NUP98

Nucleoporin 98kDa
PDB rendering based on 1ko6.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1KO6​, 2Q5X​, 2Q5Y​, 3MMY​, 4OWR​
Identifiers
Symbols	NUP98 ; ADIR2; NUP196; NUP96
External IDs	OMIM: 601021 HomoloGene: 35472 GeneCards: NUP98 Gene
Gene ontology Molecular function • transporter activity • protein binding • structural constituent of nuclear pore • peptide binding Cellular component • kinetochore • nuclear envelope • nuclear pore • nucleoplasm • cytosol • nuclear pore outer ring • nuclear membrane • nuclear periphery • nuclear inclusion body • nuclear pore nuclear basket Biological process • protein import into nucleus, docking • mitotic cell cycle • carbohydrate metabolic process • DNA replication • nucleocytoplasmic transport • nuclear pore organization • mitotic nuclear envelope disassembly • small GTPase mediated signal transduction • hexose transport • gene expression • regulation of glucose transport • glucose transport • viral process • protein sumoylation • viral life cycle • viral transcription • cytokine-mediated signaling pathway • cellular response to heat • post-translational protein modification • cellular protein metabolic process • small molecule metabolic process • mRNA transport • nuclear pore complex assembly • transmembrane transport • regulation of cellular response to heat Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	4928	269966
Ensembl	ENSG00000110713	ENSMUSG00000063550
UniProt	P52948	Q6PFD9
RefSeq (mRNA)	NM_005387	NM_001287164
RefSeq (protein)	NP_005378	NP_001274093
Location (UCSC)	Chr 11: 3.67 – 3.8 Mb	Chr 7: 102.13 – 102.21 Mb
PubMed search

Nuclear pore complex protein Nup98-Nup96 is a protein that in humans is encoded by the NUP98 gene.^[1]

Function

Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is composed of approximately 50 unique proteins collectively known as nucleoporins. The 98 kD nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kD precursor protein. This cleavage results in the 98 kD nucleoporin as well as a 96 kD nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kD nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromosome translocatons in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL). This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described.^[2]

Interactions

NUP98 has been shown to interact with:

• CREB-binding protein,^[3]
• KPNB1,^[4][5]
• NUP88,^[6]
• RAE1,^[7] and
• TNPO2.^[8]

References

1. Arai Y, Hosoda F, Kobayashi H, Arai K, Hayashi Y, Kamada N, Kaneko Y, Ohki M (June 1997). "The inv(11)(p15q22) chromosome translocation of de novo and therapy-related myeloid malignancies results in fusion of the nucleoporin gene, NUP98, with the putative RNA helicase gene, DDX10". Blood 89 (11): 3936–44. PMID 9166830.
2. "Entrez Gene: NUP98 nucleoporin 98kDa".
3. Kasper LH, Brindle PK, Schnabel CA, Pritchard CE, Cleary ML, van Deursen JM (January 1999). "CREB binding protein interacts with nucleoporin-specific FG repeats that activate transcription and mediate NUP98-HOXA9 oncogenicity". Mol. Cell. Biol. 19 (1): 764–76. PMC 83933. PMID 9858599.
4. Moroianu J, Blobel G, Radu A (September 1997). "RanGTP-mediated nuclear export of karyopherin alpha involves its interaction with the nucleoporin Nup153". Proc. Natl. Acad. Sci. U.S.A. 94 (18): 9699–704. doi:10.1073/pnas.94.18.9699. PMC 23253. PMID 9275187.
5. Bonifaci N, Moroianu J, Radu A, Blobel G (May 1997). "Karyopherin beta2 mediates nuclear import of a mRNA binding protein". Proc. Natl. Acad. Sci. U.S.A. 94 (10): 5055–60. doi:10.1073/pnas.94.10.5055. PMC 24630. PMID 9144189.
6. Griffis ER, Xu S, Powers MA (February 2003). "Nup98 localizes to both nuclear and cytoplasmic sides of the nuclear pore and binds to two distinct nucleoporin subcomplexes". Mol. Biol. Cell 14 (2): 600–10. doi:10.1091/mbc.E02-09-0582. PMC 149995. PMID 12589057.
7. Pritchard CE, Fornerod M, Kasper LH, van Deursen JM (April 1999). "RAE1 is a shuttling mRNA export factor that binds to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains". J. Cell Biol. 145 (2): 237–54. doi:10.1083/jcb.145.2.237. PMC 2133102. PMID 10209021.
8. Fontoura BM, Blobel G, Yaseen NR (October 2000). "The nucleoporin Nup98 is a site for GDP/GTP exchange on ran and termination of karyopherin beta 2-mediated nuclear import". J. Biol. Chem. 275 (40): 31289–96. doi:10.1074/jbc.M004651200. PMID 10875935.

