MED21

Mediator complex subunit 21
Identifiers
Symbols	MED21 ; SRB7; SURB7; hSrb7
External IDs	OMIM: 603800 MGI: 1347064 HomoloGene: 3148 GeneCards: MED21 Gene
Gene ontology Molecular function • RNA polymerase II transcription cofactor activity • transcription coactivator activity • DNA-directed RNA polymerase activity • protein binding • ubiquitin protein ligase activity Cellular component • ubiquitin ligase complex • mediator complex Biological process • blastocyst development • transcription, DNA-templated • regulation of transcription from RNA polymerase II promoter • protein ubiquitination • stem cell maintenance • positive regulation of transcription from RNA polymerase II promoter Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	9412	108098
Ensembl	ENSG00000152944	ENSMUSG00000030291
UniProt	Q13503	Q9CQ39
RefSeq (mRNA)	NM_001271811	NM_025315
RefSeq (protein)	NP_001258740	NP_079591
Location (UCSC)	Chr 12: 27.02 – 27.07 Mb	Chr 6: 146.64 – 146.65 Mb
PubMed search

Mediator of RNA polymerase II transcription subunit 21 is an enzyme that in humans is encoded by the MED21 gene.^[1][2]

Interactions

MED21 has been shown to interact with:

• BRCA1,^[3]
• CDK8,^[4][5][6][7]
• GTF2F1,^[5][3]
• GTF2H4,^[3]
• MED6,^[8] and
• POLR2A.^[4][5][3]

References

1. Chao DM, Gadbois EL, Murray PJ, Anderson SF, Sonu MS, Parvin JD, Young RA (Apr 1996). "A mammalian SRB protein associated with an RNA polymerase II holoenzyme". Nature 380 (6569): 82–5. doi:10.1038/380082a0. PMID 8598913.
2. "Entrez Gene: SURB7 SRB7 suppressor of RNA polymerase B homolog (yeast)".
3. Scully R, Anderson SF, Chao DM, Wei W, Ye L, Young RA, Livingston DM, Parvin JD (May 1997). "BRCA1 is a component of the RNA polymerase II holoenzyme". Proc. Natl. Acad. Sci. U.S.A. 94 (11): 5605–10. doi:10.1073/pnas.94.11.5605. PMC 20825. PMID 9159119.
4. Cho H, Orphanides G, Sun X, Yang XJ, Ogryzko V, Lees E, Nakatani Y, Reinberg D (Sep 1998). "A human RNA polymerase II complex containing factors that modify chromatin structure". Mol. Cell. Biol. 18 (9): 5355–63. PMC 109120. PMID 9710619.
5. Suñé C, Hayashi T, Liu Y, Lane WS, Young RA, Garcia-Blanco MA (Oct 1997). "CA150, a nuclear protein associated with the RNA polymerase II holoenzyme, is involved in Tat-activated human immunodeficiency virus type 1 transcription". Mol. Cell. Biol. 17 (10): 6029–39. PMC 232452. PMID 9315662.
6. Ito M, Yuan CX, Malik S, Gu W, Fondell JD, Yamamura S, Fu ZY, Zhang X, Qin J, Roeder RG (Mar 1999). "Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators". Mol. Cell 3 (3): 361–70. doi:10.1016/s1097-2765(00)80463-3. PMID 10198638.
7. Kang YK, Guermah M, Yuan CX, Roeder RG (Mar 2002). "The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro". Proc. Natl. Acad. Sci. U.S.A. 99 (5): 2642–7. doi:10.1073/pnas.261715899. PMC 122401. PMID 11867769.
8. Sun X, Zhang Y, Cho H, Rickert P, Lees E, Lane W, Reinberg D (Aug 1998). "NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription". Mol. Cell 2 (2): 213–22. doi:10.1016/s1097-2765(00)80131-8. PMID 9734358.

