PDS5B

PDS5 cohesin associated factor B
Identifiers
Symbols	PDS5B ; APRIN; AS3; CG008
External IDs	OMIM: 605333 MGI: 2140945 HomoloGene: 41001 GeneCards: PDS5B Gene
Gene ontology Molecular function • DNA binding • protein binding • ATP binding Cellular component • chromosome, centromeric region • chromatin • nucleus • nucleoplasm • chromosome • cytosol Biological process • mitotic cell cycle • mitotic sister chromatid cohesion • cell proliferation • negative regulation of cell proliferation • regulation of cell proliferation • cell division Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	23047	100710
Ensembl	ENSG00000083642	ENSMUSG00000034021
UniProt	Q9NTI5	Q4VA53
RefSeq (mRNA)	NM_015032	NM_175310
RefSeq (protein)	NP_055847	NP_780519
Location (UCSC)	Chr 13: 32.59 – 32.78 Mb	Chr 5: 150.67 – 150.81 Mb
PubMed search

Sister chromatid cohesion protein PDS5 homolog B is a protein that in humans is encoded by the PDS5B gene.^[1][2][3]

Model organisms

Pds5b knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Abnormal
Recessive lethal study	Abnormal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology	Normal
Peripheral blood lymphocytes	Normal
Heart weight	Normal
Skin Histopathology	Normal
Brain histopathology	Normal
Citrobacter infection	Normal[4]
All tests and analysis from[5][6]

Model organisms have been used in the study of PDS5B function. A conditional knockout mouse line, called Pds5b^{tm1a(EUCOMM)Wtsi[7][8]} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists — at the Wellcome Trust Sanger Institute.^[9][10][11]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.^[5][12] Twenty three tests were carried out and two phenotypes were reported. Almost all homozygous mutant animals died prior to birth, and therefore they did not survive until weaning. The remaining tests were carried out on heterozygous mutant mice, and no significant abnormalities were observed. ^[5]

References

1. Couch FJ, Rommens JM, Neuhausen SL, Belanger C, Dumont M, Abel K, Bell R, Berry S, Bogden R, Cannon-Albright L, Farid L, Frye C, Hattier T, Janecki T, Jiang P, Kehrer R, Leblanc JF, McArthur-Morrison J, Meney D, Miki Y, Peng Y, Samson C, Schroeder M, Snyder SC, Simard J; et al. (Feb 1997). "Generation of an integrated transcription map of the BRCA2 region on chromosome 13q12-q13". Genomics 36 (1): 86–99. doi:10.1006/geno.1996.0428. PMID 8812419.
2. Geck P, Szelei J, Jimenez J, Sonnenschein C, Soto AM (May 1999). "Early gene expression during androgen-induced inhibition of proliferation of prostate cancer cells: a new suppressor candidate on chromosome 13, in the BRCA2-Rb1 locus". J Steroid Biochem Mol Biol 68 (1–2): 41–50. doi:10.1016/S0960-0760(98)00165-4. PMID 10215036.
3. "Entrez Gene: APRIN androgen-induced proliferation inhibitor".
4. "Citrobacter infection data for Pds5b". Wellcome Trust Sanger Institute.
5. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88 (S248). doi:10.1111/j.1755-3768.2010.4142.x.
6. Mouse Resources Portal, Wellcome Trust Sanger Institute.
7. "International Knockout Mouse Consortium".
8. "Mouse Genome Informatics".
9. Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
10. Dolgin E (June 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
11. Collins FS, Rossant J, Wurst W (January 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
12. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

Further reading

• Nagase T, Ishikawa K, Suyama M, et al. (1999). "Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 6 (1): 63–70. doi:10.1093/dnares/6.1.63. PMID 10231032.
• Geck P, Maffini MV, Szelei J, et al. (2000). "Androgen-induced proliferative quiescence in prostate cancer cells: the role of AS3 as its mediator". Proc. Natl. Acad. Sci. U.S.A. 97 (18): 10185–90. doi:10.1073/pnas.97.18.10185. PMC 27796. PMID 10963680.
• Maffini MV, Geck P, Powell CE, et al. (2002). "Mechanism of androgen action on cell proliferation: AS3 protein as a mediator of proliferative arrest in the rat prostate". Endocrinology 143 (7): 2708–14. doi:10.1210/en.143.7.2708. PMID 12072405.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Ballif BA, Villén J, Beausoleil SA, et al. (2005). "Phosphoproteomic analysis of the developing mouse brain". Mol. Cell Proteomics 3 (11): 1093–101. doi:10.1074/mcp.M400085-MCP200. PMID 15345747.
• Rankin S, Ayad NG, Kirschner MW (2005). "Sororin, a substrate of the anaphase-promoting complex, is required for sister chromatid cohesion in vertebrates". Mol. Cell 18 (2): 185–200. doi:10.1016/j.molcel.2005.03.017. PMID 15837422.
• Losada A, Yokochi T, Hirano T (2006). "Functional contribution of Pds5 to cohesin-mediated cohesion in human cells and Xenopus egg extracts". J. Cell. Sci. 118 (Pt 10): 2133–41. doi:10.1242/jcs.02355. PMID 15855230.