RPS27A

Ribosomal protein S27a

PDB rendering based on 1aar.

Available structures

Ortholog search: PDBe, RCSB

List of PDB id codes
2KHW, 2KOX, 2KTF, 2KWU, 2KWV, 2L0F, 2L0T, 2XK5, 3AXC, 3I3T, 3K9P, 3N30, 3N32, 3NHE, 3NOB, 3NS8, 3PHD, 3PHW, 3TBL, 3VDZ, 4V6X, 5AJ0

Identifiers

Symbols

RPS27A ; CEP80; HEL112; S27A; UBA80; UBC; UBCEP1; UBCEP80

External IDs

OMIM: 191343 MGI: 1925544 HomoloGene: 37715 GeneCards: RPS27A Gene

Gene ontology
Molecular function	• structural constituent of ribosome • protein binding • poly(A) RNA binding • metal ion binding
Cellular component	• extracellular space • nucleus • nucleoplasm • nucleolus • cytoplasm • cytosol • plasma membrane • endosome membrane • small ribosomal subunit • membrane • cytosolic small ribosomal subunit • endocytic vesicle membrane • extracellular exosome
Biological process	• G1/S transition of mitotic cell cycle • G2/M transition of mitotic cell cycle • negative regulation of transcription from RNA polymerase II promoter • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay • activation of MAPK activity • protein polyubiquitination • mitotic cell cycle • stimulatory C-type lectin receptor signaling pathway • toll-like receptor signaling pathway • antigen processing and presentation of peptide antigen via MHC class I • antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent • MyD88-dependent toll-like receptor signaling pathway • MyD88-independent toll-like receptor signaling pathway • carbohydrate metabolic process • glycogen biosynthetic process • glucose metabolic process • DNA repair • transcription, DNA-templated • transcription initiation from RNA polymerase II promoter • translation • translational initiation • translational elongation • translational termination • SRP-dependent cotranslational protein targeting to membrane • apoptotic process • DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest • epidermal growth factor receptor signaling pathway • transforming growth factor beta receptor signaling pathway • Notch signaling pathway • Notch receptor processing • I-kappaB kinase/NF-kappaB signaling • JNK cascade • circadian rhythm • fibroblast growth factor receptor signaling pathway • gene expression • regulation of necrotic cell death • programmed cell death • viral process • endosomal transport • viral life cycle • virion assembly • viral protein processing • viral transcription • cytokine-mediated signaling pathway • translesion synthesis • negative regulation of transforming growth factor beta receptor signaling pathway • anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process • regulation of type I interferon production • negative regulation of type I interferon production • positive regulation of type I interferon production • toll-like receptor 2 signaling pathway • toll-like receptor 3 signaling pathway • toll-like receptor 4 signaling pathway • toll-like receptor 5 signaling pathway • toll-like receptor 9 signaling pathway • toll-like receptor 10 signaling pathway • ion transmembrane transport • TRIF-dependent toll-like receptor signaling pathway • nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway • Fc-epsilon receptor signaling pathway • toll-like receptor TLR1:TLR2 signaling pathway • toll-like receptor TLR6:TLR2 signaling pathway • negative regulation of epidermal growth factor receptor signaling pathway • error-prone translesion synthesis • antigen processing and presentation of exogenous peptide antigen via MHC class I • DNA damage response, detection of DNA damage • regulation of apoptotic process • positive regulation of apoptotic process • negative regulation of apoptotic process • positive regulation of I-kappaB kinase/NF-kappaB signaling • cellular protein metabolic process • small molecule metabolic process • innate immune response • positive regulation of transcription from RNA polymerase II promoter • neurotrophin TRK receptor signaling pathway • T cell receptor signaling pathway • positive regulation of NF-kappaB transcription factor activity • stress-activated MAPK cascade • negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle • positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition • regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle • transmembrane transport • membrane organization • regulation of transcription from RNA polymerase II promoter in response to hypoxia • necroptotic process • nucleotide-binding oligomerization domain containing signaling pathway • error-free translesion synthesis • cellular response to hypoxia • intracellular transport of virus • negative regulation of canonical Wnt signaling pathway • positive regulation of canonical Wnt signaling pathway • apoptotic signaling pathway • programmed necrotic cell death
Sources: Amigo / QuickGO

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 2:
55.23 – 55.24 Mb

Chr 11:
29.55 – 29.55 Mb

PubMed search

40S ribosomal protein S27a is a protein that in humans is encoded by the RPS27A gene.^[1]^[2]

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi.^[2]

References

↑ Kenmochi N, Kawaguchi T, Rozen S, Davis E, Goodman N, Hudson TJ, Tanaka T, Page DC (Aug 1998). "A map of 75 human ribosomal protein genes". Genome Res 8 (5): 509–23. doi:10.1101/gr.8.5.509. PMID 9582194.
1 2 "Entrez Gene: RPS27A ribosomal protein S27a".

