NUTF2

Nuclear transport factor 2
PDB rendering based on 1a2k.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1GY5​
Identifiers
Symbols	NUTF2 ; NTF2; PP15
External IDs	OMIM: 605813 MGI: 1915301 HomoloGene: 110745 GeneCards: NUTF2 Gene
Gene ontology Molecular function • transporter activity • protein binding • Ran GTPase binding Cellular component • nuclear outer membrane • nuclear pore • cytosol • nuclear pore central transport channel • extracellular exosome Biological process • protein import into nucleus, translocation • protein export from nucleus • regulation of vascular endothelial growth factor production • positive regulation of protein import into nucleus Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	10204	68051
Ensembl	ENSG00000102898	ENSMUSG00000008450
UniProt	P61970	P61971
RefSeq (mRNA)	NM_005796	NM_026532
RefSeq (protein)	NP_005787	NP_080808
Location (UCSC)	Chr 16: 67.85 – 67.87 Mb	Chr 8: 105.86 – 105.88 Mb
PubMed search

Nuclear transport factor 2 is a protein that in humans is encoded by the NUTF2 gene.^[1][2][3]

Function

The protein encoded by this gene is a cytosolic factor that facilitates protein transport into the nucleus. It interacts with the nuclear pore complex glycoprotein p62. This encoded protein acts at a relative late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. It is thought to be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import.^[3]

Interactions

NUTF2 has been shown to interact with Nucleoporin 62^[1]{^[4] and RAN.^[5][6]

References

1. Paschal BM, Gerace L (Jun 1995). "Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62". J Cell Biol 129 (4): 925–937. doi:10.1083/jcb.129.4.925. PMC 2120498. PMID 7744965.
2. Grundmann U, Nerlich C, Rein T, Lottspeich F, Küpper HA (Jul 1988). "Isolation of cDNA coding for the placental protein 15 (PP15)". Nucleic Acids Res 16 (10): 4721–4721. doi:10.1093/nar/16.10.4721. PMC 336660. PMID 3380696.
3. "Entrez Gene: NUTF2 nuclear transport factor 2".
4. Hu T, Guan T, Gerace L (Aug 1996). "Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins". J. Cell Biol. 134 (3): 589–601. doi:10.1083/jcb.134.3.589. PMC 2120945. PMID 8707840.
5. Cushman I, Bowman BR, Sowa ME, Lichtarge O, Quiocho FA, Moore MS (Nov 2004). "Computational and biochemical identification of a nuclear pore complex binding site on the nuclear transport carrier NTF2". J. Mol. Biol. 344 (2): 303–310. doi:10.1016/j.jmb.2004.09.043. PMID 15522285.
6. Stewart M, Kent HM, McCoy AJ (Apr 1998). "Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran". J. Mol. Biol. 277 (3): 635–646. doi:10.1006/jmbi.1997.1602. PMID 9533885.

Further reading

• Bukrinsky MI, Haffar OK (1998). "HIV-1 nuclear import: matrix protein is back on center stage, this time together with Vpr". Mol. Med. 4 (3): 138–43. PMC 2230352. PMID 9562972.
• Stewart M (2001). "Insights into the molecular mechanism of nuclear trafficking using nuclear transport factor 2 (NTF2)". Cell Struct. Funct. 25 (4): 217–225. doi:10.1247/csf.25.217. PMID 11129791.
• Bohn H, Johannsen R, Kraus W (1981). "[New placental protein (PP15) with immunosuppressive properties (author's transl)]". Arch. Gynecol. 230 (2): 167–72. PMID 7458421.
• Hu T, Guan T, Gerace L (1996). "Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins". J. Cell Biol. 134 (3): 589–601. doi:10.1083/jcb.134.3.589. PMC 2120945. PMID 8707840.
• Bullock TL, Clarkson WD, Kent HM, Stewart M (1996). "The 1.6 angstroms resolution crystal structure of nuclear transport factor 2 (NTF2)". J. Mol. Biol. 260 (3): 422–431. doi:10.1006/jmbi.1996.0411. PMID 8757804.
• Clarkson WD, Corbett AH, Paschal BM, Kent HM, McCoy AJ, Gerace L, Silver PA, Stewart M (1997). "Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP-Ran". J. Mol. Biol. 272 (5): 716–730. doi:10.1006/jmbi.1997.1255. PMID 9368653.
• Stewart M, Kent HM, McCoy AJ (1998). "Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran". J. Mol. Biol. 277 (3): 635–646. doi:10.1006/jmbi.1997.1602. PMID 9533885.
• Ribbeck K, Lipowsky G, Kent HM, Stewart M, Görlich D (1999). "NTF2 mediates nuclear import of Ran". EMBO J. 17 (22): 6587–6598. doi:10.1093/emboj/17.22.6587. PMC 1171005. PMID 9822603.
• Steggerda SM, Black BE, Paschal BM (2000). "Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran". Mol. Biol. Cell 11 (2): 703–19. doi:10.1091/mbc.11.2.703. PMC 14804. PMID 10679025.
• Freire J, Covelo G, Sarandeses C, Díaz-Jullien C, Freire M (2001). "Identification of nuclear-import and cell-cycle regulatory proteins that bind to prothymosin alpha". Biochem. Cell Biol. 79 (2): 123–131. doi:10.1139/bcb-79-2-123. PMID 11310559.
• Cushman I, Bowman BR, Sowa ME, Lichtarge O, Quiocho FA, Moore MS (2004). "Computational and biochemical identification of a nuclear pore complex binding site on the nuclear transport carrier NTF2". J. Mol. Biol. 344 (2): 303–310. doi:10.1016/j.jmb.2004.09.043. PMID 15522285.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–1178. doi:10.1038/nature04209. PMID 16189514.

External links

• NUTF2 human gene location in the UCSC Genome Browser.
• NUTF2 human gene details in the UCSC Genome Browser.