PIK3C2B

Phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 beta
Identifiers
Symbols	PIK3C2B ; C2-PI3K
External IDs	OMIM: 602838 MGI: 2685045 HomoloGene: 20582 ChEMBL: 5554 GeneCards: PIK3C2B Gene
EC number	2.7.1.154
Gene ontology Molecular function • lipid kinase activity • protein binding • ATP binding • 1-phosphatidylinositol-3-kinase activity • 1-phosphatidylinositol-4-phosphate 3-kinase activity • phosphatidylinositol binding Cellular component • nucleus • cytoplasm • endoplasmic reticulum • cytosol • plasma membrane • phosphatidylinositol 3-kinase complex • endocytic vesicle Biological process • adaptive immune response • protein phosphorylation • phospholipid metabolic process • phosphatidylinositol biosynthetic process • inflammatory response • biological_process • phosphatidylinositol 3-kinase signaling • platelet activation • phosphatidylinositol-3-phosphate biosynthetic process • protein kinase B signaling • small molecule metabolic process • innate immune response • phosphatidylinositol phosphorylation • cell chemotaxis Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	5287	240752
Ensembl	ENSG00000133056	ENSMUSG00000026447
UniProt	O00750	E9QAN8
RefSeq (mRNA)	NM_002646	NM_001099276
RefSeq (protein)	NP_002637	NP_001092746
Location (UCSC)	Chr 1: 204.42 – 204.49 Mb	Chr 1: 133.05 – 133.11 Mb
PubMed search

Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing beta polypeptide is an enzyme that in humans is encoded by the PIK3C2B gene.^[1][2][3]

Function

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmannin. The C2 domain of this protein was shown to bind phospholipids but not Ca²⁺, which suggests that this enzyme may function in a calcium-independent manner.^[3]

References

1. Brown RA, Ho LK, Weber-Hall SJ, Shipley JM, Fry MJ (Apr 1997). "Identification and cDNA cloning of a novel mammalian C2 domain-containing phosphoinositide 3-kinase, HsC2-PI3K". Biochemical and Biophysical Research Communications 233 (2): 537–44. doi:10.1006/bbrc.1997.6495. PMID 9144573.
2. Arcaro A, Volinia S, Zvelebil MJ, Stein R, Watton SJ, Layton MJ, Gout I, Ahmadi K, Downward J, Waterfield MD (Dec 1998). "Human phosphoinositide 3-kinase C2beta, the role of calcium and the C2 domain in enzyme activity". The Journal of Biological Chemistry 273 (49): 33082–90. doi:10.1074/jbc.273.49.33082. PMID 9830063.
3. "Entrez Gene: PIK3C2B phosphoinositide-3-kinase, class 2, beta polypeptide".

