POLQ

Polymerase (DNA directed), theta
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 4X0P​, 4X0Q​, 5AGA​
Identifiers
Symbols	POLQ ; POLH; PRO0327
External IDs	OMIM: 604419 MGI: 2155399 HomoloGene: 32727 ChEMBL: 6025 GeneCards: POLQ Gene
EC number	2.7.7.7
Gene ontology Molecular function • chromatin binding • damaged DNA binding • DNA-directed DNA polymerase activity • protein binding • ATP binding • single-stranded DNA-dependent ATPase activity • 5'-deoxyribose-5-phosphate lyase activity Cellular component • nucleoplasm • chromosome Biological process • DNA-dependent DNA replication • DNA repair • base-excision repair • double-strand break repair • cellular response to DNA damage stimulus • somatic hypermutation of immunoglobulin genes • protein homooligomerization • DNA biosynthetic process • double-strand break repair via alternative nonhomologous end joining • negative regulation of double-strand break repair via homologous recombination Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	10721	77782
Ensembl	ENSG00000051341	ENSMUSG00000034206
UniProt	O75417	Q8CGS6
RefSeq (mRNA)	NM_199420	NM_001159369
RefSeq (protein)	NP_955452	NP_001152841
Location (UCSC)	Chr 3: 121.43 – 121.55 Mb	Chr 16: 37.01 – 37.1 Mb
PubMed search

DNA polymerase theta is an enzyme that in humans is encoded by the POLQ gene.^[1][2]

References

1. Sharief FS, Vojta PJ, Ropp PA, Copeland WC (Aug 1999). "Cloning and chromosomal mapping of the human DNA polymerase theta (POLQ), the eighth human DNA polymerase". Genomics 59 (1): 90–6. doi:10.1006/geno.1999.5843. PMID 10395804.
2. "Entrez Gene: POLQ polymerase (DNA directed), theta".

Further reading

• Hogg M, Sauer-Eriksson AE, Johansson E (2012). "Promiscuous DNA synthesis by human DNA polymerase θ.". Nucleic Acids Research 40 (6): 2611–2622. doi:10.1093/nar/gkr1102.
• Robertson NG, Khetarpal U, Gutiérrez-Espeleta GA, et al. (1995). "Isolation of novel and known genes from a human fetal cochlear cDNA library using subtractive hybridization and differential screening.". Genomics 23 (1): 42–50. doi:10.1006/geno.1994.1457. PMID 7829101.
• Maga G, Shevelev I, Ramadan K, et al. (2002). "DNA polymerase theta purified from human cells is a high-fidelity enzyme.". J. Mol. Biol. 319 (2): 359–69. doi:10.1016/S0022-2836(02)00325-X. PMID 12051913.
• Seki M, Marini F, Wood RD (2003). "POLQ (Pol θ), a DNA polymerase and DNA-dependent ATPase in human cells". Nucleic Acids Res. 31 (21): 6117–26. doi:10.1093/nar/gkg814. PMC 275456. PMID 14576298.
• Kawamura K, Bahar R, Seimiya M, et al. (2004). "DNA polymerase theta is preferentially expressed in lymphoid tissues and upregulated in human cancers". Int. J. Cancer 109 (1): 9–16. doi:10.1002/ijc.11666. PMID 14735462.
• Suzuki Y, Yamashita R, Shirota M, et al. (2004). "Sequence Comparison of Human and Mouse Genes Reveals a Homologous Block Structure in the Promoter Regions". Genome Res. 14 (9): 1711–8. doi:10.1101/gr.2435604. PMC 515316. PMID 15342556.
• Seki M, Masutani C, Yang LW, et al. (2005). "High-efficiency bypass of DNA damage by human DNA polymerase Q". EMBO J. 23 (22): 4484–94. doi:10.1038/sj.emboj.7600424. PMC 526458. PMID 15496986.
• Chiapperino D, Cai M, Sayer JM, et al. (2006). "Error-prone translesion synthesis by human DNA polymerase eta on DNA-containing deoxyadenosine adducts of 7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene". J. Biol. Chem. 280 (48): 39684–92. doi:10.1074/jbc.M508008200. PMID 16188888.
• Zan H, Shima N, Xu Z, et al. (2005). "The translesion DNA polymerase θ plays a dominant role in immunoglobulin gene somatic hypermutation". EMBO J. 24 (21): 3757–69. doi:10.1038/sj.emboj.7600833. PMC 1276717. PMID 16222339.
• Cruet-Hennequart S, Coyne S, Glynn MT, et al. (2006). "UV-induced RPA phosphorylation is increased in the absence of DNA polymerase eta and requires DNA-PK". DNA Repair (Amst.) 5 (4): 491–504. doi:10.1016/j.dnarep.2006.01.008. PMID 16520097.
• Chen YW, Cleaver JE, Hanaoka F, et al. (2006). "A novel role of DNA polymerase eta in modulating cellular sensitivity to chemotherapeutic agents". Mol. Cancer Res. 4 (4): 257–65. doi:10.1158/1541-7786.MCR-05-0118. PMID 16603639.
• Yuasa MS, Masutani C, Hirano A, et al. (2006). "A human DNA polymerase eta complex containing Rad18, Rad6 and Rev1; proteomic analysis and targeting of the complex to the chromatin-bound fraction of cells undergoing replication fork arrest". Genes Cells 11 (7): 731–44. doi:10.1111/j.1365-2443.2006.00974.x. PMID 16824193.
• Choi JY, Stover JS, Angel KC, et al. (2006). "Biochemical basis of genotoxicity of heterocyclic arylamine food mutagens: Human DNA polymerase eta selectively produces a two-base deletion in copying the N2-guanyl adduct of 2-amino-3-methylimidazo[4,5-f]quinoline but not the C8 adduct at the NarI G3 site". J. Biol. Chem. 281 (35): 25297–306. doi:10.1074/jbc.M605699200. PMID 16835218.
• Kino K, Ito N, Sugasawa K, et al. (2007). "Translesion synthesis by human DNA polymerase eta across oxidative products of guanine". Nucleic Acids Symp Ser (Oxf) 48 (48): 171–2. doi:10.1093/nass/48.1.171. PMID 17150533.
• Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.