Polony (biology)
Polony is a contraction of "polymerase colony," a small colony of DNA.
Polonies are discrete clonal amplifications of a single DNA molecule, grown in a gel matrix. This approach greatly improves the signal-to-noise ratio. Polonies can be generated using several techniques that include solid-phase PCR in polyacrylamide gels. However, other earlier patented technologies, such as that from Manteia Predictive Medicine (acquired by Solexa), which generate DNA on a solid phase surface by bridge amplification - are generally referred to as "Clusters".
The terminology and distinction between 'polony' and 'cluster' have become confused recently. Growth of clonal copies of DNA on bead surfaces remains to be generically named although some also seek to name this technique as a "polony" method. The concept of localizing and analyzing regions containing clonal nucleic acid populations was first described in patents by Brown, et al.. (assigned to Genomic Nanosystems), however these are in liquid phase. Clusters are distinct in that they are based on solid-phase amplification of single DNA molecules where the DNA has been covalently attached to a surface. This technology,initially coined "DNA colony generation", had been invented and developed in late 1996 at Glaxo-Welcome's Geneva Biomedical Research Institute (GBRI), by Dr Pascal Mayer and Dr Laurent Farinelli,[1] and was publicly presented for the first time in 1998.[2] It was finally brought to market by Solexa. Solexa Ltd/INC (Bentley et al.).
In one conceptually simple way to create polonies, a solution containing DNA fragments is poured onto a microscope slide dilute enough so that individual molecules are separated. DNA polymerase is added, which copies each fragment repeatedly, creating millions of polonies, each of which contains only copies of the original fragment of DNA. These "polonies" are then used for various kinds of DNA research like DNA sequencing, although this method is not used in any currently commercially available sequencer.
References
- ↑ patents WO 9844151, WO 9844152
- ↑ DNA colony massively parallel sequencing ams98 presentation
External links
- Polony technology guide
- "Methods of nucleic acid amplification and sequencing".
- Adessi C, Matton G, Ayala G, et al. (October 2000). "Solid phase DNA amplification: characterisation of primer attachment and amplification mechanisms". Nucleic Acids Res. 28 (20): E87. doi:10.1093/nar/28.20.e87. PMC 110803. PMID 11024189.