MinC

The MinC protein is one of three proteins encoded by the minB operon and which is required to generate pole to pole oscillations prior to bacterial cell division as a means of specifying the midzone of the cell. This function is achieved by preventing the formation of the divisome Z-ring around the poles.[1]

History

When initially discovered in E.coli, MinC was believed to form a stable cap by binding with MinD at the bacterial caps in a stable and static manner, thereby specifying the mid-zone of the cell by lower-concentration or lack of this complex in that region, alleviating inhibition of Z-ring formation. However, Raskin & de Boer applied live-cell imaging with GFP fusion proteins to reveal a dynamic interaction of the Min proteins, demonstrating that MinC and MinD, with the essential help of MinE,[2] actually rapidly oscillate between the two poles in a non-static manner.[3] MinC is found in diverse genera, including Neisseria, Bacillus and Synechocystis, but is absent in an equally wide assortment of genera (e.g. Caulobacter, Mycobacterium). Many bacteria that lack MinC carry alternative proteins that can position their Z-ring.[4]

Function

MinC is known to dimerize[5] and functions as an FtsZ protein inhibitor, antagonizing its structure assembly when in a high enough concentration. The protein has the ability to disassemble the ZapA, ZapB and ZipA proteins which preferentially bind to the FtsZ scaffold protein and which are needed to initiate cell division. Concentrations of MinC are lowest at the mid-zone of bacterial cells, thereby permitting septum formation and scaffold assembly at the cellular mid-zone since the Min System proteins (MinCDE) are lowest in concentration in this region, alleviating the physical inhibition on the proteins required to initiate division. [6]

References

  1. ↑ Pichoff S, Lutkenhaus J (2001). "Echerichia coli division inhibitor MinCD blocks separation by preventing Z-ring formation". Journal of Bacteriology 183 (22): 6630–6635. doi:10.1128/JB.183.22.6630-6635.2001. PMID 295494.
  2. ↑ de Boer PA, Crossley RE, Rothfield LI (1989). "A division inhibitor and a topological specificity factor coded for by the minicell locus determine proper placement of the division septum in E. coli". Cell 56 (4): 641–9. doi:10.1016/0092-8674(89)90586-2. PMID 2645057.
  3. ↑ Raskin DM, de Boer PA (1999). "Rapid pole-to-pole oscillation of a protein required for directing division to the middle of Escherichia coli". Proceedings of the National Academy of Sciences of the United States of America 96 (9): 4971–6. doi:10.1073/pnas.96.9.4971. PMC 21801. PMID 10220403.
  4. ↑ Rowlett VW, Margolin W (2015). "The Min system and other nucleoid-independent regulators of Z ring positioning". Frontiers in Microbiology 6: 478. doi:10.3389/fmicb.2015.00478. PMC 4429545. PMID 26029202.
  5. ↑ Suzanne C. Cordell, Rebecca E. Anderson, and Jan Löwe (2001). "Crystal structure of the bacterial cell division inhibitor MinC". The EMBO Journal 20 (10): 2454–2461. doi:10.1093/emboj/20.10.2454. PMID 125452.
  6. ↑ Hu Z, Mukherjee A, Pichoff S, Lutkenhaus J (1999). "The MinC Component of the Division Site Selection System in Escherichia Coli interacts with FtsZ to Prevent Polymerization". PNAS 96 (26): 14819–14824. doi:10.1073/pnas.96.26.14819. PMID 24731.
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