Peptide nucleic acid

Peptide nucleic acid (PNA) is an artificially synthesized polymer similar to DNA or RNA invented by Peter E. Nielsen (Univ. Copenhagen), Michael Egholm (Univ. Copenhagen), Rolf H. Berg (Risø National Lab), and Ole Buchardt (Univ. Copenhagen) in 1991.[1] The name is somewhat of a misnomer as PNA is not an acid.

Synthetic peptide nucleic acid oligomers have been used in recent years in molecular biology procedures, diagnostic assays, and antisense therapies. Due to their higher binding strength it is not necessary to design long PNA oligomers for use in these roles, which usually require oligonucleotide probes of 20–25 bases. The main concern of the length of the PNA-oligomers is to guarantee the specificity. PNA oligomers also show greater specificity in binding to complementary DNAs, with a PNA/DNA base mismatch being more destabilizing than a similar mismatch in a DNA/DNA duplex. This binding strength and specificity also applies to PNA/RNA duplexes. PNAs are not easily recognized by either nucleases or proteases, making them resistant to degradation by enzymes. PNAs are also stable over a wide pH range. Though an unmodified PNA cannot readily cross cell membranes to enter the cytosol, covalently coupling a cell penetrating peptide to a PNA can improve cytosolic delivery.

PNA is not known to occur naturally but N-(2-aminoethyl)-glycine (AEG), the backbone of PNA, are possibly an early form of genetic molecules for Life on Earth and produced by cyanobacteria.[2]

Structure

DNA and RNA have a deoxyribose and ribose sugar backbone, respectively, whereas PNA's backbone is composed of repeating N-(2-aminoethyl)-glycine units linked by peptide bonds. The various purine and pyrimidine bases are linked to the backbone by a methylene bridge (-CH
2-) and a carbonyl group (-(C=O)-). PNAs are depicted like peptides, with the N-terminus at the first (left) position and the C-terminus at the last (right) position.[3]

PNA binding

Since the backbone of PNA contains no charged phosphate groups, the binding between PNA/DNA strands is stronger than between DNA/DNA strands due to the lack of electrostatic repulsion. Unfortunately, this also causes it to be rather hydrophobic, which makes it difficult to deliver to body cells in solution without being flushed out of the body first. Early experiments with homopyrimidine strands (strands consisting of only one repeated pyrimidine base) have shown that the Tm ("melting" temperature) of a 6-base thymine PNA/adenine DNA double helix was 31 °C in comparison to an equivalent 6-base DNA/DNA duplex that denatures at a temperature less than 10 °C. Mixed base PNA molecules are true mimics of DNA molecules in terms of base-pair recognition. PNA/PNA binding is stronger than PNA/DNA binding.

Delivery

In 2015 Jain et. al. described a trans-acting DNA-based amphiphatic delivery system for convenient delivery of poly A tailed uncharged nucleic acids (UNA) such as PNAs and morpholinos, so that several UNA’s can be easily screened ex vivo.[4]

PNA world hypothesis

It has been hypothesized that the earliest life on Earth may have used PNA as a genetic material due to its extreme robustness, simpler formation, and possible spontaneous polymerization at 100°C[5] (while water at standard pressure boils at this temperature, water at high pressure—as in deep ocean—boils at higher temperatures). If this is so, life evolved to a DNA/RNA-based system only at a later stage.[6][7] Evidence for this PNA world hypothesis is however far from conclusive. See RNA world for related information.[8]

Applications

See also

References

  1. ↑ Nielsen PE, Egholm M, Berg RH, Buchardt O (December 1991). "Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide". Science 254 (5037): 1497–500. doi:10.1126/science.1962210. PMID 1962210.
  2. ↑ Cyanobacteria Produce N-(2-Aminoethyl)Glycine, a Backbone for Peptide Nucleic Acids Which May Have Been the First Genetic Molecules for Life on Earth
  3. ↑ Egholm M, Buchardt O, Christensen L, Behrens C, Freier SM, Driver DA, Berg RH, Kim SK, Nordén B, and Nielsen PE (1993). "PNA Hybridizes to Complementary Oligonucleotides Obeying the Watson-Crick Hydrogen Bonding Rules". Nature 365 (6446): 566–8. doi:10.1038/365566a0. PMID 7692304.
  4. ↑ Harsh V. Jain, D. Verthelyi, and S. L. Beaucage. 'Amphipathic Trans-Acting Phosphorothioate DNA Elements Mediate The Delivery Of Uncharged Nucleic Acid Sequences In Mammalian Cells'. RSC Adv. 5.80 (2015): 65245-65254. http://dx.doi.org/10.1039/C5RA12038A
  5. ↑ Wittung P, Nielsen PE, Buchardt Ole, Egholm M, and Nordén B (1994). "DNA-like Double Helix formed by Peptide Nucleic Acid". Nature 368 (6471): 561–3. doi:10.1038/368561a0. PMID 8139692.
  6. ↑ Nelson KE, Levy M, and Miller SL (2000). "Peptide nucleic acids rather than RNA may have been the first genetic molecule". Proc. Natl. Acad. Sci. USA 97 (8): 3868–71. doi:10.1073/pnas.97.8.3868. PMC 18108. PMID 10760258.
  7. ↑ Alberts, Johnson, Lewis, Raff, Roberts, and Walter (March 2002). Molecular Biology of the Cell (4th ed.). Routledge. ISBN 0-8153-3218-1.
  8. ↑ Zimmer C (January 2009). "On the Origin of Life on Earth". Science 323 (5911): 198–9. doi:10.1126/science.323.5911.198. PMID 19131603.
  9. ↑ Anstaett P, Gasser G (2014). "Peptide nucleic acid - an opportunity for bio-nanotechnology". Chimia 68 (4): 264–8. doi:10.2533/chimia.2014.264. PMID 24983612.

Further reading

External links

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