RAB5A

RAB5A, member RAS oncogene family
PDB rendering based on 1huq.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 1N6H​, 1N6I​, 1N6K​, 1N6L​, 1N6N​, 1N6O​, 1N6P​, 1N6R​, 1R2Q​, 1TU3​, 1TU4​, 3MJH​, 4Q9U​
Identifiers
Symbols	RAB5A ; RAB5
External IDs	OMIM: 179512 MGI: 105926 HomoloGene: 68142 GeneCards: RAB5A Gene
Gene ontology Molecular function • GTPase activity • protein binding • GTP binding • GDP binding Cellular component • ruffle • cytoplasm • endosome • early endosome • cytosol • plasma membrane • synaptic vesicle • endosome membrane • actin cytoskeleton • endocytic vesicle • axon • dendrite • somatodendritic compartment • melanosome • neuronal cell body • terminal bouton • axon terminus • membrane raft • phagocytic vesicle • extracellular exosome • cytoplasmic side of early endosome membrane Biological process • intracellular protein transport • endocytosis • blood coagulation • regulation of endocytosis • Rab protein signal transduction • synaptic vesicle recycling • early endosome to late endosome transport • positive regulation of exocytosis • regulation of endosome size • regulation of filopodium assembly • receptor internalization involved in canonical Wnt signaling pathway • regulation of synaptic vesicle exocytosis Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	5868	271457
Ensembl	ENSG00000144566	ENSMUSG00000017831
UniProt	P20339	Q9CQD1
RefSeq (mRNA)	NM_001292048	NM_025887
RefSeq (protein)	NP_001278977	NP_080163
Location (UCSC)	Chr 3: 19.95 – 19.99 Mb	Chr 17: 53.48 – 53.51 Mb
PubMed search

Ras-related protein Rab-5A is a protein that in humans is encoded by the RAB5A gene.^[1][2]

Function

RAB5A localizes to early endosomes where it is involved in the recruitment of RAB7A and the maturation of these compartments to late endosomes.^[3] It drives the maturation of endosomes by transporting vacuolar (H+)-ATPases (V-ATPases) from trans-Golgi network to endocytic vesicles.^[4]

Interactions

RAB5A has been shown to interact with:

• CHML,^[5][6]
• RABEP1,^{[7][8][9][10]}
• SDCBP,^[11] and
• ZFYVE20^[12]

