PIP5K1C

Phosphatidylinositol-4-phosphate 5-kinase, type I, gamma
Rendering based on PDB 3H1Z​.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2G35​, 3H1Z​, 3H85​
Identifiers
Symbols	PIP5K1C ; LCCS3; PIP5K-GAMMA; PIP5K1-gamma; PIP5Kgamma
External IDs	OMIM: 606102 MGI: 1298224 HomoloGene: 69032 GeneCards: PIP5K1C Gene
EC number	2.7.1.68
Gene ontology Molecular function • protein binding • ATP binding • 1-phosphatidylinositol-4-phosphate 5-kinase activity • talin binding Cellular component • phagocytic cup • uropod • nucleus • cytosol • focal adhesion • endomembrane system • ruffle membrane Biological process • phospholipid metabolic process • phosphatidylinositol biosynthetic process • phagocytosis • cytoskeletal anchoring at plasma membrane • axon guidance • synaptic vesicle exocytosis • single organismal cell-cell adhesion • actin cytoskeleton organization • neutrophil chemotaxis • adherens junction assembly • small molecule metabolic process • phosphatidylinositol phosphorylation • synaptic vesicle endocytosis • platelet aggregation • clathrin-mediated endocytosis Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	23396	18717
Ensembl	ENSG00000186111	ENSMUSG00000034902
UniProt	O60331	O70161
RefSeq (mRNA)	NM_001195733	NM_001146687
RefSeq (protein)	NP_001182662	NP_001140159
Location (UCSC)	Chr 19: 3.63 – 3.7 Mb	Chr 10: 81.29 – 81.32 Mb
PubMed search

Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma is an enzyme that in humans is encoded by the PIP5K1C gene.^[1][2]

This gene encodes a member of the type I phosphatidylinositol-4-phosphate 5-kinase family of enzymes. A similar protein in mice is found in synapses and focal adhesion plaques, and binds the FERM domain of talin through its C-terminus.^[2]

Model organisms

Pip5k1c knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Abnormal
Recessive lethal study	Abnormal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology	Normal
Peripheral blood lymphocytes	Normal
Micronucleus test	Normal
Heart weight	Normal
Salmonella infection	Normal[3]
Citrobacter infection	Normal[4]
All tests and analysis from[5][6]

Model organisms have been used in the study of PIP5K1C function. A conditional knockout mouse line, called Pip5k1c^{tm1a(KOMP)Wtsi[7][8]} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.^[9][10][11]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.^[5][12] Twenty three tests were carried out on mutant mice and two significant abnormalities were observed.^[5] Fewer than expected homozygous mutant embryos were identified during gestation, and none survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice and no further phenotypes were observed.^[5]

References

1. Ishihara H, Shibasaki Y, Kizuki N, Wada T, Yazaki Y, Asano T, Oka Y (May 1998). "Type I phosphatidylinositol-4-phosphate 5-kinases. Cloning of the third isoform and deletion/substitution analysis of members of this novel lipid kinase family". J Biol Chem 273 (15): 8741–8. doi:10.1074/jbc.273.15.8741. PMID 9535851.
2. "Entrez Gene: PIP5K1C phosphatidylinositol-4-phosphate 5-kinase, type I, gamma".
3. "Salmonella infection data for Pip5k1c". Wellcome Trust Sanger Institute.
4. "Citrobacter infection data for Pip5k1c". Wellcome Trust Sanger Institute.
5. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
6. Mouse Resources Portal, Wellcome Trust Sanger Institute.
7. "International Knockout Mouse Consortium".
8. "Mouse Genome Informatics".
9. Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
10. Dolgin E (2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
11. Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
12. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.

