Middlebrook 7H10 Agar
Middlebrook 7H10 Agar is a solid growth medium specially used for culture of Mycobacterium, notably Mycobacterium tuberculosis.[1] It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.
Composition
- Ammonium Sulfate
- Monopotassium phosphate
- Disodium phosphate
- Sodium Citrate
- Magnesium Sulfate
- sodium
- Zinc Sulfate
- Copper Sulfate
- L-Glutamic Acid (sodium salt)
- Ferric Ammonium Citrate
- Pyridoxine Hydrochloride
- Biotin
- Malachite Green
- Agar
Cultures should be read within 5-7 days after inoculation and once a week thereafter for up to 8 weeks.
See also
References
External links
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