PEX14

Peroxisomal biogenesis factor 14
Rendering based on PDB 2W84​.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2W84​, 2W85​, 4BXU​
Identifiers
Symbols	PEX14 ; NAPP2; PBD13A; Pex14p; dJ734G22.2
External IDs	OMIM: 601791 MGI: 1927868 HomoloGene: 37936 GeneCards: PEX14 Gene
Gene ontology Molecular function • transcription corepressor activity • receptor binding • protein binding • microtubule binding • protein N-terminus binding • beta-tubulin binding Cellular component • intracellular • nucleus • nucleolus • peroxisome • peroxisomal membrane • membrane • integral component of membrane • intracellular membrane-bounded organelle • protein complex Biological process • protein complex assembly • peroxisome organization • protein import into peroxisome matrix • protein import into peroxisome matrix, translocation • negative regulation of protein binding • microtubule anchoring • peroxisome transport along microtubule • negative regulation of sequence-specific DNA binding transcription factor activity • protein import into peroxisome matrix, substrate release • negative regulation of transcription, DNA-templated • protein homooligomerization • negative regulation of protein homotetramerization Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	5195	56273
Ensembl	ENSG00000142655	ENSMUSG00000028975
UniProt	O75381	Q9R0A0
RefSeq (mRNA)	NM_004565	NM_019781
RefSeq (protein)	NP_004556	NP_062755
Location (UCSC)	Chr 1: 10.47 – 10.63 Mb	Chr 4: 148.96 – 149.1 Mb
PubMed search

Peroxisomal membrane anchor protein (Pex14p) conserved region
Identifiers
Symbol	Pex14_N
Pfam	PF04695
InterPro	IPR006785
Available protein structures: Pfam structures PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Peroxisomal membrane protein PEX14 is a protein that in humans is encoded by the PEX14 gene.^[1][2]

Function

This gene encodes an essential component of the peroxisomal import machinery. The protein is integrated into peroxisome membranes with its C-terminus exposed to the cytosol, and interacts with the cytosolic receptor for proteins containing a PTS1 peroxisomal targeting signal. The protein also functions as a transcriptional corepressor and interacts with a histone deacetylase. A mutation in this gene results in one form of Zellweger syndrome.^[2]

Interactions

PEX14 has been shown to interact with

• PEX5,^[3][4][5][6]
• PEX7,^[6] and
• PEX13.^[3]

References

1. Fransen M, Terlecky SR, Subramani S (Jul 1998). "Identification of a human PTS1 receptor docking protein directly required for peroxisomal protein import". Proceedings of the National Academy of Sciences of the United States of America 95 (14): 8087–92. doi:10.1073/pnas.95.14.8087. PMC 20933. PMID 9653144.
2. "Entrez Gene: PEX14 peroxisomal biogenesis factor 14".
3. Otera H, Setoguchi K, Hamasaki M, Kumashiro T, Shimizu N, Fujiki Y (Mar 2002). "Peroxisomal targeting signal receptor Pex5p interacts with cargoes and import machinery components in a spatiotemporally differentiated manner: conserved Pex5p WXXXF/Y motifs are critical for matrix protein import". Molecular and Cellular Biology 22 (6): 1639–55. doi:10.1128/MCB.22.6.1639-1655.2002. PMC 135613. PMID 11865044.
4. Okumoto K, Abe I, Fujiki Y (Aug 2000). "Molecular anatomy of the peroxin Pex12p: ring finger domain is essential for Pex12p function and interacts with the peroxisome-targeting signal type 1-receptor Pex5p and a ring peroxin, Pex10p". The Journal of Biological Chemistry 275 (33): 25700–10. doi:10.1074/jbc.M003303200. PMID 10837480.
5. Shimizu N, Itoh R, Hirono Y, Otera H, Ghaedi K, Tateishi K, Tamura S, Okumoto K, Harano T, Mukai S, Fujiki Y (Apr 1999). "The peroxin Pex14p. cDNA cloning by functional complementation on a Chinese hamster ovary cell mutant, characterization, and functional analysis". The Journal of Biological Chemistry 274 (18): 12593–604. doi:10.1074/jbc.274.18.12593. PMID 10212238.
6. Managadze D, Würtz C, Sichting M, Niehaus G, Veenhuis M, Rottensteiner H (Jun 2007). "The peroxin PEX14 of Neurospora crassa is essential for the biogenesis of both glyoxysomes and Woronin bodies". Traffic 8 (6): 687–701. doi:10.1111/j.1600-0854.2007.00560.x. PMID 17461798.