Further reading

• Niiro H, Clark EA (2003). "Branches of the B cell antigen receptor pathway are directed by protein conduits Bam32 and Carma1.". Immunity 19 (5): 637–40. doi:10.1016/S1074-7613(03)00303-0. PMID 14614850.
• Carpenter CL (2004). "Btk-dependent regulation of phosphoinositide synthesis.". Biochem. Soc. Trans. 32 (Pt 2): 326–9. doi:10.1042/BST0320326. PMID 15046600.
• Nagase T, Ishikawa K, Miyajima N, et al. (1998). "Prediction of the coding sequences of unidentified human genes. IX. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro.". DNA Res. 5 (1): 31–9. doi:10.1093/dnares/5.1.31. PMID 9628581.
• Gotthardt M, Trommsdorff M, Nevitt MF, et al. (2000). "Interactions of the low density lipoprotein receptor gene family with cytosolic adaptor and scaffold proteins suggest diverse biological functions in cellular communication and signal transduction.". J. Biol. Chem. 275 (33): 25616–24. doi:10.1074/jbc.M000955200. PMID 10827173.
• Wenk MR, Pellegrini L, Klenchin VA, et al. (2001). "PIP kinase Igamma is the major PI(4,5)P(2) synthesizing enzyme at the synapse.". Neuron 32 (1): 79–88. doi:10.1016/S0896-6273(01)00456-1. PMID 11604140.
• Di Paolo G, Pellegrini L, Letinic K, et al. (2002). "Recruitment and regulation of phosphatidylinositol phosphate kinase type 1 gamma by the FERM domain of talin.". Nature 420 (6911): 85–9. doi:10.1038/nature01147. PMID 12422219.
• Ling K, Doughman RL, Firestone AJ, et al. (2002). "Type I gamma phosphatidylinositol phosphate kinase targets and regulates focal adhesions.". Nature 420 (6911): 89–93. doi:10.1038/nature01082. PMID 12422220.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Doughman RL, Firestone AJ, Wojtasiak ML, et al. (2003). "Membrane ruffling requires coordination between type Ialpha phosphatidylinositol phosphate kinase and Rac signaling.". J. Biol. Chem. 278 (25): 23036–45. doi:10.1074/jbc.M211397200. PMID 12682053.
• Krauss M, Kinuta M, Wenk MR, et al. (2003). "ARF6 stimulates clathrin/AP-2 recruitment to synaptic membranes by activating phosphatidylinositol phosphate kinase type Igamma.". J. Cell Biol. 162 (1): 113–24. doi:10.1083/jcb.200301006. PMC 2172713. PMID 12847086.
• Chang JD, Field SJ, Rameh LE, et al. (2004). "Identification and characterization of a phosphoinositide phosphate kinase homolog.". J. Biol. Chem. 279 (12): 11672–9. doi:10.1074/jbc.M309721200. PMID 14701839.
• Grimwood J, Gordon LA, Olsen A, et al. (2004). "The DNA sequence and biology of human chromosome 19.". Nature 428 (6982): 529–35. doi:10.1038/nature02399. PMID 15057824.
• Wang YJ, Li WH, Wang J, et al. (2005). "Critical role of PIP5KI{gamma}87 in InsP3-mediated Ca(2+) signaling.". J. Cell Biol. 167 (6): 1005–10. doi:10.1083/jcb.200408008. PMC 2172614. PMID 15611330.
• Bairstow SF, Ling K, Su X, et al. (2006). "Type Igamma661 phosphatidylinositol phosphate kinase directly interacts with AP2 and regulates endocytosis.". J. Biol. Chem. 281 (29): 20632–42. doi:10.1074/jbc.M601465200. PMID 16707488.
• Krauss M, Kukhtina V, Pechstein A, Haucke V (2006). "Stimulation of phosphatidylinositol kinase type I-mediated phosphatidylinositol (4,5)-bisphosphate synthesis by AP-2mu-cargo complexes.". Proc. Natl. Acad. Sci. U.S.A. 103 (32): 11934–9. doi:10.1073/pnas.0510306103. PMC 1567676. PMID 16880396.
• Auvinen E, Kivi N, Vaheri A (2007). "Regulation of ezrin localization by Rac1 and PIPK in human epithelial cells.". Exp. Cell Res. 313 (4): 824–33. doi:10.1016/j.yexcr.2006.12.002. PMID 17229424.
• Ling K, Bairstow SF, Carbonara C, et al. (2007). "Type Igamma phosphatidylinositol phosphate kinase modulates adherens junction and E-cadherin trafficking via a direct interaction with mu 1B adaptin.". J. Cell Biol. 176 (3): 343–53. doi:10.1083/jcb.200606023. PMC 2063960. PMID 17261850.