Myosin head

Myosin_head

scallop myosin in the near rigor conformation
Identifiers
Symbol Myosin_head
Pfam PF00063
Pfam clan CL0023
InterPro IPR001609
PROSITE PDOC00017
SCOP 1mys
SUPERFAMILY 1mys
CDD cd00124

Muscle contraction is caused by the sliding action of the thick filaments over the thin filaments of the myofibril. Myosin is a major component of thick filaments and exists as a hexamer of 2 heavy chains,[1] 2 alkali light chains, and 2 regulatory light chains. The heavy chain can be subdivided into the N-terminal globular head and the C-terminal coiled-coil rod-like tail, although some forms have a globular region in their C-terminal. There are many cell-specific isoforms of myosin heavy chains, coded for by a multi-gene family.[2] Myosin interacts with actin to convert chemical energy, in the form of ATP, to mechanical energy.[3] The 3-D structure of the head portion of myosin has been determined [4] and a model for actin-myosin complex has been constructed.[5]

The globular head is well conserved,[4][6][7] and is key to contraction. Muscle contraction results from an attachment–detachment cycle between the myosin heads extending from myosin filaments and the sites on actin filaments. The myosin head first attaches to actin together with the products of ATP hydrolysis, performs a power stroke associated with release of hydrolysis products, and detaches from actin upon binding with new ATP. The detached myosin head then hydrolyses ATP, and performs a recovery stroke to restore its initial position. The strokes have been suggested to result from rotation of the lever arm domain around the converter domain, while the catalytic domain remains rigid.[8]

References

  1. Hayashida M, Maita T, Matsuda G (July 1991). "The primary structure of skeletal muscle myosin heavy chain: I. Sequence of the amino-terminal 23 kDa fragment". J. Biochem. 110 (1): 54–9. PMID 1939027.
  2. Eller M, Stedman HH, Sylvester JE, Fertels SH, Wu QL, Raychowdhury MK, Rubinstein NA, Kelly AM, Sarkar S (October 1989). "Human embryonic myosin heavy chain cDNA. Interspecies sequence conservation of the myosin rod, chromosomal locus and isoform specific transcription of the gene". FEBS Lett. 256 (1-2): 21–8. doi:10.1016/0014-5793(89)81710-7. PMID 2806546.
  3. Warrick HM, De Lozanne A, Leinwand LA, Spudich JA (December 1986). "Conserved protein domains in a myosin heavy chain gene from Dictyostelium discoideum". Proc. Natl. Acad. Sci. U.S.A. 83 (24): 9433–7. doi:10.1073/pnas.83.24.9433. PMC 387152. PMID 3540939.
  4. 1 2 Rayment I, Rypniewski WR, Schmidt-Bäse K, Smith R, Tomchick DR, Benning MM, Winkelmann DA, Wesenberg G, Holden HM (July 1993). "Three-dimensional structure of myosin subfragment-1: a molecular motor". Science 261 (5117): 50–8. doi:10.1126/science.8316857. PMID 8316857.
  5. Rayment I, Holden HM, Whittaker M, Yohn CB, Lorenz M, Holmes KC, Milligan RA (July 1993). "Structure of the actin-myosin complex and its implications for muscle contraction". Science 261 (5117): 58–65. doi:10.1126/science.8316858. PMID 8316858.
  6. Molloy JE, Burns JE, Kendrick-Jones J, Tregear RT, White DC (November 1995). "Movement and force produced by a single myosin head". Nature 378 (6553): 209–12. doi:10.1038/378209a0. PMID 7477328.
  7. Lewalle A, Steffen W, Stevenson O, Ouyang Z, Sleep J (March 2008). "Single-molecule measurement of the stiffness of the rigor myosin head". Biophysical Journal 94 (6): 2160–9. doi:10.1529/biophysj.107.119396. PMC 2257899. PMID 18065470. Retrieved 2011-06-17.
  8. Minoda H, Okabe T, Inayoshi Y, Miyakawa T, Miyauchi Y, Tanokura M, Katayama E, Wakabayashi T, Akimoto T, Sugi H (February 2011). "Electron microscopic evidence for the myosin head lever arm mechanism in hydrated myosin filaments using the gas environmental chamber". Biochemical and Biophysical Research Communications 405 (4): 651–6. doi:10.1016/j.bbrc.2011.01.087. PMID 21281603.

This article incorporates text from the public domain Pfam and InterPro IPR001609

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