome maintenance deficient 10 (S. cerevisiae)".

Further reading

• Morimoto S (2002). "[Abnormal myocardial contractile regulation mechanism in familial hypertrophic and dilated cardiomyopathies--functional analysis of molecular mutant troponin in in-vitro]". Fukuoka Igaku Zasshi 93 (1): 1–5. PMID 11889827.
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
• Izumi M, Yatagai F, Hanaoka F (2001). "Cell cycle-dependent proteolysis and phosphorylation of human Mcm10". J. Biol. Chem. 276 (51): 48526–31. doi:10.1074/jbc.M107190200. PMID 11602595.
• Wohlschlegel JA, Dhar SK, Prokhorova TA, Dutta A, Walter JC (2002). "Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and stimulates origin binding of Cdc45". Mol. Cell 9 (2): 233–40. doi:10.1016/S1097-2765(02)00456-2. PMID 11864598.
• Christensen TW, Tye BK (2003). "Drosophila MCM10 interacts with members of the prereplication complex and is required for proper chromosome condensation". Mol. Biol. Cell 14 (6): 2206–15. doi:10.1091/mbc.E02-11-0706. PMC 194871. PMID 12808023.
• Cook CR, Kung G, Peterson FC, Volkman BF, Lei M (2003). "A novel zinc finger is required for Mcm10 homocomplex assembly". J. Biol. Chem. 278 (38): 36051–8. doi:10.1074/jbc.M306049200. PMID 12844493.
• Izumi M, Yatagai F, Hanaoka F (2004). "Localization of human Mcm10 is spatially and temporally regulated during the S phase". J. Biol. Chem. 279 (31): 32569–77. doi:10.1074/jbc.M314017200. PMID 15136575.
• Yoshida K, Inoue I (2004). "Expression of MCM10 and TopBP1 is regulated by cell proliferation and UV irradiation via the E2F transcription factor". Oncogene 23 (37): 6250–60. doi:10.1038/sj.onc.1207829. PMID 15195143.
• Ramachandran N, Hainsworth E, Bhullar B, Eisenstein S, Rosen B, Lau AY, Walter JC, LaBaer J (2004). "Self-assembling protein microarrays". Science 305 (5680): 86–90. doi:10.1126/science.1097639. PMID 15232106.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Zhu W, Ukomadu C, Jha S, Senga T, Dhar SK, Wohlschlegel JA, Nutt LK, Kornbluth S, Dutta A (2007). "Mcm10 and And-1/CTF4 recruit DNA polymerase alpha to chromatin for initiation of DNA replication". Genes Dev. 21 (18): 2288–99. doi:10.1101/gad.1585607. PMC 1973143. PMID 17761813.
• Robertson PD, Warren EM, Zhang H, Friedman DB, Lary JW, Cole JL, Tutter AV, Walter JC, Fanning E, Eichman BF (2008). "Domain architecture and biochemical characterization of vertebrate Mcm10". J. Biol. Chem. 283 (6): 3338–48. doi:10.1074/jbc.M706267200. PMC 2753450. PMID 18065420.
• Warren EM, Vaithiyalingam S, Haworth J, Greer B, Bielinsky AK, Chazin WJ, Eichman BF (2008). "Structural basis for DNA binding by replication initiator Mcm10". Structure 16 (12): 1892–901. doi:10.1016/j.str.2008.10.005. PMC 2636851. PMID 19081065.