MYO10

Myosin X
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2LW9​, 3AU4​, 3AU5​, 3PZD​
Identifiers
Symbols	MYO10 ; FLJ10639; FLJ21066; FLJ22268; FLJ43256; KIAA0799; MGC131988
External IDs	OMIM: 601481 MGI: 107716 HomoloGene: 36328 GeneCards: MYO10 Gene
Gene ontology Molecular function • protein binding • calmodulin binding • ATP binding • phosphatidylinositol-3,4,5-trisphosphate binding • spectrin binding • actin-dependent ATPase activity • actin filament binding • plus-end directed microfilament motor activity Cellular component • ruffle • nucleolus • cytoplasm • cytosol • plasma membrane • cell cortex • myosin complex • lamellipodium • filopodium membrane • filopodium tip • neuron projection • neuronal cell body Biological process • axon guidance • metabolic process • regulation of cell shape • positive regulation of cell-cell adhesion • cytoskeleton-dependent intracellular transport • Fc-gamma receptor signaling pathway involved in phagocytosis • innate immune response • regulation of filopodium assembly Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	4651	17909
Ensembl	ENSG00000145555	ENSMUSG00000022272
UniProt	Q9HD67	F8VQB6
RefSeq (mRNA)	NM_012334	NM_019472
RefSeq (protein)	NP_036466	NP_062345
Location (UCSC)	Chr 5: 16.66 – 16.94 Mb	Chr 15: 25.62 – 25.81 Mb
PubMed search

Myosin X, also known as MYO10, is a protein that in humans is encoded by the MYO10 gene.^[1][2][3]

Myosin X is an actin-based motor protein known to associate at the tips of filopodia.^[4]

Model organisms

Model organisms have been used in the study of MYO10 function. A conditional knockout mouse line called Myo10^{tm2(KOMP)Wtsi} was generated at the Wellcome Trust Sanger Institute.^[5] Male and female animals underwent a standardized phenotypic screen^[6] to determine the effects of deletion.^{[7][8][9][10]} Additional screens performed: - In-depth immunological phenotyping^[11]

Myo10 knockout mouse phenotype

Characteristic	Phenotype
All data available at.[6][11]
Peripheral blood leukocytes 6 Weeks	Abnormal
Haematology 6 Weeks	Normal
Insulin	Normal
Homozygous viability at P14	Normal
Homozygous Fertility	Normal
General Observations	Abnormal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Abnormal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Eye morphology	Abnormal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Heart weight	Normal
Salmonella infection	Normal
Cytotoxic T Cell Function	Normal
Spleen Immunophenotyping	Normal
Mesenteric Lymph Node Immunophenotyping	Normal
Epidermal Immune Composition	Normal
Influenza Challenge	Normal

References

1. "Entrez Gene: MYO10 myosin X".
2. Bement WM, Hasson T, Wirth JA, Cheney RE, Mooseker MS (Jul 1994). "Identification and overlapping expression of multiple unconventional myosin genes in vertebrate cell types". Proceedings of the National Academy of Sciences of the United States of America 91 (14): 6549–53. doi:10.1073/pnas.91.14.6549. PMC 44240. PMID 8022818.
3. Hasson T, Skowron JF, Gilbert DJ, Avraham KB, Perry WL, Bement WM, Anderson BL, Sherr EH, Chen ZY, Greene LA, Ward DC, Corey DP, Mooseker MS, Copeland NG, Jenkins NA (Sep 1996). "Mapping of unconventional myosins in mouse and human". Genomics 36 (3): 431–9. doi:10.1006/geno.1996.0488. PMID 8884266.
4. Berg JS, Cheney RE (Mar 2002). "Myosin-X is an unconventional myosin that undergoes intrafilopodial motility". Nature Cell Biology 4 (3): 246–50. doi:10.1038/ncb762. PMID 11854753.
5. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
6. "International Mouse Phenotyping Consortium".
7. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
8. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
9. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
10. White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
11. "Infection and Immunity Immunophenotyping (3i) Consortium".

Further reading

• Hasson T, Skowron JF, Gilbert DJ, Avraham KB, Perry WL, Bement WM, Anderson BL, Sherr EH, Chen ZY, Greene LA, Ward DC, Corey DP, Mooseker MS, Copeland NG, Jenkins NA (Sep 1996). "Mapping of unconventional myosins in mouse and human". Genomics 36 (3): 431–9. doi:10.1006/geno.1996.0488. PMID 8884266.
• Nagase T, Ishikawa K, Suyama M, Kikuno R, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Oct 1998). "Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research 5 (5): 277–86. doi:10.1093/dnares/5.5.277. PMID 9872452.
• Rojas K, Serrano de la Peña L, Gallardo T, Simmons A, Nyce K, McGrath R, Considine E, Vasko AJ, Peterson E, Grady D, Cox R, Andrew LJ, Lovett M, Overhauser J, Williams CJ (Dec 1999). "Physical map and characterization of transcripts in the candidate interval for familial chondrocalcinosis at chromosome 5p15.1". Genomics 62 (2): 177–83. doi:10.1006/geno.1999.5997. PMID 10610710.
• Berg JS, Derfler BH, Pennisi CM, Corey DP, Cheney RE (Oct 2000). "Myosin-X, a novel myosin with pleckstrin homology domains, associates with regions of dynamic actin". Journal of Cell Science. 113. 113 Pt 19: 3439–51. PMID 10984435.
• Rogers MS, Strehler EE (Apr 2001). "The tumor-sensitive calmodulin-like protein is a specific light chain of human unconventional myosin X". The Journal of Biological Chemistry 276 (15): 12182–9. doi:10.1074/jbc.M010056200. PMID 11278607.
• Cox D, Berg JS, Cammer M, Chinegwundoh JO, Dale BM, Cheney RE, Greenberg S (Jul 2002). "Myosin X is a downstream effector of PI(3)K during phagocytosis". Nature Cell Biology 4 (7): 469–77. doi:10.1038/ncb805. PMID 12055636.
• Navarro-Lérida I, Martínez Moreno M, Roncal F, Gavilanes F, Albar JP, Rodríguez-Crespo I (Feb 2004). "Proteomic identification of brain proteins that interact with dynein light chain LC8". Proteomics 4 (2): 339–46. doi:10.1002/pmic.200300528. PMID 14760703.
• Zhang H, Berg JS, Li Z, Wang Y, Lång P, Sousa AD, Bhaskar A, Cheney RE, Strömblad S (Jun 2004). "Myosin-X provides a motor-based link between integrins and the cytoskeleton". Nature Cell Biology 6 (6): 523–31. doi:10.1038/ncb1136. PMID 15156152.
• Tokuo H, Ikebe M (Jun 2004). "Myosin X transports Mena/VASP to the tip of filopodia". Biochemical and Biophysical Research Communications 319 (1): 214–20. doi:10.1016/j.bbrc.2004.04.167. PMID 15158464.
• Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S (Jan 2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Research 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
• Sousa AD, Berg JS, Robertson BW, Meeker RB, Cheney RE (Jan 2006). "Myo10 in brain: developmental regulation, identification of a headless isoform and dynamics in neurons". Journal of Cell Science 119 (Pt 1): 184–94. doi:10.1242/jcs.02726. PMID 16371656.
• Bohil AB, Robertson BW, Cheney RE (Aug 2006). "Myosin-X is a molecular motor that functions in filopodia formation". Proceedings of the National Academy of Sciences of the United States of America 103 (33): 12411–6. doi:10.1073/pnas.0602443103. PMC 1567893. PMID 16894163.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (Nov 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.