Further reading

• Zheng XM, Black D, Chambon P, Egly JM (1990). "Sequencing and expression of complementary DNA for the general transcription factor BTF3.". Nature 344 (6266): 556–9. doi:10.1038/344556a0. PMID 2320128.
• Maldonado E, Shiekhattar R, Sheldon M, Cho H, Drapkin R, Rickert P, Lees E, Anderson CW, Linn S, Reinberg D (1996). "A human RNA polymerase II complex associated with SRB and DNA-repair proteins.". Nature 381 (6577): 86–9. doi:10.1038/381086a0. PMID 8609996.
• Cujec TP, Cho H, Maldonado E, Meyer J, Reinberg D, Peterlin BM (1997). "The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme.". Mol. Cell. Biol. 17 (4): 1817–23. PMC 232028. PMID 9121429.
• Scully R, Anderson SF, Chao DM, Wei W, Ye L, Young RA, Livingston DM, Parvin JD (1997). "BRCA1 is a component of the RNA polymerase II holoenzyme.". Proc. Natl. Acad. Sci. U.S.A. 94 (11): 5605–10. doi:10.1073/pnas.94.11.5605. PMC 20825. PMID 9159119.
• Pan G, Aso T, Greenblatt J (1997). "Interaction of elongation factors TFIIS and elongin A with a human RNA polymerase II holoenzyme capable of promoter-specific initiation and responsive to transcriptional activators.". J. Biol. Chem. 272 (39): 24563–71. doi:10.1074/jbc.272.39.24563. PMID 9305922.
• Cho H, Orphanides G, Sun X, Yang XJ, Ogryzko V, Lees E, Nakatani Y, Reinberg D (1998). "A human RNA polymerase II complex containing factors that modify chromatin structure.". Mol. Cell. Biol. 18 (9): 5355–63. PMC 109120. PMID 9710619.
• Sun X, Zhang Y, Cho H, Rickert P, Lees E, Lane W, Reinberg D (1998). "NAT, a human complex containing Srb polypeptides that functions as a negative regulator of activated transcription.". Mol. Cell 2 (2): 213–22. doi:10.1016/S1097-2765(00)80131-8. PMID 9734358.
• Garriga J, Peng J, Parreño M, Price DH, Henderson EE, Graña X (1999). "Upregulation of cyclin T1/CDK9 complexes during T cell activation.". Oncogene 17 (24): 3093–102. doi:10.1038/sj.onc.1202548. PMID 9872325.
• Gu W, Malik S, Ito M, Yuan CX, Fondell JD, Zhang X, Martinez E, Qin J, Roeder RG (1999). "A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation.". Mol. Cell 3 (1): 97–108. doi:10.1016/S1097-2765(00)80178-1. PMID 10024883.
• Ramanathan Y, Rajpara SM, Reza SM, Lees E, Shuman S, Mathews MB, Pe'ery T (2001). "Three RNA polymerase II carboxyl-terminal domain kinases display distinct substrate preferences.". J. Biol. Chem. 276 (14): 10913–20. doi:10.1074/jbc.M010975200. PMID 11278802.
• Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y (2001). "Protein-protein interaction panel using mouse full-length cDNAs.". Genome Res. 11 (10): 1758–65. doi:10.1101/gr.180101. PMC 311163. PMID 11591653.
• Ge K, Guermah M, Yuan CX, Ito M, Wallberg AE, Spiegelman BM, Roeder RG (2002). "Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated adipogenesis.". Nature 417 (6888): 563–7. doi:10.1038/417563a. PMID 12037571.
• Sato S, Tomomori-Sato C, Banks CA, Parmely TJ, Sorokina I, Brower CS, Conaway RC, Conaway JW (2004). "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex.". J. Biol. Chem. 278 (50): 49671–4. doi:10.1074/jbc.C300444200. PMID 14576168.
• Wallberg AE, Yamamura S, Malik S, Spiegelman BM, Roeder RG (2004). "Coordination of p300-mediated chromatin remodeling and TRAP/mediator function through coactivator PGC-1alpha.". Mol. Cell 12 (5): 1137–49. doi:10.1016/S1097-2765(03)00391-5. PMID 14636573.
• Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW (2004). "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2.". J. Biol. Chem. 279 (7): 5846–51. doi:10.1074/jbc.M312523200. PMID 14638676.
• Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.". Mol. Cell 14 (5): 685–91. doi:10.1016/j.molcel.2004.05.006. PMID 15175163.
• Nevado J, Tenbaum SP, Aranda A (2005). "hSrb7, an essential human Mediator component, acts as a coactivator for the thyroid hormone receptor.". Mol. Cell. Endocrinol. 222 (1-2): 41–51. doi:10.1016/j.mce.2004.05.003. PMID 15249124.