CYTH2

Cytohesin 2
PDB rendering based on 1pbv.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1PBV​, 1R8M​, 1R8Q​, 1R8S​, 1S9D​, 4JMI​, 4JMO​, 4JWL​, 4JXH​, 4L5M​
Identifiers
Symbols	CYTH2 ; ARNO; CTS18; CTS18.1; PSCD2; PSCD2L; SEC7L; Sec7p-L; Sec7p-like
External IDs	OMIM: 602488 MGI: 1334255 HomoloGene: 32116 ChEMBL: 5995 GeneCards: CYTH2 Gene
Gene ontology Molecular function • ARF guanyl-nucleotide exchange factor activity • protein binding • lipid binding • inositol 1,4,5 trisphosphate binding Cellular component • cytoplasm • trans-Golgi network • plasma membrane • membrane • growth cone Biological process • endocytosis • actin cytoskeleton organization • regulation of cell adhesion • regulation of ARF protein signal transduction • positive regulation of GTPase activity Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	9266	19158
Ensembl	ENSG00000105443	ENSMUSG00000003269
UniProt	Q99418	P63034
RefSeq (mRNA)	NM_004228	NM_001112701
RefSeq (protein)	NP_004219	NP_001106171
Location (UCSC)	Chr 19: 48.47 – 48.48 Mb	Chr 7: 45.81 – 45.81 Mb
PubMed search

Cytohesin-2 is a protein that in humans is encoded by the CYTH2 gene.^[1][2][3]

Function

Cytohesin-2 (CYTH2), formerly known as Pleckstrin homology, Sec7 and coiled/coil domains 2 (PSCD2), is a member of the cytohesin family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of CYTHs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. CYTH2 exhibits GEP activity in vitro with ARF1, ARF3, and ARF6. CYTH2 protein is 83% homologous to CYTH1. Two transcript variants encoding different isoforms have been found for this gene.^[3]

Interactions

CYTH2 has been shown to interact with Arrestin beta 2^[4] and Arrestin beta 1.^[4]

References

1. Kolanus W, Nagel W, Schiller B, Zeitlmann L, Godar S, Stockinger H, Seed B (Sep 1996). "Alpha L beta 2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1, a cytoplasmic regulatory molecule". Cell 86 (2): 233–42. doi:10.1016/S0092-8674(00)80095-1. PMID 8706128.
2. Chardin P, Paris S, Antonny B, Robineau S, Béraud-Dufour S, Jackson CL, Chabre M (Jan 1997). "A human exchange factor for ARF contains Sec7- and pleckstrin-homology domains". Nature 384 (6608): 481–4. doi:10.1038/384481a0. PMID 8945478. Check date values in: |year= / |date= mismatch (help)
3. "Entrez Gene: PSCD2 pleckstrin homology, Sec7 and coiled-coil domains 2 (cytohesin-2)".
4. Claing A, Chen W, Miller WE, Vitale N, Moss J, Premont RT, Lefkowitz RJ (Nov 2001). "beta-Arrestin-mediated ADP-ribosylation factor 6 activation and beta 2-adrenergic receptor endocytosis". J. Biol. Chem. 276 (45): 42509–13. doi:10.1074/jbc.M108399200. PMID 11533043.

