RASGRP3

RAS guanyl releasing protein 3 (calcium and DAG-regulated)
Identifiers
Symbols	RASGRP3 ; GRP3
External IDs	OMIM: 609531 MGI: 3028579 HomoloGene: 15019 ChEMBL: 3638 GeneCards: RASGRP3 Gene
Gene ontology Molecular function • signal transducer activity • guanyl-nucleotide exchange factor activity • Ras guanyl-nucleotide exchange factor activity • GTPase activator activity • calcium ion binding • protein binding • Ras GTPase binding • kinase binding • diacylglycerol binding Cellular component • plasma membrane • integral component of plasma membrane • guanyl-nucleotide exchange factor complex • perinuclear region of cytoplasm Biological process • MAPK cascade • small GTPase mediated signal transduction • Ras protein signal transduction • positive regulation of GTPase activity Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	25780	240168
Ensembl	ENSG00000152689	ENSMUSG00000071042
UniProt	Q8IV61	Q6NZH9
RefSeq (mRNA)	NM_001139488	NM_001166493
RefSeq (protein)	NP_001132960	NP_001159965
Location (UCSC)	Chr 2: 33.44 – 33.56 Mb	Chr 17: 75.44 – 75.53 Mb
PubMed search

Ras guanyl-releasing protein 3 is a protein that in humans is encoded by the RASGRP3 gene.^[1][2][3]

Function

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RASGRP3, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000).^[3]

Interactions

RASGRP3 has been shown to interact with PRKCD.^[4]

References

1. Nagase T, Ishikawa K, Suyama M, Kikuno R, Hirosawa M, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O (Dec 1998). "Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research 5 (6): 355–64. doi:10.1093/dnares/5.6.355. PMID 10048485.
2. Rebhun JF, Castro AF, Quilliam LA (Nov 2000). "Identification of guanine nucleotide exchange factors (GEFs) for the Rap1 GTPase. Regulation of MR-GEF by M-Ras-GTP interaction". The Journal of Biological Chemistry 275 (45): 34901–8. doi:10.1074/jbc.M005327200. PMID 10934204.
3. "Entrez Gene: RASGRP3 RAS guanyl releasing protein 3 (calcium and DAG-regulated)".
4. Brodie C, Steinhart R, Kazimirsky G, Rubinfeld H, Hyman T, Ayres JN, Hur GM, Toth A, Yang D, Garfield SH, Stone JC, Blumberg PM (Jul 2004). "PKCdelta associates with and is involved in the phosphorylation of RasGRP3 in response to phorbol esters". Molecular Pharmacology 66 (1): 76–84. doi:10.1124/mol.66.1.76. PMID 15213298.

Further reading

• Hartley JL, Temple GF, Brasch MA (Nov 2000). "DNA cloning using in vitro site-specific recombination". Genome Research 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
• Lorenzo PS, Kung JW, Bottorff DA, Garfield SH, Stone JC, Blumberg PM (Feb 2001). "Phorbol esters modulate the Ras exchange factor RasGRP3". Cancer Research 61 (3): 943–9. PMID 11221888.
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A (Mar 2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Research 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
• Teixeira C, Stang SL, Zheng Y, Beswick NS, Stone JC (Aug 2003). "Integration of DAG signaling systems mediated by PKC-dependent phosphorylation of RasGRP3". Blood 102 (4): 1414–20. doi:10.1182/blood-2002-11-3621. PMID 12730099.
• Brodie C, Steinhart R, Kazimirsky G, Rubinfeld H, Hyman T, Ayres JN, Hur GM, Toth A, Yang D, Garfield SH, Stone JC, Blumberg PM (Jul 2004). "PKCdelta associates with and is involved in the phosphorylation of RasGRP3 in response to phorbol esters". Molecular Pharmacology 66 (1): 76–84. doi:10.1124/mol.66.1.76. PMID 15213298.
• Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A (Oct 2004). "From ORFeome to biology: a functional genomics pipeline". Genome Research 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
• Aiba Y, Oh-hora M, Kiyonaka S, Kimura Y, Hijikata A, Mori Y, Kurosaki T (Nov 2004). "Activation of RasGRP3 by phosphorylation of Thr-133 is required for B cell receptor-mediated Ras activation". Proceedings of the National Academy of Sciences of the United States of America 101 (47): 16612–7. doi:10.1073/pnas.0407468101. PMC 528733. PMID 15545601.
• Roberts DM, Anderson AL, Hidaka M, Swetenburg RL, Patterson C, Stanford WL, Bautch VL (Dec 2004). "A vascular gene trap screen defines RasGRP3 as an angiogenesis-regulated gene required for the endothelial response to phorbol esters". Molecular and Cellular Biology 24 (24): 10515–28. doi:10.1128/MCB.24.24.10515-10528.2004. PMC 533983. PMID 15572660.
• Zheng Y, Liu H, Coughlin J, Zheng J, Li L, Stone JC (May 2005). "Phosphorylation of RasGRP3 on threonine 133 provides a mechanistic link between PKC and Ras signaling systems in B cells". Blood 105 (9): 3648–54. doi:10.1182/blood-2004-10-3916. PMID 15657177.
• Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S (Jan 2006). "The LIFEdb database in 2006". Nucleic Acids Research 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.
• Okamura SM, Oki-Idouchi CE, Lorenzo PS (Nov 2006). "The exchange factor and diacylglycerol receptor RasGRP3 interacts with dynein light chain 1 through its C-terminal domain". The Journal of Biological Chemistry 281 (47): 36132–9. doi:10.1074/jbc.M605093200. PMID 17012239.
• Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Molecular Systems Biology 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.
• Randhawa PK, Rylova S, Heinz JY, Kiser S, Fried JH, Dunworth WP, Anderson AL, Barber AT, Chappell JC, Roberts DM, Bautch VL (May 2011). "The Ras activator RasGRP3 mediates diabetes-induced embryonic defects and affects endothelial cell migration". Circulation Research 108 (10): 1199–208. doi:10.1161/CIRCRESAHA.110.230888. PMID 21474816.