PUF60

Poly-U binding splicing factor 60KDa
PDB rendering based on 2dny.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2DNY​, 2KXF​, 2KXH​, 2QFJ​, 3DXB​, 3UE2​, 3US5​, 3UWT​
Identifiers
Symbols	PUF60 ; FIR; RoBPI; SIAHBP1; VRJS
External IDs	OMIM: 604819 MGI: 1915209 HomoloGene: 8614 GeneCards: PUF60 Gene
Gene ontology Molecular function • nucleotide binding • DNA binding • protein binding • identical protein binding • poly(A) RNA binding Cellular component • nucleoplasm • mitochondrion • Golgi apparatus • cyclin-dependent protein kinase activating kinase holoenzyme complex • ribonucleoprotein complex Biological process • transcription, DNA-templated • regulation of transcription, DNA-templated • mRNA processing • apoptotic process • RNA splicing Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	22827	67959
Ensembl	ENSG00000179950	ENSMUSG00000002524
UniProt	Q9UHX1	Q3UEB3
RefSeq (mRNA)	NM_001136033	NM_001164600
RefSeq (protein)	NP_001129505	NP_001158072
Location (UCSC)	Chr 8: 143.82 – 143.83 Mb	Chr 15: 76.07 – 76.08 Mb
PubMed search

Poly(U)-binding-splicing factor PUF60 is a protein that in humans is encoded by the PUF60 gene.^[1][2][3][4]

The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.^[4]

Interactions

PUF60 has been shown to interact with U2AF2.^[5][6]

References

1. Bouffard P, Barbar E, Briere F, Boire G (Feb 2000). "Interaction cloning and characterization of RoBPI, a novel protein binding to human Ro ribonucleoproteins". RNA 6 (1): 66–78. doi:10.1017/S1355838200990277. PMC 1369894. PMID 10668799.
2. Liu J, He L, Collins I, Ge H, Libutti D, Li J, Egly JM, Levens D (Jul 2000). "The FBP interacting repressor targets TFIIH to inhibit activated transcription". Mol Cell 5 (2): 331–41. doi:10.1016/S1097-2765(00)80428-1. PMID 10882074.
3. Hastings ML, Allemand E, Duelli DM, Myers MP, Krainer AR (Jun 2007). Valcarcel, Juan, ed. "Control of pre-mRNA splicing by the general splicing factors PUF60 and U2AF65". PLoS ONE 2 (6): e538. doi:10.1371/journal.pone.0000538. PMC 1888729. PMID 17579712.
4. "Entrez Gene: SIAHBP1 fuse-binding protein-interacting repressor".
5. Rual, Jean-François; Venkatesan Kavitha, Hao Tong, Hirozane-Kishikawa Tomoko, Dricot Amélie, Li Ning, Berriz Gabriel F, Gibbons Francis D, Dreze Matija, Ayivi-Guedehoussou Nono, Klitgord Niels, Simon Christophe, Boxem Mike, Milstein Stuart, Rosenberg Jennifer, Goldberg Debra S, Zhang Lan V, Wong Sharyl L, Franklin Giovanni, Li Siming, Albala Joanna S, Lim Janghoo, Fraughton Carlene, Llamosas Estelle, Cevik Sebiha, Bex Camille, Lamesch Philippe, Sikorski Robert S, Vandenhaute Jean, Zoghbi Huda Y, Smolyar Alex, Bosak Stephanie, Sequerra Reynaldo, Doucette-Stamm Lynn, Cusick Michael E, Hill David E, Roth Frederick P, Vidal Marc (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature (England) 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. Cite uses deprecated parameter |coauthors= (help)
6. Ewing, Rob M; Chu Peter, Elisma Fred, Li Hongyan, Taylor Paul, Climie Shane, McBroom-Cerajewski Linda, Robinson Mark D, O'Connor Liam, Li Michael, Taylor Rod, Dharsee Moyez, Ho Yuen, Heilbut Adrian, Moore Lynda, Zhang Shudong, Ornatsky Olga, Bukhman Yury V, Ethier Martin, Sheng Yinglun, Vasilescu Julian, Abu-Farha Mohamed, Lambert Jean-Philippe, Duewel Henry S, Stewart Ian I, Kuehl Bonnie, Hogue Kelly, Colwill Karen, Gladwish Katharine, Muskat Brenda, Kinach Robert, Adams Sally-Lin, Moran Michael F, Morin Gregg B, Topaloglou Thodoros, Figeys Daniel (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. (England) 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. Cite uses deprecated parameter |coauthors= (help)

Further reading

• Page-McCaw PS, Amonlirdviman K, Sharp PA (2000). "PUF60: a novel U2AF65-related splicing activity". RNA 5 (12): 1548–60. doi:10.1017/S1355838299991938. PMC 1369877. PMID 10606266.
• Poleev A, Hartmann A, Stamm S (2000). "A trans-acting factor, isolated by the three-hybrid system, that influences alternative splicing of the amyloid precursor protein minigene". Eur. J. Biochem. 267 (13): 4002–10. doi:10.1046/j.1432-1327.2000.01431.x. PMID 10866799.
• Liu J, Akoulitchev S, Weber A, et al. (2001). "Defective interplay of activators and repressors with TFIH in xeroderma pigmentosum". Cell 104 (3): 353–63. doi:10.1016/S0092-8674(01)00223-9. PMID 11239393.
• Sandrock B, Egly JM (2001). "A yeast four-hybrid system identifies Cdk-activating kinase as a regulator of the XPD helicase, a subunit of transcription factor IIH". J. Biol. Chem. 276 (38): 35328–33. doi:10.1074/jbc.M105570200. PMID 11445587.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Jin J, Smith FD, Stark C, et al. (2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Curr. Biol. 14 (16): 1436–50. doi:10.1016/j.cub.2004.07.051. PMID 15324660.
• Stelzl U, Worm U, Lalowski M, et al. (2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. PMID 16169070.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514.
• Liu J, Kouzine F, Nie Z, et al. (2006). "The FUSE/FBP/FIR/TFIIH system is a molecular machine programming a pulse of c-myc expression". EMBO J. 25 (10): 2119–30. doi:10.1038/sj.emboj.7601101. PMC 1462968. PMID 16628215.
• Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569.
• Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.