Mannan-binding lectin-associated serine protease-2

Mannan-binding lectin-associated serine protease-2
Identifiers
EC number	3.4.21.104
CAS number	214915-16-9
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Mannan-binding lectin-associated serine protease-2 (EC 3.4.21.104, MASP-2, MASP2, MBP-associated serine protease-2, mannose-binding lectin-associated serine protease-2, p100, mannan-binding lectin-associated serine peptidase 2) is an enzyme.^{[1][2][3][4][5][6][7]} This enzyme catalyses the following chemical reaction

Selective cleavage after Arg²²³ in complement component C2 (-Ser-Leu-Gly-Arg-Lys-Ile-Gln-Ile) and after Arg⁷⁶ in complement component C4 (-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Ile)

This mannan-binding lectin (MBL) recognizes patterns of neutral carbohydrates, such as mannose and N-acetylglucosamine.

References

1. Matsushita, M. and Fujita, T. (1992). "Activation of the classical complement pathway by mannose-binding protein in association with a novel C1s-like serine protease". J. Exp. Med. 176 (6): 1497–1502. doi:10.1084/jem.176.6.1497. PMID 1460414.
2. Thiel, S., Vorup-Jensen, T., Stover, C.M., Schwaeble, W., Laursen, S.B., Poulsen, K., Willis, A.C., Eggleton, P., Hansen, S., Holmskov, U., Reid, K.B. and Jensenius, J.C. (1997). "A second serine protease associated with mannan-binding lectin that activates complement". Nature 386 (6624): 506–510. doi:10.1038/386506a0. PMID 9087411.
3. Rossi, V., Cseh, S., Bally, I., Thielens, N.M., Jensenius, J.C. and Arlaud, G.J. (2001). "Substrate specificities of recombinant mannan-binding lectin-associated serine proteases-1 and -2". J. Biol. Chem. 276 (44): 40880–40887. doi:10.1074/jbc.M105934200. PMID 11527969.
4. Ambrus, G., Gal, P., Kojima, M., Szilagyi, K., Balczer, J., Antal, J., Graf, L., Laich, A., Moffatt, B.E., Schwaeble, W., Sim, R.B. and Zavodszky, P. (2003). "Natural substrates and inhibitors of mannan-binding lectin-associated serine protease-1 and -2: a study on recombinant catalytic fragments". J. Immunol. 170 (3): 1374–1382. doi:10.4049/jimmunol.170.3.1374. PMID 12538697.
5. Harmat, V., Gal, P., Kardos, J., Szilagyi, K., Ambrus, G., Vegh, B., Naray-Szabo, G. and Zavodszky, P. (2004). "The structure of MBL-associated serine protease-2 reveals that identical substrate specificities of C1s and MASP-2 are realized through different sets of enzyme-substrate interactions". J. Mol. Biol. 342 (5): 1533–1546. doi:10.1016/j.jmb.2004.07.014. PMID 15364579.
6. Chen, C.B. and Wallis, R. (2004). "Two mechanisms for mannose-binding protein modulation of the activity of its associated serine proteases". J. Biol. Chem. 279 (25): 26058–26065. doi:10.1074/jbc.M401318200. PMID 15060079.
7. Teillet, F., Dublet, B., Andrieu, J.P., Gaboriaud, C., Arlaud, G.J. and Thielens, N.M. (2005). "The two major oligomeric forms of human mannan-binding lectin: chemical characterization, carbohydrate-binding properties, and interaction with MBL-associated serine proteases". J. Immunol. 174 (5): 2870–2877. doi:10.4049/jimmunol.174.5.2870. PMID 15728497.

External links

• Mannan-binding lectin-associated serine protease-2 at the US National Library of Medicine Medical Subject Headings (MeSH)