NEK9

NIMA-related kinase 9
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 3ZKE​, 3ZKF​
Identifiers
Symbols	NEK9 ; NERCC; NERCC1; Nek8
External IDs	OMIM: 609798 MGI: 2387995 HomoloGene: 13222 ChEMBL: 5257 GeneCards: NEK9 Gene
EC number	2.7.11.1
Gene ontology Molecular function • protein serine/threonine kinase activity • protein binding • ATP binding • protein kinase binding • metal ion binding Cellular component • nucleus • cytosol Biological process • mitotic cell cycle • protein phosphorylation • mitotic nuclear division • mitotic nuclear envelope disassembly • cell division Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	91754	217718
Ensembl	ENSG00000119638	ENSMUSG00000034290
UniProt	Q8TD19	Q8K1R7
RefSeq (mRNA)	NM_033116	NM_145138
RefSeq (protein)	NP_149107	NP_660120
Location (UCSC)	Chr 14: 75.08 – 75.13 Mb	Chr 12: 85.3 – 85.34 Mb
PubMed search

Serine/threonine-protein kinase Nek9 is an enzyme that in humans is encoded by the NEK9 gene.^[1][2]

Interactions

NEK9 has been shown to interact with:

• NEK6,^[3][4][5]
• RAN,^[5] and
• SSRP1.^[6]

Model organisms

Model organisms have been used in the study of NEK9 function. A conditional knockout mouse line called Nek9^{tm1a(EUCOMM)Wtsi} was generated at the Wellcome Trust Sanger Institute.^[7] Male and female animals underwent a standardized phenotypic screen^[8] to determine the effects of deletion.^{[9][10][11][12]} Additional screens performed: - In-depth immunological phenotyping^[13]

Nek9 knockout mouse phenotype

Characteristic	Phenotype
All data available at.[8][13]	Peripheral blood leukocytes 6 Weeks	Normal
Insulin	Normal
Haematology 6 Weeks	Normal
Homozygous viability at P14	Abnormal
Recessive lethal study	Abnormal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Heart weight	Normal
Salmonella infection	Normal
Cytotoxic T Cell Function	Normal
Spleen Immunophenotyping	Normal
Mesenteric Lymph Node Immunophenotyping	Normal
Bone Marrow Immunophenotyping	Normal
Epidermal Immune Composition	Normal

References

1. Holland PM, Milne A, Garka K, Johnson RS, Willis C, Sims JE, Rauch CT, Bird TA, Virca GD (May 2002). "Purification, cloning, and characterization of Nek8, a novel NIMA-related kinase, and its candidate substrate Bicd2". The Journal of Biological Chemistry 277 (18): 16229–40. doi:10.1074/jbc.M108662200. PMID 11864968.
2. "Entrez Gene: NEK9 NIMA (never in mitosis gene a)- related kinase 9".
3. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology 3: 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
4. Belham C, Roig J, Caldwell JA, Aoyama Y, Kemp BE, Comb M, Avruch J (Sep 2003). "A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases". The Journal of Biological Chemistry 278 (37): 34897–909. doi:10.1074/jbc.M303663200. PMID 12840024.
5. Roig J, Mikhailov A, Belham C, Avruch J (Jul 2002). "Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression". Genes & Development 16 (13): 1640–58. doi:10.1101/gad.972202. PMC 186374. PMID 12101123.
6. Tan BC, Lee SC (Mar 2004). "Nek9, a novel FACT-associated protein, modulates interphase progression". The Journal of Biological Chemistry 279 (10): 9321–30. doi:10.1074/jbc.M311477200. PMID 14660563.
7. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
8. "International Mouse Phenotyping Consortium".
9. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
10. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
11. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
12. White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
13. "Infection and Immunity Immunophenotyping (3i) Consortium".

Further reading

• Pelka P, Scimè A, Mandalfino C, Joch M, Abdulla P, Whyte P (Jul 2007). "Adenovirus E1A proteins direct subcellular redistribution of Nek9, a NimA-related kinase". Journal of Cellular Physiology 212 (1): 13–25. doi:10.1002/jcp.20983. PMID 17443675.
• Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
• Wissing J, Jänsch L, Nimtz M, Dieterich G, Hornberger R, Kéri G, Wehland J, Daub H (Mar 2007). "Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry". Molecular & Cellular Proteomics 6 (3): 537–47. doi:10.1074/mcp.T600062-MCP200. PMID 17192257.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (Nov 2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983.
• Bowers AJ, Boylan JF (Mar 2004). "Nek8, a NIMA family kinase member, is overexpressed in primary human breast tumors". Gene 328: 135–42. doi:10.1016/j.gene.2003.12.002. PMID 15019993.
• Tan BC, Lee SC (Mar 2004). "Nek9, a novel FACT-associated protein, modulates interphase progression". The Journal of Biological Chemistry 279 (10): 9321–30. doi:10.1074/jbc.M311477200. PMID 14660563.
• Belham C, Roig J, Caldwell JA, Aoyama Y, Kemp BE, Comb M, Avruch J (Sep 2003). "A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the Nek6 and Nek7 kinases". The Journal of Biological Chemistry 278 (37): 34897–909. doi:10.1074/jbc.M303663200. PMID 12840024.
• Roig J, Mikhailov A, Belham C, Avruch J (Jul 2002). "Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression". Genes & Development 16 (13): 1640–58. doi:10.1101/gad.972202. PMC 186374. PMID 12101123.
• Ohara O, Nagase T, Mitsui G, Kohga H, Kikuno R, Hiraoka S, Takahashi Y, Kitajima S, Saga Y, Koseki H (Apr 2002). "Characterization of size-fractionated cDNA libraries generated by the in vitro recombination-assisted method". DNA Research 9 (2): 47–57. doi:10.1093/dnares/9.2.47. PMID 12056414.