References

1. Rousseau-Merck MF, Zahraoui A, Touchot N, Tavitian A, Berger R (Feb 1991). "Chromosome assignment of four RAS-related RAB genes". Human Genetics 86 (4): 350–4. doi:10.1007/BF00201831. PMID 1999336.
2. "Entrez Gene: RAB5A RAB5A, member RAS oncogene family".
3. Huotari J, Helenius A (Aug 2011). "Endosome maturation". The EMBO Journal 30 (17): 3481–500. doi:10.1038/emboj.2011.286. PMC 3181477. PMID 21878991.
4. Zhang C, Li A, Zhang X, Xiao H (Mar 2011). "A novel TIP30 protein complex regulates EGF receptor signaling and endocytic degradation". The Journal of Biological Chemistry 286 (11): 9373–81. doi:10.1074/jbc.M110.207720. PMID 21252234.
5. Anant JS, Desnoyers L, Machius M, Demeler B, Hansen JC, Westover KD, Deisenhofer J, Seabra MC (Sep 1998). "Mechanism of Rab geranylgeranylation: formation of the catalytic ternary complex". Biochemistry 37 (36): 12559–68. doi:10.1021/bi980881a. PMID 9730828.
6. Cremers FP, Armstrong SA, Seabra MC, Brown MS, Goldstein JL (Jan 1994). "REP-2, a Rab escort protein encoded by the choroideremia-like gene". The Journal of Biological Chemistry 269 (3): 2111–7. PMID 8294464.
7. Xiao GH, Shoarinejad F, Jin F, Golemis EA, Yeung RS (Mar 1997). "The tuberous sclerosis 2 gene product, tuberin, functions as a Rab5 GTPase activating protein (GAP) in modulating endocytosis". The Journal of Biological Chemistry 272 (10): 6097–100. doi:10.1074/jbc.272.10.6097. PMID 9045618.
8. Vitale G, Rybin V, Christoforidis S, Thornqvist P, McCaffrey M, Stenmark H, Zerial M (Apr 1998). "Distinct Rab-binding domains mediate the interaction of Rabaptin-5 with GTP-bound Rab4 and Rab5". The EMBO Journal 17 (7): 1941–51. doi:10.1093/emboj/17.7.1941. PMC 1170540. PMID 9524117.
9. Stenmark H, Vitale G, Ullrich O, Zerial M (Nov 1995). "Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion". Cell 83 (3): 423–32. doi:10.1016/0092-8674(95)90120-5. PMID 8521472.
10. Valsdottir R, Hashimoto H, Ashman K, Koda T, Storrie B, Nilsson T (Nov 2001). "Identification of rabaptin-5, rabex-5, and GM130 as putative effectors of rab33b, a regulator of retrograde traffic between the Golgi apparatus and ER". FEBS Letters 508 (2): 201–9. doi:10.1016/s0014-5793(01)02993-3. PMID 11718716.
11. Tomoda T, Kim JH, Zhan C, Hatten ME (Mar 2004). "Role of Unc51.1 and its binding partners in CNS axon outgrowth". Genes & Development 18 (5): 541–58. doi:10.1101/gad.1151204. PMC 374236. PMID 15014045.
12. Nielsen E, Christoforidis S, Uttenweiler-Joseph S, Miaczynska M, Dewitte F, Wilm M, Hoflack B, Zerial M (Oct 2000). "Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain". The Journal of Cell Biology 151 (3): 601–12. doi:10.1083/jcb.151.3.601. PMC 2185588. PMID 11062261.

