MRPS5

Mitochondrial ribosomal protein S5
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 3J9M​
Identifiers
Symbols	MRPS5 ; MRP-S5; S5mt
External IDs	OMIM: 611972 MGI: 1924971 HomoloGene: 32726 GeneCards: MRPS5 Gene
Gene ontology Molecular function • structural constituent of ribosome • poly(A) RNA binding Cellular component • mitochondrion • mitochondrial inner membrane • mitochondrial small ribosomal subunit Biological process • organelle organization • mitochondrial translation • mitochondrial translational initiation • mitochondrial translational elongation • mitochondrial translational termination Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	64969	77721
Ensembl	ENSG00000144029	ENSMUSG00000027374
UniProt	P82675	Q99N87
RefSeq (mRNA)	NM_031902	NM_029963
RefSeq (protein)	NP_114108	NP_084239
Location (UCSC)	Chr 2: 95.09 – 95.15 Mb	Chr 2: 127.59 – 127.61 Mb
PubMed search

28S ribosomal protein S5, mitochondrial is a protein that in humans is encoded by the MRPS5 gene.^[1]

Function

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S5P family. Pseudogenes corresponding to this gene are found on chromosomes 4q, 5q, and 18q.^[1]

Model organisms

Model organisms have been used in the study of MRPS5 function. A conditional knockout mouse line called Mrps5^{tm1b(EUCOMM)Wtsi} was generated at the Wellcome Trust Sanger Institute.^[2] Male and female animals underwent a standardized phenotypic screen^[3] to determine the effects of deletion.^[4][5][6][7] Additional screens performed: - In-depth immunological phenotyping^[8]

Mrps5 knockout mouse phenotype

Characteristic	Phenotype
All data available at.[3][8]
Peripheral blood leukocytes 6 Weeks	Abnormal
Haematology 6 Weeks	Abnormal
Insulin	Normal
Homozygous viability at P14	Abnormal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Heart weight	Normal
Salmonella infection	Normal
Cytotoxic T Cell Function	Normal
Spleen Immunophenotyping	Normal
Mesenteric Lymph Node Immunophenotyping	Normal
Bone Marrow Immunophenotyping	Normal
Influenza Challenge	Normal

References

1. "Entrez Gene: MRPS5 mitochondrial ribosomal protein S5".
2. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
3. "International Mouse Phenotyping Consortium".
4. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
5. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
6. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
7. White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
8. "Infection and Immunity Immunophenotyping (3i) Consortium".

Further reading

• Zhang Z, Gerstein M (May 2003). "Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome". Genomics 81 (5): 468–80. doi:10.1016/S0888-7543(03)00004-1. PMID 12706105.
• Kenmochi N, Suzuki T, Uechi T, Magoori M, Kuniba M, Higa S, Watanabe K, Tanaka T (Sep 2001). "The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders". Genomics 77 (1-2): 65–70. doi:10.1006/geno.2001.6622. PMID 11543634.
• Suzuki T, Terasaki M, Takemoto-Hori C, Hanada T, Ueda T, Wada A, Watanabe K (Aug 2001). "Proteomic analysis of the mammalian mitochondrial ribosome. Identification of protein components in the 28 S small subunit". The Journal of Biological Chemistry 276 (35): 33181–95. doi:10.1074/jbc.M103236200. PMID 11402041.
• Koc EC, Burkhart W, Blackburn K, Koc H, Moseley A, Spremulli LL (Mar 2001). "Identification of four proteins from the small subunit of the mammalian mitochondrial ribosome using a proteomics approach". Protein Science 10 (3): 471–81. doi:10.1110/ps.35301. PMC 2374141. PMID 11344316.
• Cavdar Koc E, Burkhart W, Blackburn K, Moseley A, Spremulli LL (Jun 2001). "The small subunit of the mammalian mitochondrial ribosome. Identification of the full complement of ribosomal proteins present". The Journal of Biological Chemistry 276 (22): 19363–74. doi:10.1074/jbc.M100727200. PMID 11279123.