Rhizobium bangladeshense
| Rhizobium bangladeshense | |
|---|---|
| Scientific classification | |
| Kingdom: | Bacteria |
| Phylum: | Proteobacteria |
| Class: | Alphaproteobacteria |
| Order: | Rhizobiales |
| Family: | Rhizobiaceae |
| Genus: | Rhizobium |
| Species: | R. bangladeshense |
| Binomial name | |
| Rhizobium bangladeshense Harun-or Rashid et al. 2015 | |
Description of Rhizobium bangladeshense
Rhizobium bangladeshense, (ban.gla.desh.en'se. N.L. neut. adj. bangladeshense, from Bangladesh, referring to the geographical origin of the strains) is soil inhabitant, Gram negative, aerobic, non-spore forming, rod shaped and plant root nodule forming bacteria. This species has been described by Harun-or Rashid et al (2015) from lentil root nodules in Bangladesh. Different housekeeping genes (recA, atpD, glnII) have 91 - 96% similarity with close relatives (Rhizobium etli and Rhizobum phaseoli)although the 16S rRNA gene has about 99% similarities.
Type strain has 49 - 62% genetic relatedness with their close relatives in DNA-DNA hybridization experiment. Average nucleotide identity of whole genome sequences has 88- 89% similarities with close relatives. Colonies are circular, convex and creamy white on YEMA medium. Strains can tolerate pH values between 5.5 and 10 and are sensitive to ampicillin, resistant to kanamycin and nalidixic acid, and grow well in YEMA medium containing 0.5 % NaCl. Strains do not tolerate tetracycline and do not show any growth on LB medium. Type strain is BLR175T (= LMG 28442T = DSMZ 29287T). The DNA G+C content of type strain is 61 %.
Fatty acid composition of type strain is 16:0, 16:0 3-OH, 18:0, 19:0 cyclo ω8c, summed featured 2 and summed featured 8. Type strain could utilize D-maltose, D-trehalose, D-cellobiose, gentiobiose, sucrose, D-raffinose, α-D-glucose, D-turanose, α-D lactose, D-fructose, β-methyl-D-glucoside, salicin, N-acetyl-D-galactosamine, D-sorbitol, D-mannitol, D-arbitol, glycerol, D-glucose-6-phosphate, D-gluconic acid, quinic acid, D-saccharic acid, D-lactic acid methyl ester, lactic acid, α-keto-glutaric acid and tween 40. Type strain failed to utilize dextrin, D-aspertic acid, glycyl-L-proline, L-alanine, L-arginine, L-glutamic acid, L-histidine, L-serine, mucic acid, p-hydroxy-phenylacetic acid, methyl pyruvate, citric acid, D-malic acid, L-malic acid, propionic acid or formic acid. Type strain could grow in the presence of lincomycin and potassium tellurite, but not with 1% sodium lactate, troleandomycin, tetrazolium violet, tetrazolium blue, Nalidixic acid, lithium chloride and sodium butyrate.
Different gene sequences are available in NCBI and whole genome sequence in European nucleotide archive. Type strain was isolated from effective nodules of Lens culinaris from Khulna district of Bangladesh. Different strains of this species can form effective nodules and enhance growth of lentil, pea and lathyrus, and are useful for bio-fertilizer production.
References
1. Rashid, M.H., Young J.P.W., Everall, I., Clercx, P., Willems, A., Braun, M.S and Wink, W. (2015) Average nucleotide identity of genome sequences supports the description of Rhizobium lentis sp. nov., Rhizobium bangladeshense sp. nov. and Rhizobium binae sp. nov. from lentil (Lens culinaris) nodules. International Journal of Systematic and Evolutionary Microbiology, 65:3037-3045.
2. Rashid, M.H., Gonzalez, H., Young, J.P.W., and Wink, M. (2014) Rhizobium leguminosarum is the symbiont of lentil in the Middle East and Europe but not in Bangladesh. FEMS Microbiology Ecology, 87: 64 -77.
3. Rashid, M.H., Schafer, H., Gonzalez, H, and Wink, M. (2012) Genetic diversity of rhizobia nodulating lentil (Lens culinaris) in Bangladesh. Systematic and Applied Microbiology, 35: 98-109.