Further reading

• Lee C, Liu QH, Tomkowicz B, Yi Y, Freedman BD, Collman RG (Nov 2003). "Macrophage activation through CCR5- and CXCR4-mediated gp120-elicited signaling pathways". Journal of Leukocyte Biology 74 (5): 676–82. doi:10.1189/jlb.0503206. PMID 12960231.
• Kozutsumi H, Toyoshima H, Hagiwara K, Yazaki Y, Hirai H (Oct 1994). "Human ltk receptor tyrosine kinase binds to PLC-gamma 1, PI3-K, GAP and Raf-1 in vivo". Oncogene 9 (10): 2991–8. PMID 8084603.
• Milani D, Mazzoni M, Borgatti P, Zauli G, Cantley L, Capitani S (Sep 1996). "Extracellular human immunodeficiency virus type-1 Tat protein activates phosphatidylinositol 3-kinase in PC12 neuronal cells". The Journal of Biological Chemistry 271 (38): 22961–4. doi:10.1074/jbc.271.38.22961. PMID 8798481.
• Ueno H, Honda H, Nakamoto T, Yamagata T, Sasaki K, Miyagawa K, Mitani K, Yazaki Y, Hirai H (Jun 1997). "The phosphatidylinositol 3' kinase pathway is required for the survival signal of leukocyte tyrosine kinase". Oncogene 14 (25): 3067–72. doi:10.1038/sj.onc.1201153. PMID 9223670.
• Mazerolles F, Barbat C, Fischer A (Sep 1997). "Down-regulation of LFA-1-mediated T cell adhesion induced by the HIV envelope glycoprotein gp160 requires phosphatidylinositol-3-kinase activity". European Journal of Immunology 27 (9): 2457–65. doi:10.1002/eji.1830270946. PMID 9341793.
• Borgatti P, Zauli G, Colamussi ML, Gibellini D, Previati M, Cantley LL, Capitani S (Nov 1997). "Extracellular HIV-1 Tat protein activates phosphatidylinositol 3- and Akt/PKB kinases in CD4+ T lymphoblastoid Jurkat cells". European Journal of Immunology 27 (11): 2805–11. doi:10.1002/eji.1830271110. PMID 9394803.
• Borgatti P, Zauli G, Cantley LC, Capitani S (Jan 1998). "Extracellular HIV-1 Tat protein induces a rapid and selective activation of protein kinase C (PKC)-alpha, and -epsilon and -zeta isoforms in PC12 cells". Biochemical and Biophysical Research Communications 242 (2): 332–7. doi:10.1006/bbrc.1997.7877. PMID 9446795.
• Milani D, Mazzoni M, Zauli G, Mischiati C, Gibellini D, Giacca M, Capitani S (Jul 1998). "HIV-1 Tat induces tyrosine phosphorylation of p125FAK and its association with phosphoinositide 3-kinase in PC12 cells". Aids 12 (11): 1275–84. doi:10.1097/00002030-199811000-00008. PMID 9708406.
• Jauliac S, Mazerolles F, Jabado N, Pallier A, Bernard F, Peake J, Fischer A, Hivroz C (Oct 1998). "Ligands of CD4 inhibit the association of phospholipase Cgamma1 with phosphoinositide 3 kinase in T cells: regulation of this association by the phosphoinositide 3 kinase activity". European Journal of Immunology 28 (10): 3183–91. doi:10.1002/(SICI)1521-4141(199810)28:10<3183::AID-IMMU3183>3.0.CO;2-A. PMID 9808187.
• Arcaro A, Zvelebil MJ, Wallasch C, Ullrich A, Waterfield MD, Domin J (Jun 2000). "Class II phosphoinositide 3-kinases are downstream targets of activated polypeptide growth factor receptors". Molecular and Cellular Biology 20 (11): 3817–30. doi:10.1128/MCB.20.11.3817-3830.2000. PMC 85707. PMID 10805725.
• Park IW, Wang JF, Groopman JE (Jan 2001). "HIV-1 Tat promotes monocyte chemoattractant protein-1 secretion followed by transmigration of monocytes". Blood 97 (2): 352–8. doi:10.1182/blood.V97.2.352. PMID 11154208.
• Zauli G, Milani D, Mirandola P, Mazzoni M, Secchiero P, Miscia S, Capitani S (Feb 2001). "HIV-1 Tat protein down-regulates CREB transcription factor expression in PC12 neuronal cells through a phosphatidylinositol 3-kinase/AKT/cyclic nucleoside phosphodiesterase pathway". FASEB Journal 15 (2): 483–91. doi:10.1096/fj.00-0354com. PMID 11156964.
• Wheeler M, Domin J (Oct 2001). "Recruitment of the class II phosphoinositide 3-kinase C2beta to the epidermal growth factor receptor: role of Grb2". Molecular and Cellular Biology 21 (19): 6660–7. doi:10.1128/MCB.21.19.6660-6667.2001. PMC 99811. PMID 11533253.
• Deregibus MC, Cantaluppi V, Doublier S, Brizzi MF, Deambrosis I, Albini A, Camussi G (Jul 2002). "HIV-1-Tat protein activates phosphatidylinositol 3-kinase/ AKT-dependent survival pathways in Kaposi's sarcoma cells". The Journal of Biological Chemistry 277 (28): 25195–202. doi:10.1074/jbc.M200921200. PMID 11994280.
• Cook JA, August A, Henderson AJ (Jul 2002). "Recruitment of phosphatidylinositol 3-kinase to CD28 inhibits HIV transcription by a Tat-dependent mechanism". Journal of Immunology 169 (1): 254–60. doi:10.4049/jimmunol.169.1.254. PMID 12077252.
• Arcaro A, Khanzada UK, Vanhaesebroeck B, Tetley TD, Waterfield MD, Seckl MJ (Oct 2002). "Two distinct phosphoinositide 3-kinases mediate polypeptide growth factor-stimulated PKB activation". The EMBO Journal 21 (19): 5097–108. doi:10.1093/emboj/cdf512. PMC 129034. PMID 12356726.
• Visnjić D, Crljen V, Curić J, Batinić D, Volinia S, Banfić H (Oct 2002). "The activation of nuclear phosphoinositide 3-kinase C2beta in all-trans-retinoic acid-differentiated HL-60 cells". FEBS Letters 529 (2-3): 268–74. doi:10.1016/S0014-5793(02)03357-4. PMID 12372612.