Mycothiol-dependent formaldehyde dehydrogenase

mycothiol-dependent formaldehyde dehydrogenase
Identifiers
EC number	1.2.1.66
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a mycothiol-dependent formaldehyde dehydrogenase (EC 1.2.1.66) is an enzyme that catalyzes the chemical reaction

formaldehyde + mycothiol + NAD⁺ $\rightleftharpoons$ S-formylmycothiol + NADH + 2 H⁺

The 3 substrates of this enzyme are formaldehyde, mycothiol, and NAD⁺, whereas its 3 products are S-formylmycothiol, NADH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is formaldehyde:NAD+ oxidoreductase (mycothiol-formylating). This enzyme is also called NAD/factor-dependent formaldehyde dehydrogenase.

References

• Misset-Smits M, van Ophem PW, Sakuda S, Duine JA (1997). "Mycothiol, 1-O-(2'-[N-acetyl-L-cysteinyl]amido-2'-deoxy-alpha-D-glucopyranosyl) -D- myo-inositol, is the factor of NAD/factor-dependent formaldehyde dehydrogenase". FEBS Lett. 409 (2): 221–2. doi:10.1016/S0014-5793(97)00510-3. PMID 9202149.
• Norin A, Van Ophem PW, Piersma SR, Persson B, Duine JA, Jornvall H (1997). "Mycothiol-dependent formaldehyde dehydrogenase, a prokaryotic medium-chain dehydrogenase/reductase, phylogenetically links different eukaroytic alcohol dehydrogenases--primary structure, conformational modelling and functional correlations". Eur. J. Biochem. 248 (2): 282–9. doi:10.1111/j.1432-1033.1997.00282.x. PMID 9346279.