para-Nitrophenylphosphate

para-Nitrophenylphosphate

Names

IUPAC name

(4-nitrophenyl) dihydrogen phosphate

Other names

pNPP

Identifiers

CAS Number

330-13-2^N

ChEBI

CHEBI:17440^N

ChEMBL

ChEMBL24231^N

Jmol interactive 3D

Image

PubChem

378

SMILES

C1=CC(=CC=C1[N+](=O)[O-])OP(=O)(O)O

Properties

Chemical formula

C₆H₆NO₆P

Molar mass

219.088701

Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

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Infobox references

para-Nitrophenylphosphate (pNPP) is a chromogenic substrate for acid and alkaline phosphatase in ELISA and conventional spectrophotometric assays. These enzymes catalyse the hydrolysis of pNPP to yellow para-nitrophenol. This product absorbs strongly at 405 nm and can be measured with a visible light spectrophotometer.

PNPP+H_2O \; \overrightarrow{_{AP}} \; PNP + P_i

External links

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