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Kanayama H, Tanaka K, Aki M, et al. (1992). "Changes in expressions of proteasome and ubiquitin genes in human renal cancer cells". Cancer Res. 51 (24): 6677–85. PMID 1660345.
Monia BP, Ecker DJ, Jonnalagadda S, et al. (1989). "Gene synthesis, expression, and processing of human ubiquitin carboxyl extension proteins". J. Biol. Chem. 264 (7): 4093–103. PMID 2537304.
Redman KL, Rechsteiner M (1989). "Identification of the long ubiquitin extension as ribosomal protein S27a". Nature 338 (6214): 438–40. doi:10.1038/338438a0. PMID 2538756.
Lund PK, Moats-Staats BM, Simmons JG, et al. (1985). "Nucleotide sequence analysis of a cDNA encoding human ubiquitin reveals that ubiquitin is synthesized as a precursor". J. Biol. Chem. 260 (12): 7609–13. PMID 2581967.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Vladimirov SN, Ivanov AV, Karpova GG, et al. (1996). "Characterization of the human small-ribosomal-subunit proteins by N-terminal and internal sequencing, and mass spectrometry". Eur. J. Biochem. 239 (1): 144–9. doi:10.1111/j.1432-1033.1996.0144u.x. PMID 8706699.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Kirschner LS, Stratakis CA (2000). "Structure of the human ubiquitin fusion gene Uba80 (RPS27a) and one of its pseudogenes". Biochem. Biophys. Res. Commun. 270 (3): 1106–10. doi:10.1006/bbrc.2000.2568. PMID 10772958.
Petersen BO, Wagener C, Marinoni F, et al. (2000). "Cell cycle– and cell growth–regulated proteolysis of mammalian CDC6 is dependent on APC–CDH1". Genes Dev. 14 (18): 2330–43. doi:10.1101/gad.832500. PMC 316932. PMID 10995389.
Bolton D, Evans PA, Stott K, Broadhurst RW (2002). "Structure and properties of a dimeric N-terminal fragment of human ubiquitin". J. Mol. Biol. 314 (4): 773–87. doi:10.1006/jmbi.2001.5181. PMID 11733996.
Yoshihama M, Uechi T, Asakawa S, et al. (2002). "The Human Ribosomal Protein Genes: Sequencing and Comparative Analysis of 73 Genes". Genome Res. 12 (3): 379–90. doi:10.1101/gr.214202. PMC 155282. PMID 11875025.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Cohen BD, Bariteau JT, Magenis LM, Dias JA (2003). "Regulation of follitropin receptor cell surface residency by the ubiquitin-proteasome pathway". Endocrinology 144 (10): 4393–402. doi:10.1210/en.2002-0063. PMID 12960054.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Li H, Seth A (2004). "An RNF11: Smurf2 complex mediates ubiquitination of the AMSH protein". Oncogene 23 (10): 1801–8. doi:10.1038/sj.onc.1207319. PMID 14755250.

PDB gallery

1aar: STRUCTURE OF A DIUBIQUITIN CONJUGATE AND A MODEL FOR INTERACTION WITH UBIQUITIN CONJUGATING ENZYME (E2)

1cmx: STRUCTURAL BASIS FOR THE SPECIFICITY OF UBIQUITIN C-TERMINAL HYDROLASES

1d3z: UBIQUITIN NMR STRUCTURE

1f9j: STRUCTURE OF A NEW CRYSTAL FORM OF TETRAUBIQUITIN

1fxt: STRUCTURE OF A CONJUGATING ENZYME-UBIQUITIN THIOLESTER COMPLEX

1g6j: STRUCTURE OF RECOMBINANT HUMAN UBIQUITIN IN AOT REVERSE MICELLES

1gjz: SOLUTION STRUCTURE OF A DIMERIC N-TERMINAL FRAGMENT OF HUMAN UBIQUITIN

1nbf: Crystal structure of a UBP-family deubiquitinating enzyme in isolation and in complex with ubiquitin aldehyde

