PITRM1

Pitrilysin metallopeptidase 1
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 4L3T​, 4NGE​
Identifiers
Symbols	PITRM1 ; MP1; PreP
External IDs	MGI: 1916867 HomoloGene: 5742 GeneCards: PITRM1 Gene
Gene ontology Molecular function • metalloendopeptidase activity • enzyme activator activity • zinc ion binding Cellular component • mitochondrion • mitochondrial matrix Biological process • proteolysis • protein processing • positive regulation of catalytic activity Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	10531	69617
Ensembl	ENSG00000107959	ENSMUSG00000021193
UniProt	Q5JRX3	Q8K411
RefSeq (mRNA)	NM_001242307	NM_145131
RefSeq (protein)	NP_001229236	NP_660113
Location (UCSC)	Chr 10: 3.14 – 3.17 Mb	Chr 13: 6.55 – 6.58 Mb
PubMed search

Pitrilysin metallopeptidase 1 also known as presequence protease, mitochondrial (PreP) and metalloprotease 1 (MTP-1) is an enzyme that in humans is encoded by the PITRM1 gene.^[1][2][3][4] It is also sometimes called metalloprotease 1 (MP1; ).

Model organisms

Model organisms have been used in the study of PITRM1 function. A conditional knockout mouse line called Pitrm1^{tm1a(KOMP)Wtsi} was generated at the Wellcome Trust Sanger Institute.^[5] Male and female animals underwent a standardized phenotypic screen^[6] to determine the effects of deletion.^{[7][8][9][10]} Additional screens performed: - In-depth immunological phenotyping^[11]

Pitrm1 knockout mouse phenotype

Characteristic	Phenotype
All data available at.[6][11]
Peripheral blood leukocytes 6 Weeks	Normal
Haematology 6 Weeks	Abnormal
Insulin	Normal
Homozygous viability at P14	Abnormal
Recessive lethal study	Abnormal
Body weight	Normal
Neurological assessment	Normal
Grip strength	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology 16 Weeks	Normal
Peripheral blood leukocytes 16 Weeks	Normal
Heart weight	Normal
Salmonella infection	Normal
Cytotoxic T Cell Function	Normal
Spleen Immunophenotyping	Normal
Mesenteric Lymph Node Immunophenotyping	Normal
Bone Marrow Immunophenotyping	Normal
Epidermal Immune Composition	Normal
Trichuris Challenge	Normal

References

1. Marusov EV (Jul 1977). "[Ecological sterotypes of defensive behavior in fish under the action of chemical danger signals]". Nauchnye Doklady Vyssheĭ Shkoly. Biologicheskie Nauki (8): 67–9. PMID 1036083.
2. Kikuno R, Nagase T, Ishikawa K, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Jun 1999). "Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research 6 (3): 197–205. doi:10.1093/dnares/6.3.197. PMID 10470851.
3. Falkevall A, Alikhani N, Bhushan S, Pavlov PF, Busch K, Johnson KA, Eneqvist T, Tjernberg L, Ankarcrona M, Glaser E (Sep 2006). "Degradation of the amyloid beta-protein by the novel mitochondrial peptidasome, PreP". The Journal of Biological Chemistry 281 (39): 29096–104. doi:10.1074/jbc.M602532200. PMID 16849325.
4. "Entrez Gene: PITRM1 pitrilysin metallopeptidase 1".
5. Gerdin AK (2010). "The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x.
6. "International Mouse Phenotyping Consortium".
7. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (Jun 2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–42. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.
8. Dolgin E (Jun 2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718.
9. Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247.
10. White JK, Gerdin AK, Karp NA, Ryder E, Buljan M, Bussell JN, Salisbury J, Clare S, Ingham NJ, Podrini C, Houghton R, Estabel J, Bottomley JR, Melvin DG, Sunter D, Adams NC, Tannahill D, Logan DW, Macarthur DG, Flint J, Mahajan VB, Tsang SH, Smyth I, Watt FM, Skarnes WC, Dougan G, Adams DJ, Ramirez-Solis R, Bradley A, Steel KP (Jul 2013). "Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes". Cell 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC 3717207. PMID 23870131.
11. "Infection and Immunity Immunophenotyping (3i) Consortium".

Further reading

• Schaeffer HJ, Catling AD, Eblen ST, Collier LS, Krauss A, Weber MJ (Sep 1998). "MP1: a MEK binding partner that enhances enzymatic activation of the MAP kinase cascade". Science 281 (5383): 1668–71. doi:10.1126/science.281.5383.1668. PMID 9733512.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (Oct 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Maruyama K, Sugano S (Jan 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.