Nucleic acid methods
For protein methods, see Protein methods.
Nucleic acid methods are the techniques used to study nucleic acids (DNA and RNA).
Purification
- Phenol–chloroform extraction
- Minicolumn purification
- RNA extraction
- Boom method
- SCODA DNA purification
Quantification
- Abundance in weight: spectroscopic quantification
- Absolute abundance in number: quantitative PCR
- High-throughput relative abundance: DNA microarray
- High-throughput absolute abundance: SAGE
- Size: gel electrophoresis
Synthesis
- De novo: oligonucleotide synthesis
- Amplification: PCR
Kinetics
Gene function
Other
- Bisulfite sequencing
- DNA sequencing
- Expression cloning
- Fluorescence in situ hybridization
- Lab-on-a-chip
- Nucleic acid simulations
- Northern blot
- Nuclear run-on assay
- Radioactivity in biological research
- Southern blot
- Sucrose gradient centrifugation
- Toeprinting assay
- Several bioinformatics methods, such as RNA structure prediction
See also
References
- ↑ Tang, Wei; Hu, Shichao; Wang, Huaming; Zhao, Yan; Li, Na; Liu, Feng (23 September 2014). "A universal molecular translator for non-nucleic acid targets that enables dynamic DNA assemblies and logic operations". Chem. Commun. 50 (92): 14352–14355. doi:10.1039/C4CC07041K.
- ↑ Ihalainen, Petri; Pettersson, Fredrik; Pesonen, Markus; Viitala, Tapani; Määttänen, Anni; Österbacka, Ronald; Peltonen, Jouko (7 March 2014). "An impedimetric study of DNA hybridization on paper-supported inkjet-printed gold electrodes". Nanotechnology 25 (9): 094009. doi:10.1088/0957-4484/25/9/094009.
- ↑ Berney, H.; Oliver, K. (2005). "Dual polarization interferometry size and density characterisation of DNA immobilisation and hybridisation". Biosensors and Bioelectronics 21 (4): 618–626. doi:10.1016/j.bios.2004.12.024. PMID 16202875.
- ↑ Dixon, Matthew C. (July 2008). "Quartz Crystal Microbalance with Dissipation Monitoring: Enabling Real-Time Characterization of Biological Materials and Their Interactions". Journal of Biomolecular Techniques 19 (3): 151–158. PMC 2563918. PMID 19137101.
External links
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