Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)

Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)
Identifiers
EC number	4.3.3.6
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing) (EC 4.3.3.6, PdxST) is an enzyme with systematic name D-ribose 5-phosphate,D-glyceraldehyde 3-phosphate pyridoxal 5'-phosphate-lyase.^{[1][2][3][4][5][6][7][8]} This enzyme catalyses the following chemical reaction

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine $\rightleftharpoons$ pyridoxal 5'-phosphate + L-glutamate + 3 H₂O + phosphate (overall reaction)

(1a) L-glutamine + H₂O $\rightleftharpoons$ L-glutamate + NH₃

(1b) D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + NH3 $\rightleftharpoons$ pyridoxal 5'-phosphate + 4 H₂O + phosphate

The enzyme can also use ribulose 5-phosphate and dihydroxyacetone phosphate.

References

1. Burns, K.E., Xiang, Y., Kinsland, C.L., McLafferty, F.W. and Begley, T.P. (2005). "Reconstitution and biochemical characterization of a new pyridoxal-5′-phosphate biosynthetic pathway". J. Am. Chem. Soc. 127: 3682–3683. doi:10.1021/ja042792t. PMID 15771487.
2. Raschle, T., Amrhein, N. and Fitzpatrick, T.B. (2005). "On the two components of pyridoxal 5′-phosphate synthase from Bacillus subtilis". J. Biol. Chem. 280: 32291–32300. doi:10.1074/jbc.m501356200. PMID 16030023.
3. Strohmeier, M., Raschle, T., Mazurkiewicz, J., Rippe, K., Sinning, I., Fitzpatrick, T.B. and Tews, I. (2006). "Structure of a bacterial pyridoxal 5′-phosphate synthase complex". Proc. Natl. Acad. Sci. USA 103: 19284–19289. doi:10.1073/pnas.0604950103. PMID 17159152.
4. Raschle, T., Arigoni, D., Brunisholz, R., Rechsteiner, H., Amrhein, N. and Fitzpatrick, T.B. (2007). "Reaction mechanism of pyridoxal 5′-phosphate synthase. Detection of an enzyme-bound chromophoric intermediate". J. Biol. Chem. 282: 6098–6105. doi:10.1074/jbc.m610614200. PMID 17189272.
5. Hanes, J.W., Keresztes, I. and Begley, T.P. (2008). "Trapping of a chromophoric intermediate in the Pdx1-catalyzed biosynthesis of pyridoxal 5′-phosphate". Angew. Chem. Int. Ed. Engl. 47: 2102–2105. doi:10.1002/anie.200704390. PMID 18260082.
6. Hanes, J.W., Burns, K.E., Hilmey, D.G., Chatterjee, A., Dorrestein, P.C. and Begley, T.P. (2008). "Mechanistic studies on pyridoxal phosphate synthase: the reaction pathway leading to a chromophoric intermediate". J. Am. Chem. Soc. 130: 3043–3052. doi:10.1021/ja076604l. PMID 18271580.
7. Hanes, J.W., Keresztes, I. and Begley, T.P. (2008). "¹³C NMR snapshots of the complex reaction coordinate of pyridoxal phosphate synthase". Nat. Chem. Biol. 4: 425–430. doi:10.1038/nchembio.93. PMID 18516049.
8. Wallner, S., Neuwirth, M., Flicker, K., Tews, I. and Macheroux, P. (2009). "Dissection of contributions from invariant amino acids to complex formation and catalysis in the heteromeric pyridoxal 5-phosphate synthase complex from Bacillus subtilis". Biochemistry 48: 1928–1935. doi:10.1021/bi801887r. PMID 19152323.

External links

• Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing) at the US National Library of Medicine Medical Subject Headings (MeSH)