Further reading

• Slape C, Aplan PD (2004). "The role of NUP98 gene fusions in hematologic malignancy". Leuk. Lymphoma 45 (7): 1341–50. doi:10.1080/10428190310001659325. PMID 15359631.
• Moore MA, Chung KY, Plasilova M, Schuringa JJ, Shieh JH, Zhou P, Morrone G (2007). "NUP98 dysregulation in myeloid leukemogenesis". Ann. N. Y. Acad. Sci. 1106: 114–42. doi:10.1196/annals.1392.019. PMID 17442773.
• Radu A, Moore MS, Blobel G (1995). "The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex". Cell 81 (2): 215–22. doi:10.1016/0092-8674(95)90331-3. PMID 7736573.
• Nakamura T, Largaespada DA, Lee MP, Johnson LA, Ohyashiki K, Toyama K, Chen SJ, Willman CL, Chen IM, Feinberg AP, Jenkins NA, Copeland NG, Shaughnessy JD (1996). "Fusion of the nucleoporin gene NUP98 to HOXA9 by the chromosome translocation t(7;11)(p15;p15) in human myeloid leukaemia". Nat. Genet. 12 (2): 154–8. doi:10.1038/ng0296-154. PMID 8563753.
• Borrow J, Shearman AM, Stanton VP, Becher R, Collins T, Williams AJ, Dubé I, Katz F, Kwong YL, Morris C, Ohyashiki K, Toyama K, Rowley J, Housman DE (1996). "The t(7;11)(p15;p15) translocation in acute myeloid leukaemia fuses the genes for nucleoporin NUP98 and class I homeoprotein HOXA9". Nat. Genet. 12 (2): 159–67. doi:10.1038/ng0296-159. PMID 8563754.
• Hu T, Guan T, Gerace L (1996). "Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins". J. Cell Biol. 134 (3): 589–601. doi:10.1083/jcb.134.3.589. PMC 2120945. PMID 8707840.
• Stutz F, Izaurralde E, Mattaj IW, Rosbash M (1996). "A role for nucleoporin FG repeat domains in export of human immunodeficiency virus type 1 Rev protein and RNA from the nucleus". Mol. Cell. Biol. 16 (12): 7144–50. doi:10.1128/mcb.16.12.7144. PMC 231718. PMID 8943370.
• Yaseen NR, Blobel G (1997). "Cloning and characterization of human karyopherin beta3". Proc. Natl. Acad. Sci. U.S.A. 94 (9): 4451–6. doi:10.1073/pnas.94.9.4451. PMC 20743. PMID 9114010.
• Bonifaci N, Moroianu J, Radu A, Blobel G (1997). "Karyopherin beta2 mediates nuclear import of a mRNA binding protein". Proc. Natl. Acad. Sci. U.S.A. 94 (10): 5055–60. doi:10.1073/pnas.94.10.5055. PMC 24630. PMID 9144189.
• Grandi P, Dang T, Pané N, Shevchenko A, Mann M, Forbes D, Hurt E (1997). "Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly". Mol. Biol. Cell 8 (10): 2017–38. doi:10.1091/mbc.8.10.2017. PMC 25664. PMID 9348540.
• Zolotukhin AS, Felber BK (1999). "Nucleoporins nup98 and nup214 participate in nuclear export of human immunodeficiency virus type 1 Rev". J. Virol. 73 (1): 120–7. PMC 103815. PMID 9847314.
• Kasper LH, Brindle PK, Schnabel CA, Pritchard CE, Cleary ML, van Deursen JM (1999). "CREB binding protein interacts with nucleoporin-specific FG repeats that activate transcription and mediate NUP98-HOXA9 oncogenicity". Mol. Cell. Biol. 19 (1): 764–76. PMC 83933. PMID 9858599.
• Fontoura BM, Blobel G, Matunis MJ (1999). "A conserved biogenesis pathway for nucleoporins: proteolytic processing of a 186-kilodalton precursor generates Nup98 and the novel nucleoporin, Nup96". J. Cell Biol. 144 (6): 1097–112. doi:10.1083/jcb.144.6.1097. PMC 2150585. PMID 10087256.
• Pritchard CE, Fornerod M, Kasper LH, van Deursen JM (1999). "RAE1 is a shuttling mRNA export factor that binds to a GLEBS-like NUP98 motif at the nuclear pore complex through multiple domains". J. Cell Biol. 145 (2): 237–54. doi:10.1083/jcb.145.2.237. PMC 2133102. PMID 10209021.
• Ahuja HG, Felix CA, Aplan PD (1999). "The t(11;20)(p15;q11) chromosomal translocation associated with therapy-related myelodysplastic syndrome results in an NUP98-TOP1 fusion". Blood 94 (9): 3258–61. PMID 10556215.
• Bachi A, Braun IC, Rodrigues JP, Panté N, Ribbeck K, von Kobbe C, Kutay U, Wilm M, Görlich D, Carmo-Fonseca M, Izaurralde E (2000). "The C-terminal domain of TAP interacts with the nuclear pore complex and promotes export of specific CTE-bearing RNA substrates". RNA 6 (1): 136–58. doi:10.1017/S1355838200991994. PMC 1369901. PMID 10668806.
• Fontoura BM, Blobel G, Yaseen NR (2000). "The nucleoporin Nup98 is a site for GDP/GTP exchange on ran and termination of karyopherin beta 2-mediated nuclear import". J. Biol. Chem. 275 (40): 31289–96. doi:10.1074/jbc.M004651200. PMID 10875935.
• Rodrigues JP, Sitterlin D, Bachi A, Wu X, Wilm M, Carmo-Fonseca M, Izaurralde E (2000). "Vesicular stomatitis virus matrix protein inhibits host cell gene expression by targeting the nucleoporin Nup98". Mol. Cell 6 (5): 1243–52. doi:10.1016/S1097-2765(00)00120-9. PMID 11106761.
• Hofmann W, Reichart B, Ewald A, Müller E, Schmitt I, Stauber RH, Lottspeich F, Jockusch BM, Scheer U, Hauber J, Dabauvalle MC (2001). "Cofactor requirements for nuclear export of Rev response element (RRE)- and constitutive transport element (CTE)-containing retroviral RNAs. An unexpected role for actin". J. Cell Biol. 152 (5): 895–910. doi:10.1083/jcb.152.5.895. PMC 2198816. PMID 11238447.