1ogw: SYNTHETIC UBIQUITIN WITH FLUORO-LEU AT 50 AND 67

1otr: Solution Structure of a CUE-Ubiquitin Complex

1p3q: Mechanism of Ubiquitin Recognition by the CUE Domain of VPS9

1q0w: Solution structure of Vps27 amino-terminal UIM-ubiquitin complex

1q5w: Ubiquitin Recognition by Npl4 Zinc-Fingers

1s1q: TSG101(UEV) domain in complex with Ubiquitin

1sif: Crystal structure of a multiple hydrophobic core mutant of ubiquitin

1tbe: STRUCTURE OF TETRAUBIQUITIN SHOWS HOW MULTIUBIQUITIN CHAINS CAN BE FORMED

1ubi: SYNTHETIC STRUCTURAL AND BIOLOGICAL STUDIES OF THE UBIQUITIN SYSTEM. PART 1

1ubq: STRUCTURE OF UBIQUITIN REFINED AT 1.8 ANGSTROMS RESOLUTION

1ud7: SOLUTION STRUCTURE OF THE DESIGNED HYDROPHOBIC CORE MUTANT OF UBIQUITIN, 1D7

1uzx: A COMPLEX OF THE VPS23 UEV WITH UBIQUITIN

1v80: Solution structures of ubiquitin at 30 bar and 3 kbar

1v81: Solution structures of ubiquitin at 30 bar and 3 kbar

1wr1: The complex structure of Dsk2p UBA with ubiquitin

1wr6: Crystal structure of GGA3 GAT domain in complex with ubiquitin

1wrd: Crystal structure of Tom1 GAT domain in complex with ubiquitin

1xd3: Crystal structure of UCHL3-UbVME complex

1xqq: Simultaneous determination of protein structure and dynamics

1yd8: COMPLEX OF HUMAN GGA3 GAT DOMAIN AND UBIQUITIN

1yiw: X-ray Crystal Structure of a Chemically Synthesized Ubiquitin

1yj1: X-ray Crystal Structure of a Chemically Synthesized [D-Gln35]Ubiquitin

1yx5: Solution Structure of S5a UIM-1/Ubiquitin Complex

1yx6: Solution Structure of S5a UIM-2/Ubiquitin Complex

1zgu: Solution structure of the human Mms2-Ubiquitin complex

2ayo: Structure of USP14 bound to ubquitin aldehyde

2bgf: NMR STRUCTURE OF LYS48-LINKED DI-UBIQUITIN USING CHEMICAL SHIFT PERTURBATION DATA TOGETHER WITH RDCS AND 15N-RELAXATION DATA

2c7m: HUMAN RABEX-5 RESIDUES 1-74 IN COMPLEX WITH UBIQUITIN

2c7n: HUMAN RABEX-5 RESIDUES 1-74 IN COMPLEX WITH UBIQUITIN

2d3g: Double sided ubiquitin binding of Hrs-UIM

2den: Solution Structure of the Ubiquitin-Associated Domain of Human BMSC-UbP and its Complex with Ubiquitin

2dx5: The complex structure between the mouse EAP45-GLUE domain and ubiquitin

2fcm: X-ray Crystal Structure of a Chemically Synthesized [D-Gln35]Ubiquitin with a Cubic Space Group

2fcn: X-ray Crystal Structure of a Chemically Synthesized [D-Val35]Ubiquitin with a Cubic Space Group

2fcq: X-ray Crystal Structure of a Chemically Synthesized Ubiquitin with a Cubic Space Group

2fcs: X-ray Crystal Structure of a Chemically Synthesized [L-Gln35]Ubiquitin with a Cubic Space Group

2fid: Crystal Structure of a Bovine Rabex-5 fragment complexed with ubiquitin

2fif: Crystal Structure of a Bovine Rabex-5 fragment complexed with ubiquitin

2fuh: Solution Structure of the UbcH5c/Ub Non-covalent Complex

2g3q: Solution Structure of Ede1 UBA-ubiquitin complex

2g45: Co-crystal structure of znf ubp domain from the deubiquitinating enzyme isopeptidase T (isot) in complex with ubiquitin

2gbj: Crystal Structure of the 9-10 8 Glycine Insertion Mutant of Ubiquitin.

2gbk: Crystal Structure of the 9-10 MoaD Insertion Mutant of Ubiquitin

2gbm: Crystal Structure of the 35-36 8 Glycine Insertion Mutant of Ubiquitin

2gbn: Crystal Structure of the 35-36 8 Glycine Insertion Mutant of Ubiquitin

2gbr: Crystal Structure of the 35-36 MoaD Insertion Mutant of Ubiquitin

2gmi: Mms2/Ubc13~Ubiquitin

2hd5: USP2 in complex with ubiquitin

2hth: Structural basis for ubiquitin recognition by the human EAP45/ESCRT-II GLUE domain

2ibi: Covalent Ubiquitin-USP2 Complex

2j7q: CRYSTAL STRUCTURE OF THE UBIQUITIN-SPECIFIC PROTEASE ENCODED BY MURINE CYTOMEGALOVIRUS TEGUMENT PROTEIN M48 IN COMPLEX WITH A UBQUITIN-BASED SUICIDE SUBSTRATE

2nr2: The MUMO (minimal under-restraining minimal over-restraining) method for the determination of native states ensembles of proteins

2o6v: Crystal structure and solution NMR studies of Lys48-linked tetraubiquitin at neutral pH

2oob: crystal structure of the UBA domain from Cbl-b ubiquitin ligase in complex with ubiquitin

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RPS27A

References

Further reading