Further reading

• Meacci E, Tsai SC, Adamik R, Moss J, Vaughan M (1997). "Cytohesin-1, a cytosolic guanine nucleotide-exchange protein for ADP-ribosylation factor". Proc. Natl. Acad. Sci. U.S.A. 94 (5): 1745–8. doi:10.1073/pnas.94.5.1745. PMC 19987. PMID 9050849.
• Frank S, Upender S, Hansen SH, Casanova JE (1998). "ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6". J. Biol. Chem. 273 (1): 23–7. doi:10.1074/jbc.273.1.23. PMID 9417041.
• Mossessova E, Gulbis JM, Goldberg J (1998). "Structure of the guanine nucleotide exchange factor Sec7 domain of human arno and analysis of the interaction with ARF GTPase". Cell 92 (3): 415–23. doi:10.1016/S0092-8674(00)80933-2. PMID 9476900.
• Cherfils J, Ménétrey J, Mathieu M, Le Bras G, Robineau S, Béraud-Dufour S, Antonny B, Chardin P (1998). "Structure of the Sec7 domain of the Arf exchange factor ARNO". Nature 392 (6671): 101–5. doi:10.1038/32210. PMID 9510256.
• Venkateswarlu K, Oatey PB, Tavaré JM, Cullen PJ (1998). "Insulin-dependent translocation of ARNO to the plasma membrane of adipocytes requires phosphatidylinositol 3-kinase". Curr. Biol. 8 (8): 463–6. doi:10.1016/S0960-9822(98)70181-2. PMID 9550703.
• Kim HS (1998). "Assignment of the human cts18.1 gene PSCD2L to chromosome 19 band q13 using a radiation hybrid mapping panel". Cytogenet. Cell Genet. 83 (1-2): 43. doi:10.1159/000015163. PMID 9925920.
• Santy LC, Frank SR, Hatfield JC, Casanova JE (1999). "Regulation of ARNO nucleotide exchange by a PH domain electrostatic switch". Curr. Biol. 9 (20): 1173–6. doi:10.1016/S0960-9822(00)80019-6. PMID 10531036.
• Ogasawara M, Kim SC, Adamik R, Togawa A, Ferrans VJ, Takeda K, Kirby M, Moss J, Vaughan M (2000). "Similarities in function and gene structure of cytohesin-4 and cytohesin-1, guanine nucleotide-exchange proteins for ADP-ribosylation factors". J. Biol. Chem. 275 (5): 3221–30. doi:10.1074/jbc.275.5.3221. PMID 10652308.
• Smith JS, Tachibana I, Pohl U, Lee HK, Thanarajasingam U, Portier BP, Ueki K, Ramaswamy S, Billings SJ, Mohrenweiser HW, Louis DN, Jenkins RB (2000). "A transcript map of the chromosome 19q-arm glioma tumor suppressor region". Genomics 64 (1): 44–50. doi:10.1006/geno.1999.6101. PMID 10708517.
• Vitale N, Pacheco-Rodriguez G, Ferrans VJ, Riemenschneider W, Moss J, Vaughan M (2000). "Specific functional interaction of human cytohesin-1 and ADP-ribosylation factor domain protein (ARD1)". J. Biol. Chem. 275 (28): 21331–9. doi:10.1074/jbc.M909642199. PMID 10748148.
• Macia E, Paris S, Chabre M (2000). "Binding of the PH and polybasic C-terminal domains of ARNO to phosphoinositides and to acidic lipids". Biochemistry 39 (19): 5893–901. doi:10.1021/bi992795w. PMID 10801341.
• Lee SY, Pohajdak B (2000). "N-terminal targeting of guanine nucleotide exchange factors (GEF) for ADP ribosylation factors (ARF) to the Golgi". J. Cell. Sci. 113 ( Pt 11) (11): 1883–9. PMID 10806099.
• Claing A, Chen W, Miller WE, Vitale N, Moss J, Premont RT, Lefkowitz RJ (2001). "beta-Arrestin-mediated ADP-ribosylation factor 6 activation and beta 2-adrenergic receptor endocytosis". J. Biol. Chem. 276 (45): 42509–13. doi:10.1074/jbc.M108399200. PMID 11533043.
• Kitano J, Kimura K, Yamazaki Y, Soda T, Shigemoto R, Nakajima Y, Nakanishi S (2002). "Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins". J. Neurosci. 22 (4): 1280–9. PMID 11850456.
• Mansour M, Lee SY, Pohajdak B (2002). "The N-terminal coiled coil domain of the cytohesin/ARNO family of guanine nucleotide exchange factors interacts with the scaffolding protein CASP". J. Biol. Chem. 277 (35): 32302–9. doi:10.1074/jbc.M202898200. PMID 12052827.
• Buchet-Poyau K, Mehenni H, Radhakrishna U, Antonarakis SE (2002). "Search for the second Peutz-Jeghers syndrome locus: exclusion of the STK13, PRKCG, KLK10, and PSCD2 genes on chromosome 19 and the STK11IP gene on chromosome 2". Cytogenet. Genome Res. 97 (3-4): 171–8. doi:10.1159/000066620. PMID 12438709.
• Huh M, Han JH, Lim CS, Lee SH, Kim S, Kim E, Kaang BK (2003). "Regulation of neuritogenesis and synaptic transmission by msec7-1, a guanine nucleotide exchange factor, in cultured Aplysia neurons". J. Neurochem. 85 (1): 282–5. doi:10.1046/j.1471-4159.2003.01718.x. PMID 12641750.