Further reading

• Albertini M, Rehling P, Erdmann R, Girzalsky W, Kiel JA, Veenhuis M, Kunau WH (Apr 1997). "Pex14p, a peroxisomal membrane protein binding both receptors of the two PTS-dependent import pathways". Cell 89 (1): 83–92. doi:10.1016/S0092-8674(00)80185-3. PMID 9094717.
• Huhse B, Rehling P, Albertini M, Blank L, Meller K, Kunau WH (Jan 1998). "Pex17p of Saccharomyces cerevisiae is a novel peroxin and component of the peroxisomal protein translocation machinery". The Journal of Cell Biology 140 (1): 49–60. doi:10.1083/jcb.140.1.49. PMC 2132588. PMID 9425153.
• Will GK, Soukupova M, Hong X, Erdmann KS, Kiel JA, Dodt G, Kunau WH, Erdmann R (Mar 1999). "Identification and characterization of the human orthologue of yeast Pex14p". Molecular and Cellular Biology 19 (3): 2265–77. doi:10.1128/mcb.19.3.2265. PMC 84019. PMID 10022913.
• Girzalsky W, Rehling P, Stein K, Kipper J, Blank L, Kunau WH, Erdmann R (Mar 1999). "Involvement of Pex13p in Pex14p localization and peroxisomal targeting signal 2-dependent protein import into peroxisomes". The Journal of Cell Biology 144 (6): 1151–62. doi:10.1083/jcb.144.6.1151. PMC 2150583. PMID 10087260.
• Shimizu N, Itoh R, Hirono Y, Otera H, Ghaedi K, Tateishi K, Tamura S, Okumoto K, Harano T, Mukai S, Fujiki Y (Apr 1999). "The peroxin Pex14p. cDNA cloning by functional complementation on a Chinese hamster ovary cell mutant, characterization, and functional analysis". The Journal of Biological Chemistry 274 (18): 12593–604. doi:10.1074/jbc.274.18.12593. PMID 10212238.
• Sacksteder KA, Jones JM, South ST, Li X, Liu Y, Gould SJ (Mar 2000). "PEX19 binds multiple peroxisomal membrane proteins, is predominantly cytoplasmic, and is required for peroxisome membrane synthesis". The Journal of Cell Biology 148 (5): 931–44. doi:10.1083/jcb.148.5.931. PMC 2174547. PMID 10704444.
• Gavva NR, Wen SC, Daftari P, Moniwa M, Yang WM, Yang-Feng LP, Seto E, Davie JR, Shen CK (Mar 2002). "NAPP2, a peroxisomal membrane protein, is also a transcriptional corepressor". Genomics 79 (3): 423–31. doi:10.1006/geno.2002.6714. PMID 11863372.
• Otera H, Setoguchi K, Hamasaki M, Kumashiro T, Shimizu N, Fujiki Y (Mar 2002). "Peroxisomal targeting signal receptor Pex5p interacts with cargoes and import machinery components in a spatiotemporally differentiated manner: conserved Pex5p WXXXF/Y motifs are critical for matrix protein import". Molecular and Cellular Biology 22 (6): 1639–55. doi:10.1128/MCB.22.6.1639-1655.2002. PMC 135613. PMID 11865044.
• Alland L, David G, Shen-Li H, Potes J, Muhle R, Lee HC, Hou H, Chen K, DePinho RA (Apr 2002). "Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex". Molecular and Cellular Biology 22 (8): 2743–50. doi:10.1128/MCB.22.8.2743-2750.2002. PMC 133736. PMID 11909966.
• Fransen M, Brees C, Ghys K, Amery L, Mannaerts GP, Ladant D, Van Veldhoven PP (Mar 2002). "Analysis of mammalian peroxin interactions using a non-transcription-based bacterial two-hybrid assay". Molecular & Cellular Proteomics 1 (3): 243–52. doi:10.1074/mcp.M100025-MCP200. PMID 12096124.
• Oliveira ME, Reguenga C, Gouveia AM, Guimarães CP, Schliebs W, Kunau WH, Silva MT, Sá-Miranda C, Azevedo JE (Dec 2002). "Mammalian Pex14p: membrane topology and characterisation of the Pex14p-Pex14p interaction". Biochimica Et Biophysica Acta 1567 (1-2): 13–22. doi:10.1016/s0005-2736(02)00635-1. PMID 12488033.
• Fransen M, Vastiau I, Brees C, Brys V, Mannaerts GP, Van Veldhoven PP (Mar 2004). "Potential role for Pex19p in assembly of PTS-receptor docking complexes". The Journal of Biological Chemistry 279 (13): 12615–24. doi:10.1074/jbc.M304941200. PMID 14715663.
• Shimozawa N, Tsukamoto T, Nagase T, Takemoto Y, Koyama N, Suzuki Y, Komori M, Osumi T, Jeannette G, Wanders RJ, Kondo N (Jun 2004). "Identification of a new complementation group of the peroxisome biogenesis disorders and PEX14 as the mutated gene". Human Mutation 23 (6): 552–8. doi:10.1002/humu.20032. PMID 15146459.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S (Jan 2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Research 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
• Nguyen T, Bjorkman J, Paton BC, Crane DI (Feb 2006). "Failure of microtubule-mediated peroxisome division and trafficking in disorders with reduced peroxisome abundance". Journal of Cell Science 119 (Pt 4): 636–45. doi:10.1242/jcs.02776. PMID 16449325.

External links

• GeneReviews/NCBI/NIH/UW entry on Peroxisome Biogenesis Disorders, Zellweger Syndrome Spectrum
• OMIM entries on Peroxisome Biogenesis Disorders, Zellweger Syndrome Spectrum
• Eukaryotic Linear Motif resource motif class TRG_PEX_1
• Eukaryotic Linear Motif resource motif class TRG_PEX_2