POLR1E

Polymerase (RNA) I polypeptide E, 53kDa
Identifiers
Symbols	POLR1E ; PAF53; PRAF1
External IDs	MGI: 1929022 HomoloGene: 41486 GeneCards: POLR1E Gene
Gene ontology Molecular function • RNA polymerase I activity • RNA polymerase I transcription factor binding • DNA binding Cellular component • nucleus • nucleoplasm • nucleolus • DNA-directed RNA polymerase I complex Biological process • RNA polymerase I transcriptional preinitiation complex assembly at the promoter for the nuclear large rRNA transcript • transcription from RNA polymerase I promoter • transcription initiation from RNA polymerase I promoter • transcription elongation from RNA polymerase I promoter • termination of RNA polymerase I transcription • rRNA transcription • gene expression • regulation of gene expression, epigenetic • negative regulation of gene expression, epigenetic Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	64425	64424
Ensembl	ENSG00000137054	ENSMUSG00000028318
UniProt	Q9GZS1	Q8K202
RefSeq (mRNA)	NM_001282766	NM_001285800
RefSeq (protein)	NP_001269695	NP_001272729
Location (UCSC)	Chr 9: 37.49 – 37.5 Mb	Chr 4: 45.02 – 45.04 Mb
PubMed search

DNA-directed RNA polymerase I subunit RPA49 is an enzyme that in humans is encoded by the POLR1E gene.^[1][2]

Model organisms

Polr1e knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Abnormal
Recessive lethal study	Abnormal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology	Normal
Peripheral blood lymphocytes	Normal
Micronucleus test	Normal
Heart weight	Normal
Tail epidermis wholemount	Normal
Skin Histopathology	Normal
Brain histopathology	Normal
Eye Histopathology	Normal
Salmonella infection	Normal[3]
Citrobacter infection	Normal[4]
All tests and analysis from[5][6]

Model organisms have been used in the study of POLR1E function. A conditional knockout mouse line, called Polr1e^{tm1a(KOMP)Wtsi[7][8]} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.^[9][10][11]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.^[5][12] Twenty seven tests were carried out on mutant mice and two significant abnormalities were observed.^[5] No homozygous mutant embryos were identified during gestation, and therefore none survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice; no additional significant abnormalities were observed in these animals.^[5]

Interactions

POLR1E has been shown to interact with CD3EAP^[13] and POLR1C.^[14][13][15]

References

1. Hanada K, Song CZ, Yamamoto K, Yano K, Maeda Y, Yamaguchi K, Muramatsu M (May 1996). "RNA polymerase I associated factor 53 binds to the nucleolar transcription factor UBF and functions in specific rDNA transcription". The EMBO Journal 15 (9): 2217–26. PMC 450146. PMID 8641287.
2. "Entrez Gene: POLR1E polymerase (RNA) I polypeptide E, 53kDa".
3. "Salmonella infection data for Polr1e". Wellcome Trust Sanger Institute.
4. "Citrobacter infection data for Polr1e". Wellcome Trust Sanger Institute.
5. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
6. Mouse Resources Portal, Wellcome Trust Sanger Institute.
7. "International Knockout Mouse Consortium".
8. "Mouse Genome Informatics".
9. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
10. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
11. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
12. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biology 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353.
13. Yamamoto K, Yamamoto M, Hanada K, Nogi Y, Matsuyama T, Muramatsu M (Jul 2004). "Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription". Molecular and Cellular Biology 24 (14): 6338–49. doi:10.1128/MCB.24.14.6338-6349.2004. PMC 434256. PMID 15226435.
14. Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
15. Seither P, Zatsepina O, Hoffmann M, Grummt I (Sep 1997). "Constitutive and strong association of PAF53 with RNA polymerase I". Chromosoma 106 (4): 216–25. doi:10.1007/s004120050242. PMID 9254723.

Further reading

• Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Seither P, Zatsepina O, Hoffmann M, Grummt I (Sep 1997). "Constitutive and strong association of PAF53 with RNA polymerase I". Chromosoma 106 (4): 216–25. doi:10.1007/s004120050242. PMID 9254723.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Voit R, Grummt I (Nov 2001). "Phosphorylation of UBF at serine 388 is required for interaction with RNA polymerase I and activation of rDNA transcription". Proceedings of the National Academy of Sciences of the United States of America 98 (24): 13631–6. doi:10.1073/pnas.231071698. PMC 61092. PMID 11698641.
• Scherl A, Couté Y, Déon C, Callé A, Kindbeiter K, Sanchez JC, Greco A, Hochstrasser D, Diaz JJ (Nov 2002). "Functional proteomic analysis of human nucleolus". Molecular Biology of the Cell 13 (11): 4100–9. doi:10.1091/mbc.E02-05-0271. PMC 133617. PMID 12429849.
• Bjerregaard B, Wrenzycki C, Strejcek F, Laurincik J, Holm P, Ochs RL, Rosenkranz C, Callesen H, Rath D, Niemann H, Maddox-Hyttel P (Apr 2004). "Expression of nucleolar-related proteins in porcine preimplantation embryos produced in vivo and in vitro". Biology of Reproduction 70 (4): 867–76. doi:10.1095/biolreprod.103.021683. PMID 14585813.
• Baran V, Pavlok A, Bjerregaard B, Wrenzycki C, Hermann D, Philimonenko VV, Lapathitis G, Hozak P, Niemann H, Motlik J (Apr 2004). "Immunolocalization of upstream binding factor and pocket protein p130 during final stages of bovine oocyte growth". Biology of Reproduction 70 (4): 877–86. doi:10.1095/biolreprod.103.018408. PMID 14613906.
• Bjerregaard B, Wrenzycki C, Philimonenko VV, Hozak P, Laurincik J, Niemann H, Motlik J, Maddox-Hyttel P (Apr 2004). "Regulation of ribosomal RNA synthesis during the final phases of porcine oocyte growth". Biology of Reproduction 70 (4): 925–35. doi:10.1095/biolreprod.103.020941. PMID 14627545.
• Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G (Feb 2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nature Cell Biology 6 (2): 97–105. doi:10.1038/ncb1086. PMID 14743216.
• Yamamoto K, Yamamoto M, Hanada K, Nogi Y, Matsuyama T, Muramatsu M (Jul 2004). "Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription". Molecular and Cellular Biology 24 (14): 6338–49. doi:10.1128/MCB.24.14.6338-6349.2004. PMC 434256. PMID 15226435.
• Percipalle P, Fomproix N, Cavellán E, Voit R, Reimer G, Krüger T, Thyberg J, Scheer U, Grummt I, Farrants AK (May 2006). "The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription". EMBO Reports 7 (5): 525–30. doi:10.1038/sj.embor.7400657. PMC 1479564. PMID 16514417.
• Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.