Random hexamer

A random hexamer or random hexonucleotides are for various PCR applications such as rolling circle amplification to prime the DNA.

They are oligonucleotide sequences of 6 bases which are synthesised entirely randomly to give a numerous range of sequences that have the potential to anneal at many random points on a DNA sequence and act as a primer to commence first strand cDNA synthesis. [1][2][3]

References

  1. ↑ http://www.invitrogen.com/site/us/en/home/References/protocols/nucleic-acid-amplification-and-expression-profiling/pcr-protocol/pcr-and-rt-pcr.html
  2. ↑ https://www.thermoscientificbio.com/general-reagents-and-accessories/primers-for-cdna-synthesis
  3. ↑ Biases due to random hexamer priming: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2896536/
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