MCM8

Minichromosome maintenance complex component 8
Identifiers
Symbols	MCM8 ; C20orf154; POF10; dJ967N21.5
External IDs	OMIM: 608187 MGI: 1913884 HomoloGene: 12001 GeneCards: MCM8 Gene
EC number	3.6.4.12
Gene ontology Molecular function • DNA binding • helicase activity • protein binding • ATP binding Cellular component • nucleus • nucleoplasm • MCM8-MCM9 complex Biological process • G1/S transition of mitotic cell cycle • mitotic cell cycle • double-strand break repair via homologous recombination • DNA replication • DNA strand elongation involved in DNA replication • cellular response to DNA damage stimulus • female gamete generation • male gamete generation Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	84515	66634
Ensembl	ENSG00000125885	ENSMUSG00000027353
UniProt	Q9UJA3	Q9CWV1
RefSeq (mRNA)	NM_001281520	NM_001291054
RefSeq (protein)	NP_001268449	NP_001277983
Location (UCSC)	Chr 20: 5.95 – 6 Mb	Chr 2: 132.82 – 132.84 Mb
PubMed search

DNA replication licensing factor MCM8 is a protein that in humans is encoded by the MCM8 gene.^[1][2]

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the MCM proteins. This protein has been shown to co-immunoprecipitate with MCM4, 6 and 7, which suggests that it may interact with other MCM proteins and play a role in DNA replication. Alternatively spliced transcript variants encoding distinct isoforms have been described.^[2]

See also

• Mini Chromosome Maintenance

References

1. Gozuacik D, Chami M, Lagorce D, Faivre J, Murakami Y, Poch O, Biermann E, Knippers R, Brechot C, Paterlini-Brechot P (Jan 2003). "Identification and functional characterization of a new member of the human Mcm protein family: hMcm8". Nucleic Acids Res 31 (2): 570–9. doi:10.1093/nar/gkg136. PMC 140502. PMID 12527764.
2. "Entrez Gene: MCM8 MCM8 minichromosome maintenance deficient 8 (S. cerevisiae)".

Further reading

• Volkening M, Hoffmann I (2005). "Involvement of human MCM8 in prereplication complex assembly by recruiting hcdc6 to chromatin". Mol. Cell. Biol. 25 (4): 1560–8. doi:10.1128/MCB.25.4.1560-1568.2005. PMC 548026. PMID 15684404.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Johnson EM, Kinoshita Y, Daniel DC (2003). "A new member of the MCM protein family encoded by the human MCM8 gene, located contrapodal to GCD10 at chromosome band 20p12.3-13". Nucleic Acids Res. 31 (11): 2915–25. doi:10.1093/nar/gkg395. PMC 156728. PMID 12771218.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20". Nature 414 (6866): 865–71. doi:10.1038/414865a. PMID 11780052.
• Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548.