Further reading

• Bucci C, Parton RG, Mather IH, Stunnenberg H, Simons K, Hoflack B, Zerial M (Sep 1992). "The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway". Cell 70 (5): 715–28. doi:10.1016/0092-8674(92)90306-W. PMID 1516130.
• Zahraoui A, Touchot N, Chardin P, Tavitian A (Jul 1989). "The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion". The Journal of Biological Chemistry 264 (21): 12394–401. PMID 2501306.
• Farnsworth CC, Seabra MC, Ericsson LH, Gelb MH, Glomset JA (Dec 1994). "Rab geranylgeranyl transferase catalyzes the geranylgeranylation of adjacent cysteines in the small GTPases Rab1A, Rab3A, and Rab5A". Proceedings of the National Academy of Sciences of the United States of America 91 (25): 11963–7. doi:10.1073/pnas.91.25.11963. PMC 45356. PMID 7991565.
• Cremers FP, Armstrong SA, Seabra MC, Brown MS, Goldstein JL (Jan 1994). "REP-2, a Rab escort protein encoded by the choroideremia-like gene". The Journal of Biological Chemistry 269 (3): 2111–7. PMID 8294464.
• Stenmark H, Vitale G, Ullrich O, Zerial M (Nov 1995). "Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion". Cell 83 (3): 423–32. doi:10.1016/0092-8674(95)90120-5. PMID 8521472.
• Vita F, Soranzo MR, Borelli V, Bertoncin P, Zabucchi G (Sep 1996). "Subcellular localization of the small GTPase Rab5a in resting and stimulated human neutrophils". Experimental Cell Research 227 (2): 367–73. doi:10.1006/excr.1996.0286. PMID 8831575.
• Xiao GH, Shoarinejad F, Jin F, Golemis EA, Yeung RS (Mar 1997). "The tuberous sclerosis 2 gene product, tuberin, functions as a Rab5 GTPase activating protein (GAP) in modulating endocytosis". The Journal of Biological Chemistry 272 (10): 6097–100. doi:10.1074/jbc.272.10.6097. PMID 9045618.
• Simonsen A, Lippé R, Christoforidis S, Gaullier JM, Brech A, Callaghan J, Toh BH, Murphy C, Zerial M, Stenmark H (Jul 1998). "EEA1 links PI(3)K function to Rab5 regulation of endosome fusion". Nature 394 (6692): 494–8. doi:10.1038/28879. PMID 9697774.
• Anant JS, Desnoyers L, Machius M, Demeler B, Hansen JC, Westover KD, Deisenhofer J, Seabra MC (Sep 1998). "Mechanism of Rab geranylgeranylation: formation of the catalytic ternary complex". Biochemistry 37 (36): 12559–68. doi:10.1021/bi980881a. PMID 9730828.
• Bucci C, Chiariello M, Lattero D, Maiorano M, Bruni CB (May 1999). "Interaction cloning and characterization of the cDNA encoding the human prenylated rab acceptor (PRA1)". Biochemical and Biophysical Research Communications 258 (3): 657–62. doi:10.1006/bbrc.1999.0651. PMID 10329441.
• Callaghan J, Nixon S, Bucci C, Toh BH, Stenmark H (Oct 1999). "Direct interaction of EEA1 with Rab5b". European Journal of Biochemistry / FEBS 265 (1): 361–6. doi:10.1046/j.1432-1327.1999.00743.x. PMID 10491193.
• Shisheva A, Chinni SR, DeMarco C (Sep 1999). "General role of GDP dissociation inhibitor 2 in membrane release of Rab proteins: modulations of its functional interactions by in vitro and in vivo structural modifications". Biochemistry 38 (36): 11711–21. doi:10.1021/bi990200r. PMID 10512627.
• Masuda ES, Luo Y, Young C, Shen M, Rossi AB, Huang BC, Yu S, Bennett MK, Payan DG, Scheller RH (Mar 2000). "Rab37 is a novel mast cell specific GTPase localized to secretory granules". FEBS Letters 470 (1): 61–4. doi:10.1016/S0014-5793(00)01288-6. PMID 10722846.
• Hoffenberg S, Liu X, Nikolova L, Hall HS, Dai W, Baughn RE, Dickey BF, Barbieri MA, Aballay A, Stahl PD, Knoll BJ (Aug 2000). "A novel membrane-anchored Rab5 interacting protein required for homotypic endosome fusion". The Journal of Biological Chemistry 275 (32): 24661–9. doi:10.1074/jbc.M909600199. PMID 10818110.
• Nielsen E, Christoforidis S, Uttenweiler-Joseph S, Miaczynska M, Dewitte F, Wilm M, Hoflack B, Zerial M (Oct 2000). "Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and recruited to endosomes through a FYVE finger domain". The Journal of Cell Biology 151 (3): 601–12. doi:10.1083/jcb.151.3.601. PMC 2185588. PMID 11062261.
• Hartley JL, Temple GF, Brasch MA (Nov 2000). "DNA cloning using in vitro site-specific recombination". Genome Research 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
• Lanzetti L, Rybin V, Malabarba MG, Christoforidis S, Scita G, Zerial M, Di Fiore PP (Nov 2000). "The Eps8 protein coordinates EGF receptor signalling through Rac and trafficking through Rab5". Nature 408 (6810): 374–7. doi:10.1038/35042605. PMID 11099046.
• Wolf RM, Wilkes JJ, Chao MV, Resh MD (Oct 2001). "Tyrosine phosphorylation of p190 RhoGAP by Fyn regulates oligodendrocyte differentiation". Journal of Neurobiology 49 (1): 62–78. doi:10.1002/neu.1066. PMID 11536198.
• Seachrist JL, Laporte SA, Dale LB, Babwah AV, Caron MG, Anborgh PH, Ferguson SS (Jan 2002). "Rab5 association with the angiotensin II type 1A receptor promotes Rab5 GTP binding and vesicular fusion". The Journal of Biological Chemistry 277 (1): 679–85. doi:10.1074/jbc.M109022200. PMID 11682489.